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8<br />
Preparing to Run <strong>UPLC</strong>; Injection and Detection Paramters<br />
Full Loop Injection<br />
Step 3 – Position sample and overfill the loop<br />
Sample Loop contains only sample<br />
No Air Gaps<br />
No weak wash<br />
Summary of Full Loop injection mode<br />
Best accuracy and precision performance<br />
Needs multiple loop volumes of additional sample to be used per<br />
injection<br />
Larger overfill factors are required for smaller loops<br />
Need to change injection loops to vary volume<br />
Dr. Shula Levin, Medtechnica<br />
Full Loop Injection<br />
Step 4 – Sample Injection<br />
Summary of Full Loop<br />
Injection Mode<br />
BENEFITS<br />
— Best accuracy and precision performance<br />
TRADEOFF<br />
— Needs multiple loop volumes of additional sample to be used per<br />
injection<br />
o larger overfill factors are required for smaller loops (buffer zone<br />
improvement)<br />
— Need to change injection loops to vary volume
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