Becker & Hickl GmbH

Becker & Hickl GmbH 

Technology Leader in Photon Counting 

2013

About bh 


Founded in 1993, Becker & Hickl have introduced a proprietary time-correlated single-photon counting principle that made TCSPC more 

than 100 times faster than the existing devices. Moreover, bh introduced a multi-dimensional TCSPC process that records the photons not 

only versus the time in the signal period, but also versus other parameters, such as experiment time, wavelength, or spatial coordinates. The 

bh devices are designed to record multi-dimensional photon distributions, time-resolved images, sequences of photon distributions, or multidimensional 

time-tag data. The bh TCSPC products are complemented by bh picosecond diode lasers, detector modules, multi-spectral 

detector assemblies, and experiment control modules. Based on these components Becker & Hickl supply their own confocal fluorescence 

lifetime laser scanning microscope and FLIM upgrade kits for laser scanning microscopes of various manufacturers. Moreover, bh is supplier 

of TCSPC modules for commercial time-domain optical tomography instruments. bh market activities include currently 5 workshops around 

the world yearly and the distribution of more than 1200 pages of TCSPC literature. 

1993 Introduction of multidimensional TCSPC. Introduction of fast TAC/ADC conversion. TCSPC becomes 100 times faster than it was before. SPC- 

300 modules with 32 or 128 simultaneously recording detector channels. Time channel width 820 fs. Saturated count rate 3 MHz. 

1994 SPC-330 modules. Improved IRF width. 

Routers for parallel operation of detectors. 

1995 SPC-430 modules. Continuous Flow mode for infinite sequential recording. Saturated count rate 8 MHz. First single-molecule applications. 

Resolution of bursts, lifetime analysis within bursts. 

1996 SPC-432 modules. Introduction of Time-Tag recording for single molecule spectroscopy. 

First version of PML-16 sixteen channel PMT module 

1996 SPC-535 modules. First implementation of TCSPC FLIM. First FLIM applications. 

PMS-300 gated photon counter and multichannel scaler 

1997 SPC-630 modules. Introduction of PCI bus. Modules contain time-tag mode, standard photon distribution modes, and continuous flow mode. 

PMH-100 detector module simplifies system setup. 

1998 SPC-730 modules. Introduction of TCSPC FLIM. Scan Sync In and Scan Sync out mode. First applications in FLIM microscopy. 

MSA-300 multichannel scaler. 100 MHz count rate, 5 ns channel width. 

1999 SPC-134 four-channel packages for optical tomography. Devices contain continuous flow mode, standard photon distribution modes, and time-tag 

mode. Saturated sustained count rate 32 MHz. 

SPC-130 TCSPC board for industrial applications. 

2000 FLIM upgrade kits for Zeiss LSM 510 NLO microscopes. 

SPCImage FLIM data analysis software. 

MSA-1000 multichannel scaler. 1 ns resolution, count rate 1000 MHz. 

BHL-600 red and NIR picosecond diode lasers. 

New SPCM software introduces autosave, auto-accumulate, and automatic page stepping functions 

2001 SPC-830 modules. Fast DMA bus interface. Saturated sustained count rate 8 MHz. Standard photon distribution modes, FLIM modes, time-tag 

recording. For the first time, FLIM and single-molecule techniques were combined in one instrument. 

DCC-100 detector controller solves the problem of detector overload. 

PMC-100 cooled detector modules. Internal preamplifier, internal high-voltage generator, overload shutdown. System setup is greatly simplified. 

Detector-Shutter modules for non-descanned ports of multiphoton microscopes. 

2002 FLIM systems for Leica, Olympus, and Biorad laser scanning microscopes. 

First multi-spectral FLIM 

BHLP-700 NIR picosecond diode lasers. Up to 10 mW at 785 nm. 

BDL-405 UV picosecond diode lasers. 

DDG-100 pulse generator for multiplexed operation of lasers and SPC modules. 

First demonstration of double-exponential FRET imaging. 

2003 SPC-144 four-channel TCSPC Imaging Package. Saturated sustained count rate 40 MHz. 

BDL-375, 440, and 473 picosecond diode lasers 

PML-16 multi-wavelength detector 

Online FCS, PCH, and MCS trace calculation in the time-tag mode of the bh TCSPC modules 

2004 Simple-Tau 140 and Simple-Tau 830 compact TCSPC systems. 

MW-FLIM, first multi-spectral NDD FLIM systems for multiphoton microscopes. 

