Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...

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Preface x Power SYBR DRAFT ® Green PCR Master Mix Protocol November 1, 2006 2:07 pm, 7x9_Preface_Protocol.fm
Introduction 1 1 Overview This chapter describes the Power SYBR ® Green PCR Master Mix and provides important information on PCR practices. This chapter covers: Purpose of the Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2 Materials and Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4 Preventing Contamination and Nonspecific Amplification . . . . . .1-8 Amplicon Independent Amplification (Including Primer-Dimer) 1-11 Power SYBR ® Green PCR Master Mix Protocol 1-1 DRAFT September 29, 2005 1:41 pm, 1_Chapter.fm
Page 1 and 2: Power SYBR ® Green PCR Master Mix
Page 3 and 4: Contents Preface Safety . . . . . .
Page 5 and 6: Preface This preface contains: Safe
Page 7 and 8: Safety • Print Target - To print
Page 9: How to Obtain Support How to Obtain
Page 13 and 14: Purpose of the Kit Advantages of th
Page 15 and 16: Materials and Equipment Performance
Page 17 and 18: Materials and Equipment User-Suppli
Page 19 and 20: Preventing Contamination and Nonspe
Page 21 and 22: Amplicon Independent Amplification
Page 23 and 24: Amplicon Independent Amplification
Page 25 and 26: PCR 2 2 Overview This chapter descr
Page 27 and 28: . Designing Custom Target Sequences
Page 29 and 30: Amplifying Custom Target Sequences
Page 31 and 32: Reverse Transcription 3 3 Overview
Page 33 and 34: Reverse Transcription for All Ampli
Page 35 and 36: Reverse Transcription for the 18S A
Page 41 and 42: Optimizing Primer Concentrations 4
Page 43 and 44: Optimizing Primer Concentrations fo
Page 57 and 58: Data Analysis 5 5 Overview The chap
Page 59 and 60: Absolute and Relative Quantitation
Page 61 and 62:
Interpreting the Results a b Thresh
Page 63 and 64:
Interpreting the Results Threshold
Page 65 and 66:
Bibliography Delort, A.-M., Duplaa,
Page 68:
Worldwide Sales and Support Applied
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