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Molecular Breeding in Minor Oilseed Crops: Opportunities ... - icrisat

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Use of SNP – characterization of <strong>in</strong>bred l<strong>in</strong>es<br />

(Fusari et al., 2008, BMC Plant Biology 8:7)<br />

• A set of 28 candidate genes related to biotic and abiotic stresses<br />

studied <strong>in</strong> 19 sunflower <strong>in</strong>bred l<strong>in</strong>es.<br />

• A total of 14,348 bp of sequence alignment analyzed per <strong>in</strong>dividual (1<br />

SNP/ 69 nucleotides and 38 <strong>in</strong>dels identified)<br />

• The number of haplotypes per region ranged from 1 to 9<br />

(mean = 3.54 ± 1.88).<br />

• Two putative gene pools identified (G1 and G2), with a large<br />

proportion of the <strong>in</strong>bred l<strong>in</strong>es be<strong>in</strong>g assigned to G1<br />

• LD for G1 decayed more rapidly (r2 = 0.48 at 643 bp) than the LD<br />

trend l<strong>in</strong>e for the entire set of 19 <strong>in</strong>dividuals<br />

(r2 = 0.64 for the same distance)<br />

This work could facilitate association mapp<strong>in</strong>g with lower marker<br />

densities than those usually reported for other plant species

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