Macro-time synchronisation of several TCSPC modules. First full correlation down to the picosecond region. 

2005 New PML-16 sixteen channel detector. Internal high-voltage generator, overload shutdown, control via DCC-100. 

High-efficiency single-mode fibre coupling of BDL picosecond diode lasers 

FLIM upgrade kits for confocal laser scanning microscopes based on bh BDL-SM lasers and bh TCSPC technology. 

W. Becker, Advanced Time-Correlated Single Photon Counting Techniques published by Springer. 

First edition of BH TCSPC Handbook. 360 pages, 364 references. 

2006 High-power CW mode for BDL-SMC picosecond diode lasers. 

Second edition of bh TCSPC Handbook. 387 pages, 387 references. 

2007 DCS-120 confocal laser scanning systems. Complete fluorescence-lifetime laser scanning microscopes. 

DPC-230 16 channel photon correlator, 160 ps resolution. 

SPC-154 package and SPC-150 modules. Standard photon distribution modes, continuous flow mode, TCSPC imaging, continuous-flow imaging, 

imaging in the time-tag mode. Imaging in time-tag mode for SPC-140 and SPC-830. 

2008 FLIM systems for Zeiss LSM 710 microscopes. NDD FLIM systems for Leica SP2 MP and SP5 MP microscopes. Simple-Tau 152 and -154 two 

and four channel TCSPC systems. 3rd edition of bh TCSPC Handbook. 

2009 GaAsP hybrid detectors. 8-channel parallel TCSPC and TCSPC FLIM systems 

2010 Eight-channel SPAD detector. GaAsP hybrid detectors in all bh FLIM systems. Z stack FLIM. 4th edition of bh TCSPC Handbook 

2011 Phosphorescence Lifetime imaging (PLIM) in DCS-120 and Zeiss LSM 710 FLIM systems. Fluorescence Lifetime-Transient Scanning (FLITS) 

DCS-120 Wideband, DCS-120 Multiphoton, and DCS-120 Macro FLIM systems 

2012 DCS-120 electronic alignment, NIR FLIM, FLITS integrated in DCS-120 and LSM 710 FLIM systems, 5th edition of bh TCSPC Handbook 

2 bh catalog 2012

Modular TCSPC systems 

1500 TCSPC Systems Worldwide 

SPC-630 TCSPC module 

Multi-dimensional photon distribution mode 

Photon stream (parameter tag) mode; buffer size 256 k photons 

Multi-detector / multi-wavelength operation 

FCS in combination with fluorescence lifetime 

Burst-integrated fluorescence lifetime detection 

Fast unlimited sequential recording 

Fast multiplexing of excitation sources 

Total recordable count rate 4 MHz, saturated count rate 8 MHz 

Time channel width 813 fs 

Each channel expandable for up to 8 detectors 

16-channel multi-wavelength detection with PML-SPEC assembly 

SPC-830 TCSPC module 


Photon stream (parameter tag) mode; buffer size 8 M photons 


Triggered MCS (phosphorescence) mode 

Synchronisation of channels for photon correlation 

Fast scanning: single- and multi-wavelength FLIM module for laser scanning 

microscopes 



Fast sequential recording 



Time channel width 813 fs 

Expandable with up to 8 detectors or 16-channel multispectral detection 

16-channel multi-wavelength detection with PML-SPEC assembly 

SPC-134 Four-Channel TCSPC package 

Four fully parallel TCSPC channels 







Unlimited fast sequential recording: TCSPC module for DOT systems 


Total recordable count rate 20 MHz 


SPC-154 Four-Channel TCSPC package 

Four fully parallel TCSPC channels 






Fast scanning: High-speed FLIM module for laser scanning microscopes 







SPC-130 low cost TCSPC Modules 

Standard fluorescence lifetime, TCSPC FLIM, Diffuse optical tomography, Singlemolecule 

spectroscopy 

Rugged design 

Overvoltage-protected inputs 

Photon distribution, time-tag, sequential-recording modes 

Low price, especially at large order volumes 

SPC-130-EM TCSPC Modules 

Improved SPC-130 with fast bus interface and larger memory 

Standard fluorescence lifetime, TCSPC FLIM, Diffuse optical tomography, Singlemolecule 

spectroscopy 

Rugged design 

Photon distribution, time-tag, sequential-recording modes 

Also available as SPC-134 EM 4-module package 

SPC-150: High-end performance at reasonable cost 





High-speed FLIM module for laser scanning microscopes 





Recordable count rate 5 MHz, saturated count rate 10 MHz 

Expandable with up to 8 detectors or 16-channel multispectral detection 

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Simple-Tau Compact TCSPC and TCSPC FLIM Systems 

