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Mistletoe extract triggers mitochondrial pathway <strong>of</strong> apoptosis by<br />
influencing the expression <strong>of</strong> CK II, Mcl-1 and PRDX3 proteins<br />
Petra Slanc1, Jernej Kristl1, Aleš Berlec2, Polona Jamnik3, Nataša Obermajer1, Janko Kos1,<br />
Borut Štrukelj1 ,2<br />
1Dept. <strong>of</strong> Pharmaceutical Biology, Faculty <strong>of</strong> Pharmacy, University <strong>of</strong> Ljubljana, Aškerčeva 7, SI-1000<br />
Ljubljana, Slovenia; 2Dept. <strong>of</strong> Biotechnology, Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana,<br />
Slovenia; 3Chair <strong>of</strong> Biotechnology, Biotechnical Faculty, University <strong>of</strong> Ljubljana, Jamnikarjeva 101, SI-<br />
1000 Ljubljana, Slovenia<br />
European mistletoe (Viscum album) extracts have been used as anti-cancer agents in<br />
complementary medicine for centuries. Cytotoxic and immunomodulatory effects have<br />
been reported in in vivo and in vitro studies. Their precise mode <strong>of</strong> action however remains<br />
unknown. We studied the effects <strong>of</strong> commercially available extract on two leukemic cell<br />
lines ( Jurkat, Molt). Differential expression <strong>of</strong> proteins was monitored on transcriptional<br />
and translational level. After treatment with the extract we observed differential expression<br />
<strong>of</strong> proteins involved in pro-survival and apoptotic processes (CKII, Mcl-1) and several<br />
mitochondrial proteins including PRDX3. Our results indicate that mistletoe extract<br />
initiates mitochondrial pathway <strong>of</strong> apoptosis.<br />
p2<br />
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