Running UPLC

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10 Preparing to Run <strong>UPLC</strong>; Injection and Detection Paramters Pressure Assist Injection Sequence Step 3 – Position the sample Summary of Partial Loop using Pressure Assist Shorter Cycle Time than PLUNO No sample cushion Sample volume is conserved Recommended for large sample loop injections Performance dependency on weak wash solvent matching to mobile phase Air Gaps injected onto Column Accuracy is generally lower compared to Needle Overfill Has lower injection range, within a given loop for partial loop injections. Injection range is from 10 – 50% of the loop volume Accuracy and Precision are lower than Full Loop Mode Dr. Shula Levin, Medtechnica Pressure Assist Injection Sequence Step 4 – Injection valve injects sample Sample Loop contains Sample, air gaps and weak wash Partial Loop Using Needle Overfill
11 Summary of Partial Loop using Pressure Assist BENEFITS: — Short Cycle Time Preparing to Run <strong>UPLC</strong>; Injection and Detection Paramters — Sample volume is conserved — Recommended for large sample loop injections TRADEOFF: — Accuracy and Precision are lower than Full Loop Mode — Performance dependency on weak wash solvent matching to mobile phase — Accuracy is generally lower compared to Needle Overfill Partial Loop using Needle Overfill Step 1– Aspirate Sample and Air Gap Dr. Shula Levin, Medtechnica Partial Loop Using Needle Overfill Partial Loop using Needle Overfill Step 2 – Injection Sequence
Page 1 and 2: 1 םירטליפה םג ןכו , HP
Page 3 and 4: 3 Bottle Caps Preparing to Run UPLC
Page 5 and 6: 5 Preparing to Run UPLC; Injection
Page 7 and 8: 7 THREE injection methods available
Page 9: 9 Partial Loop Injection Weak Wash
Page 15 and 16: 15 Strong Contaminations Preparing
Page 21 and 22: 21 Teflon ® AF light guiding flow

Running UPLC

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