Simple-Tau 830 compact TCSPC systems 

Recording by one SPC-830 TCSPC module 

Detector control via DCC-100 detector controller card 

Compact FLIM systems for laser scanning microscopy 

General time-resolved fluorescence spectroscopy 

Single-molecule spectroscopy 

Combined FCS and fluorescence lifetime systems 

Burst-integrated fluorescence lifetime experiments 

Compatible with bh multispectral FLIM detectors 


One SPC-150 TCSPC channel 

One DCC-100 detector controller 

Portable DOT systems 

High-Speed FLIM systems 

Compatible with bh multispectral FLIM detectors 

Part of bh DCS-120 confocal scanning FLIM systems 

Part of bh FLIM systems for Zeiss LSM 510 and LSM 710 family 

Part of bh NDD FLIM systems for Leica SP2 and SP5 MP 


Two parallel SPC-150 TCSPC channels 



High-Speed FLIM systems 

Part of bh DCS-120 confocal scanning FLIM systems 

Part of bh FLIM systems for Zeiss LSM 510 and LSM 710 family 


Four parallel SPC-150 TCSPC channels 


Ultra-High-Speed parallel multispectral FLIM systems 


Simple-Tau 130 EM compact TCSPC systems 

One SPC-130 or SPC-130 EM TCSPC channel 


Standard fluorescence lifetime applications 

Anti-bunching measurements 

Multi-Tau 8-Channel TCSPC systems 

Eight SPC-150 or SPC-130 TCSPC modules controlled from a standard Pentium PC 


Ultra-High-Speed FLIM systems 

Picosecond Photon Correlators 

DPC-230 16 Channel Picosecond Photon Correlator 

Recording of absolute photon times in 16 channels 

Fluorescence correlation down to ps times 

FCS combined with fluorescence decay 

3-channel reversed-start-stop TCSPC mode for PMT inputs 

15-channel reversed start-stop mode for LVTTL SPAD inputs 

15-channel multiscaler mode 

TCSPC FLIM Mode 

Multiscaler FLIM mode 

Autocorrelation / Cross correlation within 16 LVTTL or 4 CFD channels 

Simple-Tau DPC System 

Lap-top based compact system 

DPC-230 16 Channel Picosecond Photon Correlator 

Optional: 

