Abstracts of the Scientific Posters, 2013 AACC Annual Meeting ...

Wednesday, July 31, 9:30 am – 5:00 pm
Lipids/Lipoproteins
CA, USA). Briefly, 25μL of serum sample and 300μL of loading gel were applied to
an 8.0% polyacrylamide gel tube and mixed well. The sample was photopolymerized
at room temperature for 30 minutes and then electrophoresed for 50 minutes (3mA/
gel tube). The sample was then left standing for 30 minutes to prevent dehydration of
the gel and fading of the bands. All of the HDL subfractions were calculated based
on a flotation rate (Rf) between the very low-density lipoprotein (VLDL) fraction and
low-density lipoprotein (LDL) fraction of Rf = 0.0, and the albumin fraction of =1.0.
Subfractions HDL1-3 was defined as large-sized HDL, HDL4-7 as middle-HDL, and
HDL8-10 as small-sized HDL.
Results: After treatment, total cholesterol and LDL-cholesterol levels were
significantly decreased by 21.4% (from 7.14±1.27 to 5.61±0.88 mmol/L, p