Book of abstract 2008

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Cytotoxic and genotoxic influence of nano-sized titanium dioxide particles in human hepatoma cells HepG2 Jana Petković and Metka Filipič Dept. of Genetic Toxicology and Cancer Biology, National Institute of Biology, Večna pot 111, SI-1000 Ljubljana, Slovenia Titanium dioxide (TiO 2 ) is widely used in the production of cosmetics, paints, paper, plastics and welding rod-coating material because of its low toxicity. However, recent studies have shown that TiO 2 nanoparticles (
CD44 and CD155 expression on human glioma in vitro: a flow cytometric, immunocytochemical and TIRF microscopy study of invasion indicators M. Navarros Rego, N. Karatas, G. Whitaker, P. B. Nunn, P. Warren, G. J. Pilkington Cellular & Molecular Neuro-oncology Group, Institute of Biomedical & Biomolecular Sciences, School of Pharmacy & Biomedical Sciences, University of Portsmouth, White Swan Road Portsmouth PO1 2DT, UK The poliovirus receptor, CD155 (PVR) is expressed on neoplastic glia and has already been used in therapeutic targeting of glioma. Recently CD155 has been proposed as playing a key role in glioma motility and invasion. CD44 is a cell adhesion molecule, originally described as the lymphocyte homing receptor, which is has two isoforms with respective molecular weights of 80-90kDa and 150kDa. The lower molecular weight isoform mediates attachment to hyaluronic acid (HA), which is present in relatively high concentration within the brain. CD44 is over-expressed in CNS and mediates both mediates glioma cell adhesion and invasion. Moreover, CD155 resides proximal to CD44 on the cell membrane of monocytes. The interactive role of the two molecules, CD44 and CD155, therefore merits investigation in the context of brain tumour invasiveness. Our aims were, therefore, to evaluate the expression levels of, and spacial relationship between, CD44 and CD155 in cultured glioma at high and low passage. High and low passage in vitro cultures of glioma were immunocytochemically stained using CD44 and CD155 antibodies and imaged by epi-fluorescence. Quantitative analysis was obtained by flow cytometry and the special relationship between the two epitopes on the cell surface was elucidated by total internal reflected fluorescence (TIRF) microscopy. Immunocytochemistry showed both CD44 and CD155 to be expressed at high and low passage numbers of glioblastoma in vitro. Double staining revealed both antigens to be expressed on the cell membrane at close but distinct sites. Flow cytometry revealed a higher expression level of CD44 compared with CD155 on all cultures tested. Having shown that these two molecules are co-expressed and are closely apposed on the cell membranes of glioma cells and are both involved in migration and invasion will are now aiming to carry out live cell imaging and Transwell Boyden chamber assays. In these experiments the influence of monoclonal antibody blocking and siRNA knock-down for both molecules, singularly and together will be used to establish any synergy or additive effect of CD44 and CD155 on glioma invasion. Acknowledgements: M Navarros Rego was supported by a Leonardo da Vinci Scholarship. p37119
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Co-chairs of the conference: Janko
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Table of Contents Conference Progra
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12:40 - 12:55 Irina Kondakova: Matr
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18:15 - 18:40 Ib Jarle Christensen:
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Abstracts of Lectures
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inhibitor of metalloproteinases-1 i
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Tyrosine kinase inhibitors increase
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Cancer gene therapy by electrotrans
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effect of tamoxifen. From a clinica
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Cox-2 is a target gene of Rho GDP d
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Cancer preventive potential of xant
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Gene expression regulation by siRNA
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Aptamer-based capture molecular as
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The expression pattern of the uroki
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Tumour vascular disrupting agents:
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Matrix metalloproteinases, their ti
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Altered expression of cysteine cath
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l22 37 Adult stem cells: from bench
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l24 39 Specific laboratory animal h
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The pharmacogenetic basis for indiv
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Mechanisms in metastasis Ruth J. Mu
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The lymphatic ring assay: a new in
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The use of immuno-nanoparticles for
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after antiangiogenic strategies, su
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The role of integrative genomics/pr
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Effect of the tumour microenvironme
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Addressing the angiogenic switch by
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Genetic predisposition on breast ca
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Kallikrein-related peptidases: nove
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Vascular disrupting action of elect
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Cysteine cathepsins in tumors and t
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Cysteine cathepsins and stefins in
Page 67 and 68: l50 67 Time dependence of electric
Page 69 and 70: Melanoma genomics and molecular tar
Page 71 and 72: Response to VEGF receptor inhibitio
Page 73 and 74: l55 73 Chemotherapy-induced cell de
Page 75 and 76: Towards high throughput, high conte
Page 77: l59 77 Mechanisms of proteolytic tu
Page 80 and 81: List of Posters P1. Tina Batista Na
Page 82 and 83: P37. Geoffrey J. Pilkington: CD44 a
Page 84 and 85: Mistletoe extract triggers mitochon
Page 86 and 87: Glioma: combating the invasive cell
Page 88 and 89: Protease inhibitory and cytotoxic e
Page 90 and 91: Delivery of meta-tetra(hydroxypheny
Page 92 and 93: Platinum analogue trans-[PtCl 2 (3-
Page 94 and 95: Reduction of plasminogen activity i
Page 96 and 97: Cysteine cathepsins and their endog
Page 98 and 99: The influence of Mg ions on the eff
Page 100 and 101: Dimerization of endogenous MT1-MMP
Page 102 and 103: Chemical acylation improves membran
Page 104 and 105: Comparison of the results for the J
Page 106 and 107: Tumor blood flow modifying changes
Page 108 and 109: Sodium iodide method is suitable fo
Page 110 and 111: Gene transfer into solid tumors by
Page 112 and 113: Reversal of thiopurine cytotoxicity
Page 114 and 115: Quantification of viability in orga
Page 116 and 117: Electrochemotherapy of cutaneous me
Page 120 and 121: Antiproliferative and adhesion-stim
Page 122 and 123: Mushrooms as a source of antitumour
Page 124 and 125: Avoiding systemic toxicity of the T
Page 126 and 127: Advantage of nanoparticles to deliv
Page 128 and 129: The influence of hypoosmolar medium
Page 130 and 131: Xanthohumol may affect cancer progr
Page 132 and 133: List of participants Amberger Murph
Page 134 and 135: Genova Kalou Petia National Center
Page 136 and 137: Korolenko Tatiana Institute of Phys
Page 138 and 139: Opolon Paule Institut Gustave-Rouss
Page 140 and 141: Sedmak Bojan National Institute of
Page 142 and 143: Voulgari Angeliki National Hellenic
Page 144 and 145: E Edwards Dylan 20 Edwards Dylan R.
Page 146 and 147: Pavelić Krešimir 51 Pavlič Janez
Page 148 and 149: We believe in our future! Photo: Ma
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Page 154 and 155: TANTUM VERDE 154
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