DCC-100 detector controller card 

GVD-120 scan controller card 

4 bh catalog 2013

Multichannel Scalers 


MSA-300 Multichannel Scaler 

Recording of luminescence decay functions in the ns, and µs range 

Time-of-flight mass spectroscopy 

Lidar 

Time resolution 5 ns per time channel 

Maximum count rate 100 MHz 

Compact Simple-Tau MSA systems available 

PMS-400 Gated Photon Counter and Multichannel Scaler 

Dated detection of optical signals 

Luminescence decay in the µs and ms range 

Chemoluminescence 

Two parallel recording channels 

Gating down to 1 ns 

Multiscaler operation down to 300 ns per channel 

Simple-Tau MSA systems 

Lap-top based compact MSA-1000 or MSA-300 systems 

1,2, or 3 MSA-1000 modules 

1,2, 0r 3 MSA-300 modules 

DCC-100 detector controller 

Recording of luminescence decay functions in the ns, and µs range 

Time-of-flight mass spectroscopy 

Lidar 

Detectors and Detector Assemblies 

100 

mA/W 

-20 

-6 -1 

10 

-4 

-0 

-3 

1 

0.1 

PMC-100 

Cathode Radiant Sensitivity 

0.01 

200 300 400 500 600 700 800 900nm 

PMC-100 cooled PMT modules 

Cathode versions for UV to NIR region 

Internal PMT voltage generation 

IRF width 150 ps 

Ultra-stable IRF up to recorded count rates of 5 MHz 

Internal preamplifier 

Overload shutdown 

Power supply and control via DCC-100 detector controller 

Adapters for Zeiss LSM NDD ports, Zeiss LSM confocal ports, Leica SP2 and SP5 

RLD Ports, bh DCS-120 confocal FLIM system 

Part of the bh modular FLIM and modular DOT systems 

PMZ-100 PMT modules for FLIM Systems of Zeiss LSM 710 

Microscopes 

Adapter for Confocal (DC) port 

Adapter for non-descanned detection (NDD) port 

Internal PMT voltage generation 


Ultra-stable IRF up to recorded count rates of 5 MHz 

Internal preamplifier 

Overload shutdown 


PML-16 C 16-Channel TCSPC detectors 

16-channel PMT with routing electronics and internal HV power supply 

Simultaneous detection in 16 channels 

Connects directly to all bh SPC modules 


IRF width 150 to 200 ps 

Part of bh multispectral TCSPC FLIM detectors 

HPM-100 hybrid detector modules 

GaAsP versions: 40% detection efficiency throughout visible spectrum 

GaAs versions: 15% detection efficiency up to 850 nm 

No afterpulsing 

GaAsP versions: 130 ps IRF width 

Multialkali versions: 50 ps IRF width 

Internal high-voltage generator, controlled via bh DCC-100 

Active area 3 mm diameter 

C-Mount adapter 

Adapter to bh DCS-120 confocal scanning FLIM system 

Adapter to NDD port of Zeiss LSM 510 and LSM 710 NLO microscopes 

Adapter to RLD port of Leica SP2 MP and SP5 MP microscopes 

bh catalog 2013 5


Single-photon avalanche photodiode modules (SPADs) 

