Lect. 6 composition & Architecture of plant viruses

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X-ray crystallography • X-ray crystallography is a method of determining the arrangement of atoms within a crystal, in which a beam of X-rays strikes a crystal and diffracts into many specific directions. • From the angles and intensities of these diffracted beams, a crystallographer can produce a threedimensional picture of the density of electrons within the crystal. • From this electron density we can determined: • the mean positions of the atoms in the crystal, as well as • their chemical bonds, • their disorder and various other information.
X-ray sources • The brightest and most useful X-ray sources are synchrotrons A protein crystal seen under amicroscope. Crystals used in X-ray crystallography may be smaller than a millimeter across. Workflow for solving the structure of a molecule by X- ray crystallography.
Page 1 and 2: Composition & Architecture of plant
Page 3 and 4: Importance • Detailed knowledge o
Page 5 and 6: Chemical composition of plant virus
Page 7 and 8: Viral Composition • Nucleic acid
Page 9 and 10: %age of protein & n/a in some virus
Page 11 and 12: Watson and Crick • In 1956 propos
Page 13 and 14: Various feature of viruses can be e
Page 15 and 16: • The Transmission Electron Micro
Page 17 and 18: Fine structures determination E.M.
Page 19 and 20: Fine structures determination • T
Page 21 and 22: Methods for studying stabilizing bo
Page 23: Architecture of rod shaped viruses
Page 27 and 28: TMV • TMV particles are: • Rigi
Page 29 and 30: TMV architecture • Negatively sta
Page 31 and 32: Rod shaped particles Helix (rod) e.
Page 33 and 34: Tobacco mosaic virus
Page 35 and 36: Properties of coat proteins • The
Page 37 and 38: Plant viruses are diverse, but not
Page 39 and 40: Icosahedral arrangement is typical
Page 41 and 42: Tobacco necrosis virus, 26 nm in di
Page 43 and 44: Francki, Milne & Hatta. 1985 Atlas
Page 45 and 46: Tobacco mosaic virus (TMV), 300 nm

Lect. 6 composition & Architecture of plant viruses

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