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Training Manual Development Of Cultivars And Seed ... - icrisat

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used to establish replicated trials to compare individual selections more critically. Replicated testing of<br />

yield or other traits can begin at any stage after progenies are reasonably uniform.<br />

The criteria used to select plants vary from location to location. Some generally desirable traits<br />

include large panicle, good panicle exertion, appropriate time of maturity, plant height, bold grains, good<br />

seed-set, high seed number, and attractive plant type. Specific criteria, which may differ from location<br />

to location, include resistances to insects and diseases and grain quality for different end-users.<br />

Testing Restoration Ability<br />

Inbred lines with appropriate traits for use as restorer lines must be tested to determine their fertilityrestoration<br />

reaction. Pollen from the test lines is used to pollinate one or more heads of a cytoplasmicgenetic<br />

male-sterile line. The seed produced is harvested and sown to produce a hybrid crop. One or<br />

more heads of the hybrid are bagged prior to flowering to determine whether or not the hybrid is selffertile.<br />

Only lines which give fully fertile hybrids under bagging will be retained as restorers for wide<br />

testing in combination with different male-sterile lines to generate experimental hybrids for agronomic and<br />

yield testing. The fertility of hybrids should be tested during the season in which they will be grown, as<br />

fertility restoration can be influenced by temperature and humidity, and can differ dramatically from<br />

season to season.<br />

Desirable Traits of Good Restorers and Pure-Line Varieties<br />

Restorer lines are normally not intended for direct use as cultivars. Therefore, they do not necessarily<br />

combine all the traits that are favored by farmers. They combine traits that make them suitable for use<br />

as parents of hybrids. These include fertility-restoration reaction, good pollen-shedding capacity,<br />

appropriate height (usually shorter than the varieties), and good grain yield.<br />

The ability of a line to produce good hybrids in combination with male-sterile lines is known as<br />

its combining ability. A line with good general combining ability will tend to give high-yielding hybrids with<br />

many male-sterile parents. Clearly, this is an advantageous trait in a restorer parent. It can be quantified<br />

in comparison with other restorer lines in specially designed testing schemes. In sorghum, combining<br />

ability is closely associated with per se performance of the line. Yield testing of the restorer lines,<br />

therefore, often substitutes for formal combining-ability tests.<br />

Pure-line varieties are intended for use directly by farmers. They must, therefore, combine the<br />

traits desired by the farmers in the target location or area. These normally include high grain yield,<br />

stability of yield over a range of conditions, appropriate duration to maturity, plant height, resistances to<br />

endemic pests and diseases, and suitable grain and stover quality. Typically, pure-line varieties are taller<br />

than restorer lines with higher grain and stover yields.<br />

Maintenance of Restorer Parents and Pure-Line Varieties<br />

The procedures for maintenance of restorer lines and pure-line varieties of sorghum are essentially the<br />

same. In both cases, the process begins with planning seed requirements and projecting backwards.<br />

Using conservative estimates of yields likely to be achieved, the size of the maintenance exercise<br />

required at each stage and its timing to ensure prompt delivery of the final product is planned.<br />

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