id Quantique id-100-20 and -50 SPADs 

Direct-coupled and fibre-coupled versions 

Ultra-high efficiency in visible region 


Active areas of 20µm, 50µm 

Stable IRF over count rate 

Dark count rates

Detector Electronics 


DCC-100 detector controller 

Power supply for two detectors 

Power supply of thermoelectric coolers 

Gain control and overload shutdown 

Control of shutters or other actuators 

Control of bh detector / shutter assemblies 

Power control of bh BHLP and BDL lasers 

Part of the bh modular FLIM, modular DOT, and Simple-Tau systems 

HVM-3000 High-Voltage module for PMTs and MCP-PMTs 

Small size, attaches directly to PMT or MCP 

SHV connector, connects directly to Hamamatsu R3809U MCP PMTs 

+12 V power supply via bh DCC-100 detector controller 

Output voltage -400 to -3000 V, controlled via DCC-100 detector controller 

HRT-41 four-channel router 

Connects up to four PMC-100, H7422 or R3809U detectors to one TCSPC channel 

All detectors are detecting simultaneously 

HRT-81 eight-channel router 

Connects up to eight PMC-100, H7422 or R3809U detectors to one TCSPC channel 


HRT-82 eight-channel router 

Connects up to eight SPAD modules to one TCSPC channel 


HFAC-26 Preamplifiers 

For R3809U MCP PMT, H7422 modules, and other PMTs 

Power supply from SPC or DCC module 

Overload shutdown in conjunction with DCC-100 controller 

Overload thresholds 0.1µA, 0,3µA, 1µA, 2µA, 3µA, 10µA 

HFAH-26 Preamplifiers 

Low-noise 

For R3809U MCP PMT, H7422 modules, and other PMTs 


Overload shutdown in conjunction with DCC-100 controller 

PHD-400 PIN Photodiode modules 

For synchronisation of TCSPC experiments with lasers 


Detector area 0.25 mm 2 

Current indicator for easy alignment 

Experiment Control 

DDG-200 Digital Delay Generator 

Multiplexing of lasers and routing into TCSPC Modules 

Multiplexing rate up to 100 kHz 

Non-overlapping multiplexing 

On-times programmable individually 

Part of the bh modular DOT systems 

Part of bh FLIM / PLIM system for Zeiss LSM 710 NLO 

Integrated in bh SPCM TCSPC instrument control software 

GVD-120 Scan Controller Card 

Generation of scan signals for galvanometer mirrors and piezo stages 

Controls also two bh BDL-SMC ps diode lasers 

Dual-axis control 

Frame scan, line scan, point operation 

Cycloid flyback 

Extremely high scan rates 

Laser beam blanking 

Fully digital signal generation 

Independent of software response times 

Pixel times down to 0.5 µs 

Pixel numbers up to 2048x2048 

Scan area definition interacts with images of SPCM software 

Part of bh DCS-120 confocal scanning systems 

Fully Integrated into SPCM software of bh TCSPC modules 

bh catalog 2013 7


Picosecond Diode Lasers 

New! BDS-SM series picosecond diode lasers 

Small-size OEM Module 

Dimensions 40 mm x 40 mm x 110 mm 

405 nm, 440 nm, 473 nm, 488 nm, 510 nm, 640 nm, 685nm, 785nm 

Free-beam or single-mode fibre output 

Pulse width down to 52 ps 

Pulse repetition rate 50 MHz 

CW equivalent power 0.25 to 1.2 mW 

Fast on / off / multiplexing capability 

All electronics integrated 

No external driver unit 

Simple +12 V power supply 

BDL-SMC Series Picosecond / CW diode lasers 

375 nm, 405 nm, 440 nm, 473 nm, 488 nm, 510 nm, 640 nm, 685nm, 785nm 

Beam corrector removes astigmatism and makes beam circular 

Interfaces with commonly used fibre couplers 

Repetition rate 20, 40, 80 MHz 

High-Power CW mode 

Pulse width typically 40 to 80 ps 

Fast on/off/multiplexing capability 

Fast electronic power control 

405 nm: 1 mW from single-mode fibre 

440, 473, 488, 640 nm: 0.4 mW from single-mode fibre 

Part of the bh modular FLIM systems for laser scanning microscopes 

The BDL-SMC lasers were designed in cooperation with Lasos GmbH, Jena 

Single-Mode Fibre Coupling 

Point-Source fibre coupler and fibre 

up to 70% coupling efficiency 

Fibres with FC output or with collimated output 

Part of DCS-120 Confocal Scanning FLIM Systems 

Easy Power supply and Control 

Power supply from simple wall mounted +12V power supply or from DCS box 

All laser electronics integrated laser head 

Interfaces directly with GVD-100 scan controller 

BDL-405-SMCP, BDL-440-SMCP, BDL-473-SMCP, BDL-488- 

SMCP picosecond diode lasers with fibre pigtails 

Single-mode fibre pigtail 

Single-mode fibre permanently attached to laser 

No fibre alignment required 

405 nm, 440 nm, 473 nm, 488 nm, 640 nm 

Designed in cooperation with Lasos GmbH, Jena 

BHL-600 picosecond diode lasers 

Wavelengths from 635 nm to 1300 nm 

Repetition rate 50 MHz 

Short pulse width typ. 40 to 50 ps 

Power typ. 0.3 mW 

All driver electronics integrated 

Simple wall mounted +12V power supply 

BHLP-700 picosecond diode lasers 

Wavelengths from 635 nm to 875 nm 

Repetition rate 50 MHz 

High power: Up to 10 mW at 785 nm, 5mW at 685 nm 

Pulse width typ. 80 ps at power

Fluorescence Lifetime Microscopy 


DCS-120 Confocal Scanning FLIM Systems 

Complete Confocal Laser Scanning FLIM microscopes 

FLIM upgrade for existing conventional microscopes 

Scanning by fast galvano mirrors 

Two fully confocal detection channels 

One or two input channels for bh BDL-SMC picosecond diode lasers 

Laser wavelengths 405 nm, 440 nm, 473, 488 nm, 640 nm 

High excitation power by beam-profile-corrected laser couplers 

One or two detection channels, parallel acquisition 

Channel separation by dichroic or polarising beamsplitters 

Individually selectable pinholes 

Individually selectable filters 

High efficiency by direct-coupled detectors 

GaAsP hybrid detectors 

16-channel multi-wavelength detector module 

Z-stack FLIM acquisition with Zeiss Axio Observer Z1 

New! Simultaneous fluorescence and phosphorescence lifetime imaging (PLIM) 

New! Fluorescence lifetime-transient scanning (FLITS) 

