MAKETA 5/2 po
MAKETA 5/2 po
MAKETA 5/2 po
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A C T A M E D I C A M A R T I N I A N A 2 0 0 5 5/2 5<br />
ing to the calibration curve and expressed as a percentage of the total amount administered to<br />
an individual animal.<br />
Estimation of meconium removal by meconium-crit method<br />
Basic suspension of 3.0 g of meconium in 100 ml of normal saline was diluted to seven different<br />
concentrations (30, 20, 15, 10, 6, 3, and 2 mg/ml). Ten samples of each suspension were<br />
taken into the standard microhematocrit glass tubes and centrifuged at 10 000 rpm for 5 minutes<br />
(11). The meconium-crit was then measured directly as the hematocrit, i.e. as a ratio of the<br />
solid content to the total volume, and mean values were calculated. Calibration curve showing<br />
the relationship between the concentration of meconium and meconium-crit value was constructed<br />
(Fig. 2). In experiments, three samples of the lavage fluid were taken into the microhematocrit<br />
glass tubes and centrifuged at 10 000 rpm for 5 min. Percentage of solid content was<br />
read. Average value of removed meconium was expressed as a percentage of the total amount<br />
administered to an individual animal.<br />
Fig. 2. Calibration curve for meconium-crit method.<br />
STATISTICS<br />
Between-group differences were analysed using Mann-Whitney’s test. A P-value0.05). Animals of both groups were given comparable amount of<br />
meconium – 100 mg/kg b.w. (25 mg/ml, 4 ml/kg), i.e. total mean value 110.7±13.4 mg of meconium<br />
per kg b.w.<br />
As shown by spectrophotometry, amount of meconium removed by saline lavage was<br />
12.7±2.0 mg/kg b.w., i.e. 11.0±1.1 % of administered meconium, while by surfactant lung lavage<br />
it was 15.1±3.1 mg/kg b.w., i.e. 13.6±2.5 % of administered meconium. By meconium-crit<br />
method, saline lavage removed 7.9±2.8 mg/kg b.w. of meconium, i.e. 5.4±2.2 % of instilled meconium,<br />
and surfactant lavage removed 19.1±9.7 mg/kg b.w., i.e. 11.1±4.5 % of administered<br />
meconium. Statistical between-group comparison revealed that amount of meconium removed<br />
by surfactant lung lavage compared to saline lavage was higher, when evaluated by meconiumcrit<br />
method than by spectrophotometry (Fig. 3, Fig. 4).