Resassol PG-6 Coconut Marketing Sheet - Kinetik

% Inhibition
IN VITRO TEST
The toxicity of surfactants is determined by their capacity to degrade lipids, as the membrane of eukaryotic cells is mainly
composed of lipids they are particularly sensitive to their “degreasing” action.
The permeabilization of the membranes deriving from the use of surfactants determines, initially, the diffusion to the outside
of some cytoplasmic solutes among which the Lactate Dehydrogenase enzyme (LDH) and, subsequently, the death of the
cells.
(The release of LDH in the culture medium is considered an early marker of toxicity because it is possible to find it at the
beginning of the lesion several hours before the death of the cells).
The test was performed on human keratinocytes which represent the first barrier against external aggression, and which
are therefore the first to counter the effects of the surface-acting agents also In Vivo. The cells were exposed to contact
with a solution containing RESASSOL ® PG6 COCONUT at 0.04% and SLES at 0.003% for periods of 60-120 and 180
minutes.
At each interval an assay of the LDH released was carried out, the internal check is represented by an identical solution
of SLES 0.003% containing 0.01% of Betaine, the positive check is represented by the solution of SLES at 0.003%.
At the end of the last incubation of 180 minutes the treatments were removed and replaced with normal culture medium
for a recovery period of 22-24 hours (to check whether the lesions inflicted by the treatment were capable of causing the
death of the cells or not: in other words in this way we also checked the seriousness of the lesions suffered by the cells
regardless of the quantity of LDH released).
PERMEABILIZATION OF MEMBRANES
INHIBITION RELEASE OF LDH
RESASSOL PG6 COCONUT
The results show how the presence of the coconut and
polyglycerol derivate is capable of increasing the dermocompatibility
of the SLES solution; actually, it stops the
release of LDH within the first 120 minutes of exposure and
it reduces it by 93% after 180 minutes. The comparison
with Betaine brings out even more the effectiveness of the
product; indeed, even though it too is capable of reducing
considerably the release of the enzyme after 60 and 120
minutes of treatment, after 180 minutes its action is limited
to inhibiting release of LDH only by 48%.
The results obtained reveal an excellent detoxifying
action of the coconut and polyglycerol derivates with
the SLES solution and therefore a strong capability
of the product to increase the dermo-compatibility
of primary surfactants.
RESASSOL PG6 COCONUT
% VITALITY
RESASSOL PG6 COCONUT
POST RECOVERY VITALITY
The results obtained reveal how both polyglycerols and
betaine have reduced the toxicity of the SLES solution:
Indeed, both allowed a survival level around 100%, while
the cells exposed to SLES only show a vitality of 27%.
Polyglycerols have shown to have a better detoxifying action
considering that, against a survival rate practically equal
to that of betaine, it determines a definitely lower release
of LDH at 180 minutes. The LDH is only one of the proinflammatory
mediators that are released during the
inflammatory reactions typical of an irritation, which can
appear also in the absence of cell death and/or necrosis;
consequently we can say that polyglycerols have a
higher dermo-compatibility and detoxifying action
than betaine has.
RESASSOL
% VITALITY