New! Multiphoton (MP) version 

New! Wideband (WB) version: Lasers freely swappable, compatible with tuneable 

lasers 

New! Electronic alignment 

New! DCS-120 Macro System 

FLIM of macroscopic objects 

Scan field up to 15 mm diameter 

Scanning by fast galvano mirrors 

Two fully confocal detection channels 

Laser wavelengths 405 nm, 440 nm, 473, 488 nm, 640 nm 

Wideband version for tuneable excitation 

GaAsP hybrid detectors 

FLIM Systems for Zeiss LSM 710 Family Microscopes 

LSM 710 NLO, LSM 780 NLO, LSM 7MP Multiphoton Microscopes 

LSM 710, LSM 780 Confocal Microscopes 

LSM 710, LSM 780 In Tune systems 

Multiphoton NDD FLIM 

Multiphoton NDD multispectral FLIM 

Confocal FLIM 

Confocal multispectral FLIM 

Confocal FCS, multiphoton FCS 

Fast preview mode 

Time-series FLIM 

Confocal Intune tuneable excitation FLIM and FCS 

Z Stack FLIM 

Unprecedented efficiency by GaAsP hybrid detectors 

Acquisition by 1, 2, 3 or 4 parallel TCSPC FLIM channels 

New! Simultaneous fluorescence and phosphorescence lifetime imaging (PLIM) 

New! Fluorescence lifetime-transient scanning (FLITS) 

