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5.5 Importance of dissolved organic carbon for phytoplankton nutrition in a eutrophic reservoir This study was supported by the Grant Agency of the Czech Republic (Project No. 206/07/P407) In 2008–2009, P. Znachor (znachy@hbu.cas.cz), Jiří Nedoma, and Pavel Rychtecký performed in situ experiments examining the effect of glucose addition on silica deposition kinetics and growth rates of Fragilaria crotonensis in the eutrophic Římov Reservoir (Czech Republic). Silica deposition kinetics was measured at four-hour intervals over a 24-hour incubation period with PDMPO (2-(4-pyridyl)-5{[4-dimethylaminoethyl-aminocarbamyl)- methoxy] phenyl}oxazole] fluorescence probe. A significant stimulatory effect of glucose supplemented at the concentration of 10 –4 mol l –1 on Fragilaria silification was observed at time 20 and 24 hours. Fragilaria growth rates almost doubled upon glucose enrichment compared to the untreated control at 24 h (Fig. 9). In addition, we conducted a dose-response experiment testing glucose additions from 10 –8 –10 –3 mol l –1 in 24-hour incubation. Glucose stimulated both Fragilaria silification and growth at concentrations >10 –7 mol l –1 (Fig. 10), which might occasionally occur in a reservoir as a result of e.g. accidental contamination of water by organic pollution [1]. Fig. 9: Kinetics of Fragilaria silification rates measured as PDMPO fluorescence at 4-hour intervals over 24 hours (a, c). Columns represent mean values (n ~ 50); bars are SEMs (standard errors of the mean). Fragilaria growth rates based on an increase in cell counts over 24 hours are given in the right panels (b, d). Asterisks denote significant differences between control and glucose amended samples (p
Fig. 10: Effect of various glucose additions on Fragilaria silification (a) and growth rates (b) measured after 24 hour incubation. Columns represent mean values; bars are standard errors of the mean. Asterisks denote significant differences between glucose-amended samples and untreated control (*p
Page 1 and 2: ACADEMY OF SCIENCES OF THE CZECH RE
Page 3 and 4: CONTENTS THE INSTITUTE, SCIENTIFIC
Page 5 and 6: Academy of Sciences of the Czech Re
Page 7 and 8: RNDr. Dagmar Sirová (maternity lea
Page 9 and 10: 1 INTRODUCTION 1.1 Directors’ pre
Page 11 and 12: 2008-2012 Reg. code 206/08/0015, Ec
Page 13 and 14: EQUIPPMET Income Reserve for invest
Page 15 and 16: 2 JAROSLAV HRBÁČEK (1921-2010) He
Page 17 and 18: 3 RESERVOIRS 3.1 Regular monitoring
Page 19 and 20: 3.3 Regular monitoring: fish stock
Page 21 and 22: Fig. 1: A - Schematic drawing of wa
Page 23 and 24: in spring and summer periods, mainl
Page 25 and 26: 3.7 Deep spawning of perch Perca fl
Page 27 and 28: In late summer, a sudden flood even
Page 29 and 30: 4 LAKES 4.1 Microbial loop componen
Page 31 and 32: 5 SPECIAL INVESTIGATIONS 5.1 Effect
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Page 35: pools in forest and alpine meadow s
Page 39 and 40: 1942 Jezberová, J., Jezbera, J., B
Page 41 and 42: B: International Proceedings or Mon
Page 43 and 44: 1980 Potužák, J.*, Duras, J.*, Bo
Page 45 and 46: APPENDIX Jaroslav Hrbáček (1921-2
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Page 51 and 52: Hrbáček, J., 1974: Vliv hnojení

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