Resassol PG-6 Coconut Marketing Sheet - Kinetik

RESASSOL ® PG6 COCONUT
Mild surfactant from Coconut oil
RESASSOL ® PG6 COCONUT is the first derivative from
the new “Green-Cosmetic” project, financed by the
Lombardy Region (Priority Thematic Areas 2009, POR
2007-2013, Objective Regional competitiveness and
occupation).
Res Pharma Industriale has developed new raw
materials for cosmetics through the transesterification of
polyglycerols 6 from vegetables oils, namely coconut oil
derivates to be used as secondary surfactants and from
olive, rice and almond derivates to be used in the field
of emulsions.
The project has involved Res Pharma Industriale, two
small-medium sized companies, a multinational company
and the public body ISMAC CNR for two years (2010-
2012) with the object of developing by means of green
chemistry new streamlined polymer materials from natural
raw materials: vegetable oils, polyglycerols, up to the
realization of innovative cosmetic products through new
materials and processes having a low environmental
impact and significant energy savings.
INCI: Coconut oil polyglyceryl -6 esters
New secondary PEG FREE surfactant
based on polyglycerine 6 and coconut oil.

% Inhibition
IN VITRO TEST
The toxicity of surfactants is determined by their capacity to degrade lipids, as the membrane of eukaryotic cells is mainly
composed of lipids they are particularly sensitive to their “degreasing” action.
The permeabilization of the membranes deriving from the use of surfactants determines, initially, the diffusion to the outside
of some cytoplasmic solutes among which the Lactate Dehydrogenase enzyme (LDH) and, subsequently, the death of the
cells.
(The release of LDH in the culture medium is considered an early marker of toxicity because it is possible to find it at the
beginning of the lesion several hours before the death of the cells).
The test was performed on human keratinocytes which represent the first barrier against external aggression, and which
are therefore the first to counter the effects of the surface-acting agents also In Vivo. The cells were exposed to contact
with a solution containing RESASSOL ® PG6 COCONUT at 0.04% and SLES at 0.003% for periods of 60-120 and 180
minutes.
At each interval an assay of the LDH released was carried out, the internal check is represented by an identical solution
of SLES 0.003% containing 0.01% of Betaine, the positive check is represented by the solution of SLES at 0.003%.
At the end of the last incubation of 180 minutes the treatments were removed and replaced with normal culture medium
for a recovery period of 22-24 hours (to check whether the lesions inflicted by the treatment were capable of causing the
death of the cells or not: in other words in this way we also checked the seriousness of the lesions suffered by the cells
regardless of the quantity of LDH released).
PERMEABILIZATION OF MEMBRANES
INHIBITION RELEASE OF LDH
RESASSOL PG6 COCONUT
The results show how the presence of the coconut and
polyglycerol derivate is capable of increasing the dermocompatibility
of the SLES solution; actually, it stops the
release of LDH within the first 120 minutes of exposure and
it reduces it by 93% after 180 minutes. The comparison
with Betaine brings out even more the effectiveness of the
product; indeed, even though it too is capable of reducing
considerably the release of the enzyme after 60 and 120
minutes of treatment, after 180 minutes its action is limited
to inhibiting release of LDH only by 48%.
The results obtained reveal an excellent detoxifying
action of the coconut and polyglycerol derivates with
the SLES solution and therefore a strong capability
of the product to increase the dermo-compatibility
of primary surfactants.
RESASSOL PG6 COCONUT
% VITALITY
RESASSOL PG6 COCONUT
POST RECOVERY VITALITY
The results obtained reveal how both polyglycerols and
betaine have reduced the toxicity of the SLES solution:
Indeed, both allowed a survival level around 100%, while
the cells exposed to SLES only show a vitality of 27%.
Polyglycerols have shown to have a better detoxifying action
considering that, against a survival rate practically equal
to that of betaine, it determines a definitely lower release
of LDH at 180 minutes. The LDH is only one of the proinflammatory
mediators that are released during the
inflammatory reactions typical of an irritation, which can
appear also in the absence of cell death and/or necrosis;
consequently we can say that polyglycerols have a
higher dermo-compatibility and detoxifying action
than betaine has.
RESASSOL
% VITALITY

