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2<br />
Preparing to Run <strong>UPLC</strong>; Injection and Detection Paramters<br />
General Operating Recommendations<br />
Use only Pyrex (Borosilicate 3.3) bottles. DURAN bottles<br />
dissolve at high pH (>9).<br />
Use highest quality solvents, water, buffers and additives.<br />
Flush buffers out of system with water after use (use 10-20%<br />
organic in water for storage).<br />
Keep all 4 solvent lines primed (use 10-20% organic in water for<br />
unused lines).<br />
Keep seal wash primed.<br />
Re-prime solvent lines before starting.<br />
Use 100 µL mixer for TFA/ACN gradients at low wavelengths.<br />
Solvent Filters<br />
Critical Clean<br />
Stainless Steel (7 pack)700003616<br />
Stainless Steel (1/pk) 700003615<br />
Titanium (7 pack) 700003530<br />
Titanium (1 pack) 700003546<br />
Solvent filter assembly 289002172<br />
Solvent filter insert 5 µm 700002756<br />
PAT (PEEK Alloyed with Teflon) filter<br />
PSL901292 2 µm<br />
PSL901294 5 µm<br />
Dr. Shula Levin, Medtechnica<br />
CORNING PYREX ® Type 1, Class A, Borosilicate Bottles<br />
SCHOTT DURAN ® Borosilicate Glass 3.3 Bottles<br />
Mobile Phase Preparation<br />
Filtration on membrane:<br />
Also: if Biolab solvents: Use ULC/MS grade only!<br />
Actions : Solvent degassing and filtration<br />
— Potential of impurities in filtration<br />
o GHP, PTFE, Nylon, or PVDF<br />
— Pores diameter : 0.2 µm.<br />
— Volatile additives may evaporate.<br />
NOTE: Refer to ‘Controlling Contamination in<br />
UltraPerformance LC/MS and HPLC Systems’<br />
715001307D available from Waters Corp.
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