Lect. 6 composition & Architecture of plant viruses
Lect. 6 composition & Architecture of plant viruses
Lect. 6 composition & Architecture of plant viruses
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Methods for studying stabilizing bonds<br />
• The stabilizing interactions are hydrophobic<br />
bonds, H= bonds, salt linkage etc. these<br />
interactions cab be studied by:<br />
• X-ray crystallography<br />
• Stability to chemicals and physical agents: e.g.<br />
Phenol, urea, temperature and detergents etc.<br />
• Chemical modification <strong>of</strong> CP: a/a changes<br />
• Removal <strong>of</strong> ions: in <strong>viruses</strong> whose structure are<br />
stabilized by Ca2+ ions can be affected by their<br />
removal e.g. in isometric particles, CA2+ ions<br />
removal by EDTA causes swelling <strong>of</strong> the particles. So<br />
this phenomenon can give information about the kind<br />
<strong>of</strong> bond important fro virus stability.
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