PHOTOGRAPHS AFTER RECOVERY (MTT)
Pictures show an high mortality of cells
exposed to the SLES solution ( Image 1 ).
On the contrary, the % of survival remarkably
increases for cells exposed to the solution
RESASSOL ® PG6 COCONUT+SLES (
Image 4 ) and BETAINE+SLES ( Image 3).
Notwithstanding both samples set to zero
the % of cell mortality ( vitality experiment),
cells treated with BETAINE+SLES are more
suffering even if alive.
Fig.1: Negative check (4x) Fig.2: RESASSOL® PG6 COCONUT 0.04%
+SLES 0.003% (4x)
These results confirm the results of the
permeabilization of membranes: treatment
with BETAINE does not set to zero the
release of LDH at 180 minutes of exposition;
BETAINE cannot buffer with the same
efficiency the solution of SLES which,
damaging the membranes, compromise the
wellness of treated cells.
RESASSOL ® PG6 COCONUT gives the
best result in terms of increasing the
dermo-compatibility of the SLES
solution.
Fig.3: Betaine 0.01%+SLES 0.003% (4x)
Fig.4: Positive check, SLES 0.003% (4x)
APPLICATION STUDIES
From studies performed both on SLES and SLES FREE surfactant systems, we are able to say that RESASSOL ® PG6 COCONUT
increases foam height and persistence, enriching the formulation with the emollient properties deriving from the starting oil.
FOAM HEIGHT
Foam height of a mixture containing increasing concentration of RESASSOL ® PG6 COCONUT in solution with different primary
surfactants has been evaluated during the time.
+RESASSOL PG6 COCONUT 3%
+RESASSOL PG6 COCONUT 6%
+RESASSOL PG6 COCONUT 3%
+RESASSOL PG6 COCONUT 6%
+RESASSOL PG6 COCONUT 3%
+RESASSOL PG6 COCONUT 6%
In blends with SLES, the RESASSOL ® PG6 COCONUT shows that it improves the foam height with concentrations
above 6%. In particular, it keeps consistency and permanence in time. It was also added to other non
ethoxylated primary surfactants, confirming its capacity to increase and stabilize foam.

With these last surfactants, its effectiveness is revealed at lower
concentrations compared to the combinations with SLES ( 3%).
RESASSOL PG6 COCONUT Vs Betaine
The graph here below even shows that when associated with
the Caprylyl/Capryl Glucoside system it is more efficient as a
foam stabilizer than the betaine system.
RESASSOL PG6 COCONUT 3%
200ml demineralized water is added to 1% of surfactant mixtures.
10 Pistons hits - diameter 5.5 cm - number of holes: 26 (diameter 0.4 cm)
APPLICATIONS
Recommended for mild formulations, specific for different
ages (baby, senior) and applications on delicate areas
(Intimate hygiene, products for scalp).
TOXICOLOGICAL DATA
Test in Vitro Ocular Irritection ® : Mild irritant
Test in Vitro Dermal Irritection ® : Not irritant
70400_eng
The data provided in this publication is based on our current
knowledge and experience and is offered free of charge and
in good faith for guidance only. It does not constitute a
guarantee in the legal sense of the term and in view of the
multiple factors that may affect processing and applications,
it does not relieve those to whom we supply our products
from the responsibility of carrying out their own tests and
experiments. All express and implied warranties are disclaimed.
Any relevant patent rights and existing legislations and
regulations must be observed.
www.respharma.com
Res Pharma s.r.l.
Via Giulio Pastore, 3 - Trezzo sull'Adda (MI) Italia
Tel. 02.909941 - Fax. 02.90963944
E-mail: info@respharma.com
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