Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
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Chapter 1<br />
Introduction<br />
Materials<br />
Required but Not<br />
Supplied<br />
The items listed in the following tables are required in addition to the<br />
reagents supplied in the <strong>Power</strong> <strong>SYBR</strong> <strong>Green</strong> <strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong>.<br />
User-Supplied Materials<br />
Item<br />
AmpErase ® uracil-N-glycosylase<br />
(UNG)<br />
• Applied Biosystems 7900HT<br />
Real-Time <strong>PCR</strong> System<br />
• Applied Biosystems 7500<br />
Real-Time <strong>PCR</strong> System<br />
• Applied Biosystems 7300<br />
Real-Time <strong>PCR</strong> System<br />
• ABI PRISM ® 7700 Sequence<br />
Detection System (SDS)<br />
• ABI PRISM ® 7000 SDS<br />
• GeneAmp ® 5700 SDS<br />
Applied Biosystems 7900HT<br />
Real-Time <strong>PCR</strong> System 96-well<br />
Spectral Calibration Kit<br />
ABI PRISM ® 7000 Sequence<br />
Detection Systems Spectral<br />
Calibration Kit<br />
Sequence Detection Systems<br />
Spectral Calibration Kit<br />
(for 7700 instrument only)<br />
Sequence Detection Systems<br />
384-Well Spectral Calibration Kit<br />
Primer Express ® Software<br />
ABI PRISM ® 7700 Sequence<br />
Detection System User Bulletin #2:<br />
Relative Quantitation of Gene<br />
Expression<br />
ABI PRISM ® 384-Well Clear Optical<br />
Reaction Plate with Barcode<br />
Source<br />
Applied Biosystems<br />
(<strong>PN</strong> N808-0096)<br />
Contact your Applied Biosystems<br />
representative for the instrument<br />
best suited to meet your needs<br />
Applied Biosystems<br />
(<strong>PN</strong> 4328639)<br />
Applied Biosystems<br />
(<strong>PN</strong> 4328895)<br />
Applied Biosystems<br />
(<strong>PN</strong> 4305822)<br />
Applied Biosystems<br />
(<strong>PN</strong> 4323977)<br />
Contact your Applied Biosystems<br />
representative<br />
Applied Biosystems<br />
(<strong>PN</strong> 4303859)<br />
Applied Biosystems<br />
(<strong>PN</strong> 4309849)<br />
1-6 <strong>Power</strong> <strong>SYBR</strong><br />
DRAFT<br />
® <strong>Green</strong> <strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong> <strong>Protocol</strong><br />
September 29, 2005 1:41 pm, 1_Chapter.fm
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