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Hixon 1 Concentrations and identities of fecal coliform bacteria from ...

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<strong>Hixon</strong> 9<br />

All samples were collected around low tide in the Saint Johns River. The first collection<br />

<strong>of</strong> water samples were completed on January 29 th 2011 <strong>from</strong> 11:48 to 15:20 <strong>and</strong> the second<br />

collection <strong>of</strong> water samples were completed on March 12 th 2011 <strong>from</strong> 6:30 to 8:30. Low tide for<br />

the 1/29/11 collection was at 12:59 <strong>and</strong> 9:15 for the 3/12/11 collection. The outside air<br />

temperature for the 1/29/11 collection was 17.8ºC <strong>and</strong> 6.7ºC for the 3/12/11 collection. The<br />

average <strong>fecal</strong> <strong>coliform</strong> <strong>bacteria</strong> concentrations for the 1/29/11 collection ranged <strong>from</strong> 34 to 1067<br />

CFUs/100mL, with JU1 being the highest. For the 3/12/11 collection, the average <strong>fecal</strong> <strong>coliform</strong><br />

<strong>bacteria</strong> concentrations was much less than 1/29/11 <strong>and</strong> ranged <strong>from</strong> 1 to 124 CFUs/100mL, with<br />

LB1 being the highest <strong>and</strong> JU1 being the second highest (99 CFUs/100mL). Site JU1 enters the<br />

Saint Johns River <strong>from</strong> a pipe that goes under the road leading to the Jacksonville University’s<br />

boat house. Based on numerous visual observations, the water coming out <strong>of</strong> this pipe is assumed<br />

to be constantly flowing. After tracking the stream it was found that it is short, going through the<br />

woods <strong>and</strong> eventually drying up upstream before approaching a road on main campus. It is<br />

believed the water in this stream is primarily <strong>from</strong> run<strong>of</strong>f <strong>from</strong> the densely wooded area the<br />

stream travels through. The root cause <strong>of</strong> the high <strong>fecal</strong> <strong>coliform</strong> CFUs has not been proven; but<br />

it is assumed that it is due to <strong>fecal</strong> material <strong>from</strong> animals running <strong>of</strong>f into the stream. JU2 had the<br />

lowest <strong>fecal</strong> <strong>coliform</strong> CFUs at both collections, which was due to the site being the only sampled<br />

location that was in the St. Johns River <strong>and</strong> not a tributary; it is expected that the <strong>fecal</strong> <strong>coliform</strong><br />

<strong>bacteria</strong> concentration is diluted in the river. Fecal <strong>coliform</strong> <strong>bacteria</strong> concentrations for the<br />

1/29/11 collection were more consistent with the City <strong>of</strong> Jacksonville (COJ) data than the<br />

3/12/11 collection. Comparing 1/29/11 <strong>and</strong> 3/12/11 <strong>fecal</strong> <strong>coliform</strong> <strong>bacteria</strong> CFUs data with COJ<br />

data <strong>from</strong> February 2009, the differences for the 1/29/11 collection ranged <strong>from</strong> 20 to 460 CFUs,<br />

with LB1 having the largest difference, <strong>and</strong> differences for the 3/12/11 collection ranged <strong>from</strong>

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