New! Integrated version - operated from LSM 710 keyboard 

FLIM Systems for Zeiss LSM 510 NLO Multiphoton 

Microscopes 

Non-descanned detection 

Detectors connected to Zeiss NDD switch box 

All options of NDD light path available 

Single-wavelength NDD FLIM 

Dual-wavelength NDD FLIM 

Multi-spectral NDD FLIM 

HPM-100-40 hybrid detectors 

ROI and Zoom functions of LSM 510 available 

Works at any scan rate of LSM 510 

Ultra-high time resolution: 30 ps IRF with MCP detector 

Fast acquisition, fast preview mode 

Full overload protection of detectors 

One or two parallel SPC-830 or SPC-150 TCSPC channels 

PC based systems or Simple-Tau 

Controlled by Zeiss LSM 510 in conjunction with bh SPCM software 

FLIM Systems for Zeiss LSM 510 Confocal Microscopes 

Excitation by bh BDL-405 SMC picosecond diode laser 

Confocal detection 

Single wavelength detection 

Multi-spectral detection 

ROI and Zoom functions of LSM 510 available 

Works at any scan rate of LSM 510 

Fast Acquisition, fast preview mode 

Overload protection of detectors 

PC based systems or Simple-Tau 

Controlled by Zeiss LSM 510 in conjunction with bh SPCM software 

bh catalog 2013 9


FLIM Systems for Leica SP2, SP5, SP8 Microscopes 

bh TCSPC technique fully integrated into Leica systems 

Confocal detection 

Direct (RLD) detection for multiphoton microscopes 

Single wavelength detection 

Multi-spectral detection 

FCS capability 

ROI and Zoom functions of SP2 and SP5 available 

Works at any scan rate of SP2 and SP5 

Wavelength scan function of SP2 applicable 

Fast Acquisition, fast preview mode 


Controlled by Leica SP2 or SP5 in conjunction with bh SPCM software 

Non-Descanned FLIM Systems for Leica SP2 MP, 

SP5 MP, SP8 MP Microscopes 

Non-descanned detection via Leica RLD port 

1 detector coupled directly to RLD port 

2 detectors via external beamsplitter 

Simple-Tau 150 or 152 TCSPC systems 

Acquisition in 1 or 2 parallel TCSPC FLIM channels 

bh PMC-100-1 cooled PMT modules 

New! HPM-100-40 hybrid detectors 

bh MW FLIM multi-spectral FLIM detector 

ROI and Zoom functions of SP2 and SP5 available 

Works at any scan rate of SP2 and SP5 

Wavelength scan function of SP2 applicable 

Fast acquisition, fast preview mode 

FLIM Systems for Olympus FV1000 and FV300 Confocals 

Excitation by bh BDL-405 SMC or BDL-473 SMC picosecond diode laser 

High efficiency by direct coupling of detectors 

Single-wavelength detection: PMT, MCP-PMT, or Hybrid PMT 

Multi-wavelength detection: bh PML-SPEC detector 


ROI and Zoom functions of FV1000 or FV300 available 

Works at any scan rate 

FCS capability 

Non-descanned FLIM Systems for Olympus FV1000 and 

FV300 Multiphoton Microscopes 

Multiphoton FV1000 and FV300 systems with inverted microscopes 

High efficiency by non-descanned FLIM detection 

Deep-tissue imaging capability 

Single-wavelength detection: PMT, MCP-PMT, or Hybrid PMT 

Multi-spectral FLIM by bh MW FLIM detector 


ROI and Zoom functions of FV1000 or FV300 available 

Works at any scan rate 

10 bh catalog 2013


Time-Domain Diffuse Optical Tomography 

Modular DOT systems 

Several BHLP-700 lasers of different wavelengths multiplexed 

Time-of -flight distributions in four wavelength channels and four spatial channels 

Four parallel detectors 

Four parallel TCSPC channels 

DDG-200 digital delay generator 

SPC-154 or SPC-134 TCSPC packages 

Detector control via DCC-100 cards 

Saturated count rates up to 40 MHz 

Multiplexing periods of lasers individually programmable 

Unlimited sequential recording by continuous flow mode 

Acquisition rate up to 20 time-of-flight distributions per second 

Connecting Electronics 

Delay Switch Box 

32 step passive delay line 

Delay from 0 to 31.5 ns in 0.5 ns steps 

Transmits any signal waveform 

Signal Bandwidth 1 GHz 

No noise, no jitter 

No external power supply needed 

Settings persist when box is disconnected from USB 

Easy adjustment of TCSPC SYNC path length 

Control integrated in bh SPCM TCSPC operating software 

BOB-4 Signal Distribution Box 

Distributes scan clock signals to four SPC modules 

Combines routing signals and other control signals into 15-pin control inputs of four SPC-130, 

-140, and -150 modules 

Auxiliary +12V input for power supply of amplifiers, routers, detectors 

BOB-1 Signal Distribution Box 

Combines routing signals and other control signals into 15-pin control inputs of one SPC-130, 

-140, and -150 module 

Auxiliary +12V input for power supply of amplifiers, routers, detectors 

DCS Connection Box 

Power supply and control of two bh BDL-SMC ps diode lasers 

Connects scan clocks and laser multiplexing signals of GVD-120 scan controller into two 

SPC-150 or SPC-830 modules and two BDL-SMC diode lasers 

Connects routing signals of two bh multi-spectral FLIM detectors into SPC modules 

Cables and Adapters 

A-PPI-D passive pulse inverters 

1:3, 1:4 power splitters and combiners 

Attenuators 

SMA cables 

SMA-to SMA adapters 

SMA-to BNC adapters 

Power supply cables for detectors 

Interface cables for bh FLIM systems for various laser scanning microscopes 

A reasonable set of cables and adapters is delivered with each bh SPC module or Simple-Tau 

