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Lect. 6 composition & Architecture of plant viruses

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X-ray crystallography<br />

• X-ray crystallography is a method <strong>of</strong> determining the<br />

arrangement <strong>of</strong> atoms within a crystal, in which a<br />

beam <strong>of</strong> X-rays strikes a crystal and diffracts into<br />

many specific directions.<br />

• From the angles and intensities <strong>of</strong> these diffracted<br />

beams, a crystallographer can produce a threedimensional<br />

picture <strong>of</strong> the density <strong>of</strong> electrons within<br />

the crystal.<br />

• From this electron density we can determined:<br />

• the mean positions <strong>of</strong> the atoms in the crystal, as well as<br />

• their chemical bonds,<br />

• their disorder and various other information.

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