Prof. Dr. Bernd Bukau Center for Molecular Biology ... - HBIGS

Prof. Dr. Bernd Bukau
Center for Molecular Biology Heidelberg (ZMBH)
University of Heidelberg
69120 Heidelberg
Phone: +49-(0) 6221-54 6795
Fax.: +49-(0) 6221-54 5894
E-mail: bukau@zmbh.uni-heidelberg.de
12/05/1954, Leipzig
CURRICULUM VITAE
2002 – present C4-Professor for Molecular Biology at the University of Heidelberg, ZMBH
1997 – 2002 C4-Professor for Biochemistry at the University of Freiburg, Institute
of Biochemistry and Molecular Biology, Faculty of Medicine
1994 Habilitation and appointment as "Universitätsdozent" for Microbiology and
Molecular Biology at the University of Heidelberg
1989 – 1997 Research assistant and project leader, ZMBH, University of Heidelberg
1986 – 1989 Postdoctoral fellow Massachusetts Institute of Technology, Cambridge
(USA)
1983 – 1986 Doctoral work, Dr. rer. nat. (1986), University of Konstanz
1977 Research stage, University of California, Santa Cruz (USA)
1974 – 1980 Biology School and Diploma Thesis (1980), University of Konstanz
COORDINATING FUNCTIONS
2006 – present Member of the EMBO Long-term Fellowship Committee
2005 – 2006 Coordinator of the Promotionskolleg Bioquant “Molecular machines:
mechanisms and functional interconnections“
2005 – present Director of the ZMBH
2004 – present Coordinating board of the SFB 638
2004 – present Member Rektoratskommission “BIOQUANT”
2003 – present Member of the Selection committee for the „Heinz Maier-Leibnitz-Prize“ of
the DFG
2003 – present Examination board of the Faculty of Biology (vice chair)
2002 – 2004 Deputy Director of the ZMBH
HONORS
2005 Leopoldina Forschungspreis
2005 Elected member of the Deutsche Akademie der Naturforscher Leopoldina
2001 Elected EMBO member
2000 Heinz Maier-Leibnitz-Prize of the DFG
FIELDS OF INTEREST
Protein folding and quality control; Molecular chaperones and proteases; Cellular stress
response.

CURRENTLY FUNDED PROJECTS
DFG-project on Mechanisms of the interaction between cytosolic proteases of E. coli and
substrates; DFG-project on Mechanism and protein disaggregation by the AAA+ chaperone
ClpB; SFB 638 project A1; DFG-SPP 1132; Promotionskolleg Bioquant; VCI; Boehringer
Ingelheim Fonds; Alexander von Humboldt Foundation; EMBO; industry-support.
PUBLICATIONS (10 selected publications)
Erbse A, Schmidt R, Bornemann T, Schneider-Mergener J, Mogk A, Zahn R, Dougan DA,
Bukau B (2006) ClpS is an essential component of the N-end rule pathway in Escherichia
coli. Nature 439: 753-756.
Vogel M, Bukau B, Mayer MP (2006) Allosteric regulation of Hsp70 chaperones by a proline
switch. Mol Cell 21: 359-367.
Weibezahn J, Tessarz, Schlieker C, Zahn R, Maglica Z, Lee S, Zentgraf H, Weber-Ban E,
Dougan D, Tsai FTF, Mogk A, Bukau B. (2004) Thermotolerance requires refolding of
aggregated proteins by substrate translocation through the central pore of ClpB. Cell 119:
653-665.
Ferbitz L, Maier T, Patzelt H, Bukau B, Deuerling E, Ban N (2004) Structure of the Trigger
Factor chaperone complex with the ribosome defines the molecular environment of the
emerging nascent protein chain. Nature 431: 590-596.
Dougan DA, Weber-Ban E, Bukau B (2003) Targeted delivery of an ssrA-tagged substrate by
the adaptor protein SspB to its cognate AAA+ protein ClpX. Mol Cell 12: 373-380.
Kramer G, Rauch T, Rist W, Vorderwülbecke S, Patzelt H, Schulze-Specking A, Ban N,
Deuerling E, Bukau B (2002) L23 protein functions as a chaperone docking site on the
ribosome. Nature 419: 171-174.
Dougan DA, Reid BG, Horwich AL, Bukau B (2002) ClpS, a substrate modulator of the
ClpAP machine. Mol Cell 9: 673-683.
Brehmer D, Rüdiger S, Gässler CS, Klostermeier D, Packschies L, Reinstein J, Mayer MP,
Bukau B (2001) Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide
exchange. Nat Struct Biol 8: 427-432.
Mayer MP, Schröder H, Rüdiger S, Paal K, Laufen T, Bukau B (2000) Multistep mechanism
of substrate binding determines chaperone activity of Hsp70. Nat Struct Biol 7: 586-953.
Deuerling E, Schulze-Specking A, Tomoyasu T, Mogk A, Bukau B (1999) Trigger factor and
DnaK cooperate in folding of newly synthesized proteins. Nature 400: 693-696

EXPERIENCE IN THE SUPERVISION OF DOCTORAL CANDIDATES
Member of numerous thesis and thesis advisory committees
International reviewer of doctoral theses
Supervised 26 doctoral theses
SUPERVISED DISSERTATIONS (last 5 years)
Holger Raviol, PhD (2006) Functional characterization of the Hsp110 family of molecular
chaperones. University of Heidelberg
Current occupation: Research Scientist, Roche Diagnostics, Mannheim
Jimena Weibezahn, PhD (2006) Investigation of the mechanism of protein disaggregation by
the AAA+ protein ClpB. University of Heidelberg
Current occupation: Postdoctoral fellow, Howard Hughes Medical Institute, UC San
Francisco, USA
Christine Strub, MD (2005) Untersuchungen zur Struktur und Substratspezifität des AAA+-
Chaperons ClpB in Escherichia coli. University of Freiburg
Current occupation: Medical doctor at hospital
Thomas Rauch, PhD (2005) The role of ribosome-associated chaperones in de novo protein
folding. University of Heidelberg, Ruprecht-Karls Prize 2006
Current occupation: Medical Student, University of Heidelberg
Wolfgang Rist, PhD (2004) Mass spectrometric analysis of chaperone-substrate interactions.
University of Heidelberg
Current occupation: Research Scientist, Boehringer Ingelheim Pharma, Biberach
Christian Schlieker, PhD (2004) Substrate recognition and processing by the AAA+
chaperone ClpB. University of Heidelberg, Ruprecht-Karls Prize 2005
Current occupation: Postdoctoral fellow, Harvard Medical School, Boston, USA
Tilman Schlothauer, PhD (2004) Role of the MecA adaptor protein in regulation of AAA+
chaperone ClpC of Bacillus subtilis. University of Heidelberg
Current occupation: Research Scientist, Roche Diagnostics, Penzberg
Sonja Vorderwülbecke, PhD (2003) Struktur-Funktionsanalyse von Trigger Faktor, einem
Ribosomen-assoziierten Chaperon aus Escherichia coli. University of Freiburg
Current occupation: Research Scientist, BioRad, Munich
Christoph Kluck, PhD (2003) Alternative Hsp70-Systeme in Escherichia coli. University of
Heidelberg
Current occupation: Postdoctoral fellow, University of Erlangen-Nürnberg
Holger Patzelt, PhD (2003) Structural and functional characterization of Trigger Factor, a
ribosome-associated chaperone in Escherichia coli. University of Heidelberg,
VAAM Promotion Prize 2004
Current occupation: Senior Research Fellow, Max Planck Institute of Economics,
Jena