Regulation of gene expression in eukaryotes
Regulation of gene expression in eukaryotes
Regulation of gene expression in eukaryotes
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B. Eukaryotic Promoters<br />
Usually located with<strong>in</strong> 100 bp upstream<br />
‣ Recognized byRNA Pol II (transcribes mRNA)<br />
‣ Require the b<strong>in</strong>d<strong>in</strong>g <strong>of</strong> several prote<strong>in</strong> factors to <strong>in</strong>itiate<br />
transcription (DNA b<strong>in</strong>d<strong>in</strong>g doma<strong>in</strong>s on TFs – ‘motifs’)<br />
‣ May be positively or negatively regulated<br />
C. Transcription Factors –the<br />
transcription complex<br />
1) TFIIA, TFIIB,<br />
TFIID, TFIIE,<br />
TFIIH<br />
2) TATA b<strong>in</strong>d<strong>in</strong>g<br />
prote<strong>in</strong> (TBP)<br />
3) TBP associated<br />
factors (TAFs)<br />
‣Assembly <strong>of</strong> the basal<br />
transcription apparatus -<br />
<strong>in</strong>volves stepwise b<strong>in</strong>d<strong>in</strong>g<br />
<strong>of</strong> various transcription<br />
factor prote<strong>in</strong>s.<br />
Commitment Stage &<br />
Clearance Stage…<br />
3
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