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Hixon 1 Concentrations and identities of fecal coliform bacteria from ...

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<strong>Hixon</strong> 2<br />

ABSTRACT<br />

Over the centuries humans have impacted many streams <strong>and</strong> rivers in Florida causing<br />

many <strong>of</strong> them to become polluted. Fecal <strong>coliform</strong> <strong>bacteria</strong>, which live in the guts <strong>of</strong> warm<br />

blooded animals, are common in polluted streams <strong>and</strong> rivers <strong>and</strong> are currently used as indicators<br />

<strong>of</strong> the presence <strong>of</strong> human pathogenic microorganisms. There are over 100 tributarites that flow<br />

into the Saint Johns River in Jacksonville Florida that are monitored for <strong>fecal</strong> <strong>coliform</strong> <strong>bacteria</strong>.<br />

According to the Florida Department <strong>of</strong> Environmental Protection (FDEP) if there are more than<br />

800 <strong>fecal</strong> <strong>coliform</strong> colony forming units (CFUs) per 100mL, the water is deemed not safe for<br />

human recreation. This paper focuses on descriptive <strong>and</strong> comparative research at six sites along<br />

the Saint Johns River near Jacksonville University (JU). The <strong>fecal</strong> <strong>coliform</strong> <strong>bacteria</strong> ranged <strong>from</strong><br />

1 to 1067 CFUs/100mL, with the highest counts at Jacksonville University. There were no<br />

apparent relationships seen between <strong>fecal</strong> <strong>coliform</strong> <strong>bacteria</strong> CFUs <strong>and</strong> water temperature,<br />

dissolved oxygen, salinity, <strong>and</strong> pH. Common <strong>fecal</strong> <strong>coliform</strong> <strong>bacteria</strong> belonging to the genera<br />

Escherichia, Klebsiella, <strong>and</strong> Citrobacter were identified.<br />

INTRODUCTION<br />

Water quality is a serious issue that affects many U.S. streams <strong>and</strong> rivers, including the<br />

Saint Johns River in Jacksonville Florida. According to a report by Lui et al. (2010) that assessed<br />

39% <strong>of</strong> the United States streams <strong>and</strong> rivers, 19% were found to be impaired, <strong>of</strong> which 35%<br />

percent were polluted by <strong>fecal</strong> <strong>coliform</strong> <strong>bacteria</strong>. Fecal <strong>coliform</strong> <strong>bacteria</strong> are listed as the leading<br />

causes <strong>of</strong> water quality impairment in rivers <strong>and</strong> streams <strong>and</strong> the second leading cause in<br />

estuaries (Liu et al. 2010).

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