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Roscoff Culture Collection - Station Biologique de Roscoff - Portail ...

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<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />

mardi 3 juillet 2001<br />

RCC 141<br />

Class<br />

Strain EUM16BBL Sub Strain:<br />

Other Name<br />

Derived from:<br />

<strong>Culture</strong> isolation<br />

<strong>Station</strong>: OLIGO<br />

Temperature (°C):<br />

<strong>Culture</strong> conditions initally<br />

Remarks<br />

CTD:<br />

<strong>Culture</strong> conditions <strong>Roscoff</strong><br />

<strong>Culture</strong> purification<br />

axenisation<br />

<strong>Culture</strong> data<br />

Sent to Oslo<br />

Prasinophyceae<br />

Medium SBR:<br />

K<br />

Mixed<br />

Oceanic origin Tropical Atlantic Cruise: EUMELI 3<br />

Bottle:<br />

Clonal<br />

Size 1.5<br />

Depth: 105<br />

Date: 01/10/91<br />

Isolator<br />

Latitu<strong>de</strong>: 21 ° 2 ' Longitu<strong>de</strong> : 31 ° 8 '<br />

Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />

Temperature SBR (°C): 20 Light SBR (uE): 4<br />

04/01. Sequence confirms it is very close to Ostreococcus oceanicus.<br />

Wenche 03/03. This is not Bathycoccus.<br />

DV jan 01. Very small coccoids almost black in PC. Ostreoccoccus? Make small clumps.<br />

09/2000, Florence: Coccoï<strong>de</strong>s <strong>de</strong>nses (1à2µm)<br />

Partensky<br />

Transfer period SBR (weeks) 2 Tube SBR: 21 Etiquettes: group A<br />

Purification date: Purified by: Purification method:<br />

I<strong>de</strong>ntification priority: 1<br />

Axenic<br />

Genus Ostreococcus<br />

Frozen<br />

Pigment analysis<br />

Toxic<br />

Species sp<br />

Heterotrophic<br />

Date entered catalog: 01/11/98<br />

Publish to web catalog<br />

Lost<br />

Date Lost:<br />

Backup for: CCMP number:<br />

I<strong>de</strong>ntified by: Owned by: SBR_Phyto<br />

Medium: mod. K/10<br />

Full study priority: 1<br />

Category<br />

Light (uE): 10<br />

Pico<br />

Method:<br />

Room SBR: 140<br />

Pigment phenotype (for cyanos) :<br />

W<br />

SSU rRNA and RFLP pattern<br />

ZZ006608 Prasinophyceae Ostreococcus oceanicus<br />

RC 141 Clone #<br />

Closest species<br />

Enzyme HaeIII Remarks<br />

556 377 295 260 177<br />

I<strong>de</strong>ntical to RCC<br />

RC Clone # Closest species Rhaphoneis belgicase (diatom)<br />

Mixed<br />

Flow cytometry<br />

RCC<br />

141 Population number 1<br />

01/12/00 File RCC141<br />

Desc.<br />

RCC141 fluo nat<br />

Bead size 0.95<br />

Cell_ml 12691<br />

Setttings Values<br />

FSC E02 1.06<br />

SSC 400 0.33<br />

FL1<br />

FL2<br />

FL3 600<br />

0.00<br />

0.00<br />

13.98<br />

Remark<br />

<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />

Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.

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