5 References 5 References Abbott, K.L., Matthews, R.T. & Pierce, M. (2008) Receptor tyrosine phosphatase beta (RPTPbeta) activity and signaling are attenuated by glycosylation and subsequent cell surface galectin-1 binding. J Biol Chem, 283, 33026-33035. Akasaka-Manya, K., Manya, H., Nakajima, A., Kawakita, M. & Endo, T. (2006) Physical and functional association of human protein O-mannosyltransferases 1 and 2. J Biol Chem, 281, 19339-19345. Apweiler, R., Hermjakob, H. & Sharon, N. (1999) On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochim Biophys Acta, 1473, 4-8. Aspberg, A., Miura, R., Bourdoulous, S., Shimonaka, M., Heinegârd, D., Schachner, M., Ruoslahti, E. & Yamaguchi, Y. (1997) The C-type lectin domains of lecticans, a family of aggregating chondroitin sulfate proteoglycans, bind tenascin-R by protein-protein interactions independent of carbohydrate moiety. Proc Natl Acad Sci U S A, 94, 10116-10121. Bandtlow, C.E. & Zimmermann, D.R. (2000) Proteoglycans in the developing brain: new conceptual insights for old proteins. Physiol Rev, 80, 1267-1290. Barone, R., Aiello, C., Race, V., Morava, E., Foulquier, F., Riemersma, M., Passarelli, C., Concolino, D., Carella, M., Santorelli, F., Vleugels, W., Mercuri, E., Garozzo, D., Sturiale, L., Messina, S., Jaeken, J., Fiumara, A., Wevers, R.A., Bertini, E., Matthijs, G. & Lefeber, D.J. (2012) DPM2-CDG: a muscular dystrophy-dystroglycanopathy syndrome with severe epilepsy. Ann Neurol, 72, 550-558. Barresi, R. & Campbell, K.P. (2006) Dystroglycan: from biosynthesis to pathogenesis of human disease. J Cell Sci, 119, 199-207. Bartus, K., James, N.D., Bosch, K.D. & Bradbury, E.J. (2012) Chondroitin sulphate proteoglycans: key modulators of spinal cord and brain plasticity. Exp Neurol, 235, 5-17. Bekku, Y., Vargová, L., Goto, Y., Vorísek, I., Dmytrenko, L., Narasaki, M., Ohtsuka, A., Fässler, R., Ninomiya, Y., Syková, E. & Oohashi, T. (2010) Bral1: its role in diffusion barrier formation and conduction velocity in the CNS. J Neurosci, 30, 3113-3123. Beltrán-Valero de Bernabé, D., Currier, S., Steinbrecher, A., Celli, J., van Beusekom, E., van der Zwaag, B., Kayserili, H., Merlini, L., Chitayat, D., Dobyns, W.B., Cormand, B., Lehesjoki, A.E., Cruces, J., Voit, T., Walsh, C.A., van Bokhoven, H. & Brunner, H.G. (2002) Mutations in the O-mannosyltransferase gene POMT1 give rise to the severe neuronal migration disorder Walker-Warburg syndrome. Am J Hum Genet, 71, 1033-1043. 61
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Identification and Characterization
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I Index I Index of Contents I INDEX
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I Index FIGURE 13: WESTERN BLOT OF
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I Index I.III List of Abbreviations
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I Index PBS PMT PNN Phosphate Buffe
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I Index I.III.II Amino acids - thre
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I Index II Zusammenfassung Die O-Ma
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1 Introduction glycosidically linke
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1 Introduction glycans, complex-typ
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1 Introduction O-glycosylation (typ
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1 Introduction Figure 3: Schematic
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- Page 25 and 26: 1 Introduction posttranslational pr
- Page 27 and 28: 1 Introduction transfer of mannose
- Page 29 and 30: 1 Introduction Together the above m
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- Page 33 and 34: 2 Materials and methods Lysis and W
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- Page 41 and 42: Figure 9: O-Glycome of mouse brain.
- Page 43 and 44: 3 Results Table 1: Sequential extra
- Page 45 and 46: 3 Results that this protein might b
- Page 47 and 48: 3 Results 3.2 O-Mannosylation on ne
- Page 49 and 50: 3 Results identified by several 361
- Page 51 and 52: 3 Results Figure 15: Fragmentation
- Page 53 and 54: 3 Results Table 2: Protein identifi
- Page 55 and 56: 3 Results Three other O-mannosyl gl
- Page 57 and 58: 3 Results 3.3 O-Mannosylation of le
- Page 59 and 60: 3 Results 3.3.2 Recombinant express
- Page 61 and 62: 3 Results identified just as the gl
- Page 63 and 64: 3 Results Subsequentially, the resu
- Page 65 and 66: 3 Results In order to get more info
- Page 67 and 68: 3 Results brain-specific protein ne
- Page 69 and 70: 4 Summary and outlook malformations
- Page 71 and 72: 4 Summary and outlook O-mannosylati
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- Page 77 and 78: 5 References Frischknecht, R. & Sei
- Page 79 and 80: 5 References Kriebel, M., Metzger,
- Page 81 and 82: 5 References Nizet, V. & Esko, J. (
- Page 83 and 84: 5 References Susuki, K. & Rasband,
- Page 85 and 86: 6 Appendix 6 Appendix Table 6: Summ
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- Page 95 and 96: 6 Appendix Figure 38: Analysis of t
- Page 97 and 98: 6 Appendix Figure 40: Calf brain-de
- Page 99 and 100: 6 Appendix Table 13: Protein identi
- Page 101 and 102: 6 Appendix Table 14: Summary of hum
- Page 103: Erklärung Ich versichere, dass ich