Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
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Chapter 4<br />
Optimizing Primer Concentrations<br />
<strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong><br />
for Primer<br />
Optimization<br />
CHEMICAL HAZARD. <strong>Power</strong> <strong>SYBR</strong> <strong>Green</strong><br />
<strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong> is a combustible liquid <strong>and</strong> vapor (keep away from<br />
heat <strong>and</strong> flame). It may cause eye, skin, <strong>and</strong> respiratory tract<br />
irritation. Please read the MSDS, <strong>and</strong> follow the h<strong>and</strong>ling<br />
instructions. Wear appropriate protective eyewear, clothing <strong>and</strong><br />
gloves.<br />
<strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong> for Primer Optimization<br />
Component<br />
Volume (µL)<br />
for One 50-µL<br />
Reaction<br />
Volume (µL)<br />
for 100 50-µL<br />
Reactions<br />
Final<br />
Concentration<br />
<strong>Power</strong> <strong>SYBR</strong> <strong>Green</strong><br />
<strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong><br />
(2X)<br />
25 2500 1X<br />
Forward Primer Variable Variable 50 to 900 nM<br />
Reverse Primer Variable Variable 50 to 900 nM<br />
Template Variable Variable 1 to 100 ng<br />
Water Variable Variable —<br />
Total 50 5000 —<br />
4-4 <strong>Power</strong> <strong>SYBR</strong><br />
DRAFT<br />
® <strong>Green</strong> <strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong> <strong>Protocol</strong><br />
September 29, 2005 1:24 pm, 4_Chapter.fm
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