system 

bh catalog 2013 11


TCSPC Literature from the Technology Leader 

Wolfgang Becker 

Advanced Time-Correlated Single Photon Counting 

Techniques 

400 pages, 349 figures, 557 references 

Springer 2005, ISBN 3-540-26047-1 

Please contact bh for your personal copy 

Wolfgang Becker 

The bh TCSPC Handbook 

5th Edition, 2012 


Available from www.becker-hickl.com 

Please contact bh for printed copies 

DCS-120 Confocal Scanning FLIM Systems 

Edition 2012 

204 pages, 165 references, 273 figures 


Modular FLIM Systems for Zeiss 

LSM 510 and LSM 710 Laser Scanning Microscopes 

5th edition, August 2012 



Leica MP-FLIM and D-FLIM 

Fluorescence Lifetime Microscopy Systems 



Modular FLIM Systems for Olympus Laser Scanning 

Microscope Fluo View FV1000 



BDL-SMC NUV and Blue Picosecond Diode Lasers 

User Handbook 



DPC-230 16 Channel Photon Correlator 

User Handbook 

April 2008 



PML-16-C 16 Channel Detector Head for 

Time-Correlated Single Photon Counting 

User Handbook 



12 bh catalog 2013

Instrument Software 


SPCM Data Acquisition Software for 

bh TCSPC modules 

One software for all bh TCSPC modules 

Full access to all functions of the boards 

Configurable for different instrument configurations 

Easy change between different configurations 

Parallel operation of up to four SPC or DPC modules 

Online display of images and curves 

Online calculation of FCS, PCH, and MCS traces 

Online fit of FCS curves 

Page stepping 

Accumulation and autosave functions 

Oscilloscope mode 

Single and repeat mode 

Sequential modes 

Imaging modes 

Time tag mode 

Time tag imaging mode 

Integrated scanner control 

Interaction of scanner control with display cursors 

2D and 3D display modes 

3D Curve mode, colour-intensity mode 

Software gating of image display 

Display of multi-wavelength FLIM images 

Saving, loading, and conversion of photon distributions, 

images, and time-tag data 

Loading of predefined setups 

2D and 3D data processing functions 

Automatic interaction with SPCImage FLIM data analysis 

Scanner control via GVD-120 card 

Z-Axis drive of Zeiss Axio Observer 

Emulation mode for working without SPC hardware 

Runs under Windows 2000, NT, XP, Vista 

The SPCM software is free. It can be downloaded from 

www.becker-hickl.com. 

Operating Software for DCC-100 Detector 

controller 

Safe operation of PMTs, PMT Modules, and MCPs 

Control of high voltage of PMT modules or high voltage power 

supplies 

Control of shutters 

Control of coolers 

DCC panel can be placed anywhere within SPCM or MSA 

software panels 

Runs under windows 2000, NT, XP, Vista 

The DCC software is free. It can be downloaded from 

www.becker-hickl.com. 

bh catalog 2013 13


Operating Software for bh Multichannel Scalers 

Setting of system parameters 

Configuration of measurement sequences 

Data display 

Data processing 

Load and save of measurement data and setup parameters 

Operation of up to four modules 

Emulation mode to work without hardware 

Runs under windows 2000, NT, XP, Vista 

The MSA and PMS software is free. It can be downloaded from www.beckerhickl.com 

at any time. 

Data Analysis Software 

SPCImage FLIM Data Analysis Software 

Deconvolution of single curves and FLIM data 

Single, double, and triple-exponential decay analysis 

Single and double-exponential FRET 

Display of lifetime images 

Display of FRET images 

Display of lifetimes, amplitudes, intensities or ratios of these 

parameters 

Histograms of lifetimes, amplitudes, intensities or ratios of these 

parameters 

Export of lifetime data 

Export of images 

Direct interaction with SPCM software 

Optispec FLIM Data Analysis Software 

Deconvolution of single curves and FLIM data 

Single, double, and triple-exponential decay analysis 

Single and double-exponential FRET 

Automatic processing of multi-curve data 

Automatic processing of multi-spectral FLIM data 

Automatic processing of sequential FLIM measurements 

Global fitting of selected parameters 

Display of lifetime images 

Display of FRET images 

Display of lifetimes, amplitudes, intensities or ratios of these 

parameters 

Histograms of lifetimes, amplitudes, intensities or ratios of these 

parameters 

Export of lifetime data 

Export of images 

Direct interaction with SPCM software 

DLL and Lab View Libraries for TCSPC and Multiscaler Modules 

DLL libraries are available for most bh data acquisition and experiment control modules. 

We recommend, however, to check whether the desired effects can be obtained by using the hardware 

control features of our modules. Please contact bh for details. 

14 bh catalog 2013


Design, Manufacturing and Quality Management 

A Strong Partnership 

bh and Dorazil form an alliance since 1993. Located in the same building, our 

companies closely cooperate at all stages through schematics design, hybrid 

circuit design, board layout, and computer-aided manufacturing and testing. 

Continuous quality management through the complete design and 

manufacturing flow results in high reliability and short delivery time both for 

prototypes and large order quantities. 

Continuous flow from design into manufacturing 

Hybrid-circuit, FPGA, and PCB design 

Electronics running off the mill 

High-density automatic SMD placement 

Automatic soldering 

ROHS lead-free 

Thin film and thick film hybrid circuits 

In-house manufacturing 

Automatic placement of components 

Automatic chip bonding 

ISO 9000 certified 

Quality management though whole manufacturing process 

Uncompromised quality at any number of pieces 

bh catalog 2013 15

Becker & Hickl GmbH 

Nahmitzer Damm 30, 12277 Berlin, Germany 

Tel. +49 / 30 / 787 56 32 Fax. +49 / 30 / 787 57 34 

email: info@becker-hickl.com 

www.becker-hickl.com

Becker & Hickl GmbH

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