Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
Power SYBR Green PCR Master Mix and RT-PCR Protocol (PN ...
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Chapter 3<br />
Reverse Transcription<br />
To conduct <strong>RT</strong> thermal cycling: (continued)<br />
Step<br />
Action<br />
3. Begin <strong>RT</strong>.<br />
IMPO<strong>RT</strong>ANT! After thermal cycling, store all cDNA samples at<br />
–15 to –25 °C.<br />
3-10 <strong>Power</strong> <strong>SYBR</strong><br />
DRAFT<br />
® <strong>Green</strong> <strong>PCR</strong> <strong>Master</strong> <strong>Mix</strong> <strong>Protocol</strong><br />
September 29, 2005 1:24 pm, 3_Chapter.fm
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