Acknowledgements - UCSF School of Dentistry - University of ...

Conclusion: While both proactive and passive phone education formats were acceptable to parents, the proactive format reached
a higher proportion of the sample population, particularly Spanish-speakers. This pilot study suggests proactive phone education
with oral health information might be of interest to low-income Spanish speaking parents of young children.
Support: USDHHS/NIH/NIDCR U54DE019285
#31 Title: Tobacco-Use Screening and Treatment in the UCSF School of Dentistry: A Pilot Study
K JARVI (1), D PIVNIK (2), T CHAI (3), K MEISELS (3), P CURL (4), L KROON L (5), J PROCHASKA (4), M WALSH (6)
(1) UCSF MS Graduate Program in Dental Hygiene Graduate Division Dental Hygiene (2) UCSF School of Dentistry, (3) UCSF School of
Pharmacy, (4) School of Medicine, (5) Vice Chair School of Pharmacy, (6) UCSF Department of Preventive and Restorative Dental
Sciences
Introduction: The leading cause of preventable death in the US, tobacco accounts for nearly 1 in 5 deaths or 443,000 annually (1). US
Clinical Practice Guidelines recommend clinicians assess tobacco use with all patients at all visits and provide evidence-based cessation
treatments.
Objective: To determine clinician tobacco-related screening and cessation practices at the UCSF Medical Center Outpatient clinics
Methods: A three-pronged cross-sectional assessment was conducted with a convenience sample of (1) clinic directors or their designee,
(2) clinicians, and (3) patients at UCSF outpatient medical and dental clinics.
Results: Assessments were completed with 11 of 15 dental clinic directors, 100 dental providers, and 100 of 110 approached dental
patients and with 12 out of 27 medical administrators, 36 medical providers, and 120 of 122 approached medical patients. Overall, 47% of
patients reported they were asked about tobacco use. Most clinicians reported they routinely ask about tobacco use (92%), while fewer
reported routinely advising users to quit (82%), assessing readiness to quit (57%), and assisting patients for tobacco cessation treatment
(54%). Less than half of the providers reported referral of patients to the California quitline. Few clinicians (2%) were aware of the UCSF
Tobacco Education Center (TEC) as a referral resource for cessation treatment. Top recommendations to increase clinical attention to
tobacco use were (1) prompts in the electronic medical record, (2) provision of revenue value unit incentives, and (3) greater publicizing of
referral resources like TEC.
Conclusion: UCSF medical and dental providers are performing above the national average for asking, advising, and assessing patients’
readiness to quit smoking, but practice shortcomings were found regarding providing cessation assistance and follow-up. Specific targets
for intervention are identified.
#32 Title: Epithelium-driven G protein signaling regulates the mineralization of mandibles
M H LE (1), J A HORST (2), E HSIAO (3), O KLEIN (2), P K DEN BESTEN (2), O V HORST (1)
(1) UCSF Department of Preventive and Restorative Dental Sciences, (2) UCSF Department of Orofacial Sciences, (3) UCSF
Department of Medicine
Background: G protein-coupled receptors (GPCRs) regulate many cellular activities related to development and differentiation.
However little is known about the influences of GPCRs on epithelial-mesenchymal interactions required for normal tooth and jaw
development. Many GPCRs signal through Gs proteins, which activate the production of second messenger cAMP.
Objective: Determine the effects of increasing Gs signals in epithelia on the formation of mesenchymal and epithelial tissues in
murine mandibles.
Methods: We used an engineered Gs-coupled GPCR known as receptor activated solely by synthetic ligands (RASSL) Rs1 to
investigate Gs protein activity in vivo with minimal interference from endogenous ligands. Double transgenic mice expressing Rs1 in
epithelial cells were created by mating K5-tTA mice with TetO-Rs1 mice. Mandibles were harvested six weeks after birth, scanned
using Micro X-ray Computed Tomography (MicroXCT), and examined histologically. MicroXCT scans were analyzed for relative
differences in mineral content using semi-automated algorithms to quantify volumetric pixel intensities of bone, dentin, and enamel.
Results: MicroXCT analyses of 6-week-old transgenic mice showed a significant decrease (p