WHEAT FLOUR FORTIFICATION WITH IRON - Idpas.org

Table of Contents
Acknowledgments................................................................................................................................v
Foreword............................................................................................................................................vii
Abbreviations......................................................................................................................................ix
Glossary ..............................................................................................................................................xi
I. Introduction ....................................................................................................................................1
II. Qualitative Method.........................................................................................................................3
A. Spot test method.......................................................................................................................3
III. Semi-Quantitative Methods...........................................................................................................5
A. Spot test method.......................................................................................................................5
B. Colorimetric method ................................................................................................................5
IV. Quantitative Methods....................................................................................................................7
A. Spectrophotometric method .....................................................................................................7
B. Atomic absorption spectrophotometric (AAS) method ...........................................................7
Appendix 3.1: Cost of selected analytical instruments/equipment from different suppliers ...............9
Appendix 3.2: List of suppliers for laboratory glassware, equipment, and chemicals ......................11
Appendix 3.3: Iron qualitative method ..............................................................................................13
Appendix 3.4: Semi-quantitative spot test for iron, INCAP method IV............................................15
Appendix 3.5: Semi-quantitative colorimetric determination of iron in flour,
INCAP method V...........................................................................................................................17
Appendix 3.6: Iron spectrophotometric method, AACC method 40-41B .........................................21
Appendix 3.7: Spectrophotometric analysis for quantitative determination of iron in foods,
INCAP method VI .........................................................................................................................25
Appendix 3.8: Elements by atomic absorption spectrophotometry,
AACC method 40-70 .....................................................................................................................29
Appendix 3.9: AOAC official method 975.03, Metals in plants and pet foods,
Atomic absorption spectrophotometric method.................................................................................33
Appendix 3.10: Wet digestion using sulfuric and nitric acids ...........................................................37
Appendix 3.11: Laboratory safety .....................................................................................................39
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