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Lect. 6 composition & Architecture of plant viruses

Lect. 6 composition & Architecture of plant viruses

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Detailed worked by using<br />

• X-ray defraction gave details <strong>of</strong> arrangement <strong>of</strong><br />

protein subunits and RNA in rod.<br />

• The particles comprises ~2130 subunits that are<br />

closely packed in a helical array.<br />

• The pitch <strong>of</strong> helix is 2.3 (fig.) and the RNA chain is<br />

compactly coiled in a helix following that <strong>of</strong> the<br />

protein subunits<br />

• There are 49 nt. & 161/3 protein subunits per turn<br />

• The PO4 <strong>of</strong> the RNA are at about 4nm from the rod<br />

axis.<br />

• The helix <strong>of</strong> TMV is right handed (Finch, 1972)

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