Lect. 6 composition & Architecture of plant viruses

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• Positive stains • React chemically with and are bound to virus surface e.g. various Osmium, lead and uranyl compounds and phosphotungustic acid (PTA) are used under appropriate conditions. However, the chemical reaction may alter or disintegrate the virus so –ve stains are more important • Negative stains: Fine structures determination • They do not react with the virus but penetrate available spaces on the surfaces or with in virus particle e.g. Uranylacetate or Potassium phosphotungstate (KPT) are used near pH 5.0
Fine structures determination • Thin sections • Cryo EM • X-ray crystallography analysis • Neutron small angle scattering: • neutron scattering by virus solution is a method by which low resolution information can be obtained about structure <strong>of</strong> virus. E.g. important for radii <strong>of</strong> isometric particles • Mass spectrography • Serological method's • Gel diffusion • ELISA • ISEM
Page 1 and 2: Composition & Architecture of plant
Page 3 and 4: Importance • Detailed knowledge o
Page 5 and 6: Chemical composition of plant virus
Page 7 and 8: Viral Composition • Nucleic acid
Page 9 and 10: %age of protein & n/a in some virus
Page 11 and 12: Watson and Crick • In 1956 propos
Page 13 and 14: Various feature of viruses can be e
Page 15 and 16: • The Transmission Electron Micro
Page 17: Fine structures determination E.M.
Page 21 and 22: Methods for studying stabilizing bo
Page 23 and 24: Architecture of rod shaped viruses
Page 25 and 26: X-ray sources • The brightest and
Page 27 and 28: TMV • TMV particles are: • Rigi
Page 29 and 30: TMV architecture • Negatively sta
Page 31 and 32: Rod shaped particles Helix (rod) e.
Page 33 and 34: Tobacco mosaic virus
Page 35 and 36: Properties of coat proteins • The
Page 37 and 38: Plant viruses are diverse, but not
Page 39 and 40: Icosahedral arrangement is typical
Page 41 and 42: Tobacco necrosis virus, 26 nm in di
Page 43 and 44: Francki, Milne & Hatta. 1985 Atlas
Page 45 and 46: Tobacco mosaic virus (TMV), 300 nm

Lect. 6 composition & Architecture of plant viruses

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