Abstracts of the Scientific Posters, 2013 AACC Annual Meeting ...

More documents

Recommendations

Info

Nutrition/Trace Metals/Vitamins Tuesday, July 30, 9:30 am – 5:00 pm 6.5-52.9). Furthermore, 85% and 44% of ED arterogenic and 52% and 37% of ED non-arteriogenic have levels of vitamin D < 30 ng/mL and < 20 ng/mL, respectively, that means an insufficiency and deficiency of vitamin D, respectively. Our study shows that, at least in our experimental conditions, the levels of vitamin D are not sufficiently different to accurately discern between arteriogenic and non-arteriogenic patients and that the deficiency of vitamin D does not characterize only arteriogenic ED but all ED patients. It is probable that in conjunction with other risk factors, the insufficiency/deficiency of vitamin D may be involved in the mechanism causing ED. Conclusion: In conclusion, we hypothesize that a level of vitamin D > 30 ng/mL could prevent the effect of the most common risk factors of ED like atherosclerosis, vascular calcification and endothelial dysfunction. CLINICAL CHEMISTRY, Vol. 59, No. 10, Supplement, 2013 A43
Tuesday, July 30, 9:30 am – 5:00 pm Mass Spectrometry Applications A-155 Tuesday, July 30, 2013 Poster Session: 9:30 AM - 5:00 PM Mass Spectrometry Applications Absolute traceable protein quantification methods using isotope dilution mass spectrometry with three different strategies J. Jeong, Y. Yim, J. Yim, I. Yoon, H. Lee, S. Kim, S. Kim, Y. Lee, S. Park. Korea Research Institute of Standards and Science, Daejeon, Korea, Republic of Background: Measurement traceability in protein quantification is required to standardize quantification results irrespective of the measurement procedure and laboratory. As no analytical method can yet directly quantify whole proteins to the desired level of accuracy, proteins are reduced to analyzable entities with certain stoichiometric values, and which are then analyzed to deduce the quantity of original protein. We described absolute traceable methods in three different strategies based on the peptides, amino acids, and specific element as well. All methods used isotope dilution mass spectrometry to provide traceability to the Système d’Unitè International for quantification of human growth hormone as a candidate of certified reference material. Methods: Purified recombinant human growth hormone (hGH, 22 kDa) was used as a model protein. Sample purity was confirmed using capillary zone electrophoresis and HPLC. First, hGH was hydrolyzed by acid hydrolysis with 8 M hydrochloric acid at 130 ℃ for 48 h for amino acid based quantification. Second, two tryptic peptides from hGH were chosen to determine hGH by tryptic digestion. The target peptides and its isotope residues were synthesized and value assigned by amino acid analysis. Double exact matching ID-HPLC-tandem MS was used for amino acid analysis as well as peptide analysis. Third, hGH, which contains seven sulfur-containing amino acid residues, was reduced into element level with microwave assisted digestion. Digested hGH were determined to total amount of sulfur using ID-inductively coupled plasma/ MS. The results from three different methods were evaluated by comparison with each other. Results: Each method for protein reducing and analytical conditions was optimized. The results from four different amino acids showed good agreement within 2% CV, and also showed excellent intra-day and inter-day precisions of < 1.5% CV. The results from two different peptides agreed with 5% CV with excellent reproducibility (< 2%). At last, the results by sulfur content showed excellent reproducibility within 3% CV. The hGH contents from three different methods were agreed with 5% bias. Sulfurbased result showed a little higher value with smaller uncertainty whereas amino acid-based result showed a lower value, and peptide-based result was in between but showed a little larger uncertainty (5%). Conclusion: The concentration of the hGH was therefore determined based upon the concentration of tryptic peptides, and the concentration of hydrolyzate amino acids, and the concentration of sulfur as well. Although the results were presented small bias, each method is suitable for the accurate quantification of hGH, and could satisfactorily serve as a reference analytical procedure for hGH and other similar proteins. Moreover, these three different methods provide an alternative method for absolute quantification of proteins, especially for pure protein standards. Further researches to resolve small discrepancy among them is in progress. A-156 A Sensitive and Rapid Liquid Chromatography-Tandem Mass Spectrometry Method for Quantification of Lacosamide and Desmethyl Lacosamide using Lacosamide- 13 C,d3 as Internal Standard D. A. Payto, S. Wang. Cleveland Clinic, Cleveland, OH Background: Lacosamide (LCM) is an antiepileptic drug (AED) approved by the FDA in October 2008 for the adjunctive treatment of partial onset seizures. The major metabolite in human is O-desmethyl lacosamide (ODL). Monitoring LCM and ODL may help physicians to optimize the therapeutic dosing. Though there are published methods for the quantification of LCM by either liquid chromatography-ultraviolet (HPLC-UV) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) there are few that measure both LCM and ODL simultaneously and none uses an isotope labeled internal standard (IS) for LC-MS/MS methods. Our objective was to develop and validate a simple, sensitive, and rapid LC-MS/MS assay for the quantification of LCM and ODL using lacosamide- 13 C,d 3 as IS. Methods: Serum (25μL) and 150μL IS solution (5μg/mL of lacosamide- 13 C,d 3 in methanol) were vortex mixed and centrifuged. Supernatant (10μL) was mixed with 1000μL of 0.1% formic acid in water and 3μL was analyzed on an Accucore C18 column in an LC-MS/MS system. Total chromatographic time was 6.5 minutes. A quantifier and a qualifier transition were monitored for LCM (quantifier, 251.1→108.0 and qualifier, 251.1→116.1) and ODL (quantifier, 237.1→108.0 and qualifier, 237.1→91.1). Results: LCM was linear from 0.65 to 44.56 μg/mL with analytical recoveries ranging from 89.7 to 104.8%, while ODL was linear from 0.76 to 48.84 μg/mL with analytical recoveries of 97.8-122.0%. The total coefficient of variation was
Page 1 and 2: Abstracts of the Scientific Posters
Page 3 and 4: Tuesday, July 30, 9:30 am - 5:00 pm
Page 43: Tuesday, July 30, 9:30 am - 5:00 pm
Page 95 and 96:
Tuesday, July 30, 9:30 am - 5:00 pm
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Wednesday, July 31, 9:30 am - 5:00
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Page 267 and 268:
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
Page 277 and 278:
AUTHOR INDEX TO ABSTRACTS OF SCIENT
Page 279 and 280:
Page 281 and 282:
Page 283 and 284:
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
KEYWORD INDEX TO ABSTRACTS OF SCIEN
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
show all

Abstracts of the Scientific Posters, 2013 AACC Annual Meeting ...

Create successful ePaper yourself

Delete template?

Save as template?