Book of abstract 2008

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P37. Geoffrey J. Pilkington: CD44 and CD155 expression on human glioma in vitro: a flow cytometric, immunocytochemical and TIRF microscopy study of invasion indicators P38. Jure Pohleven: Antiproliferative and adhesion-stimulating effects on human cancer cell lines elicited by lectins from mushroom Clitocybe nebularis P39. Anja Pucer: Arsenite and the lysosomal cysteine cathepsins in cell death of U87 glioblastoma cells P40. Jerica Sabotič: Mushrooms as a source of antitumour substances P41. Karin Schara: Effect of heparin on microvesiculation of membranes. A possible mechanism of treatment of Trousseau syndromme P42. Vanja Smilović: Avoiding systemic toxicity of the TNF superfamily ligands: induction of cell death without ligands P43. Marko Snoj: Limb sparing treatment of bleeding melanoma recurrence by electrochemotherapy P44. K. Teskač: Advantage of nanoparticles to deliver resveratrol into the cells P45. L. Towhidi: Combined effect of pulsed electric and magnetic field on CHO cells dye uptake P46. Marko Ušaj: The influence of hypoosmolar medium and electric field on cell volume P47. Angeliki Voulgari: TAF12 is regulated by ras, to mediate the alteration of E- cadherin expression in epithelial-to-mesenchymal transition (EMT) (1) P48. Irena Zajc: Xanthohumol may affect cancer progression with its differential cytotoxicity and apoptosis induction in normal and cancer cell lines P49. Anže Županič: Optimization of electrode position and electric parameters in electrochemotherapy 82
High-voltage nanosecond electrical pulses affect plasma membrane of cultured cells Tina Batista Napotnik1, Matej Reberšek1, Danilo Vrtačnik2, Slavko Amon2, Damijan Miklavčič1 1Laboratory of Biocybernetics, Faculty of Electrical Engineering, Tržaška 25, 1000 Ljubljana, Slovenia; 2Laboratory of Microsensor Structures and Electronics, Faculty of Electrical Engineering, Tržaška 25, Ljubljana, Slovenia The exposure of cells to an electric field results in poration of the plasma membrane (i.e. electroporation). The electroporation is already in use in clinical treatments in electrochemotherapy and gene electrotransfection. Typical pulses that are used for the electroporation are in micro- to millisecond time range and with electrical field strength of 100 to a few 1000 V/cm. High-voltage nanosecond electrical pulses (with duration from 1 to 100 ns and the electric field strength up to a few 100 kV/cm) can be achieved by high-voltage generators, usually derived from a Blumlein pulse-forming network. These pulses are short enough and high enough to induce sufficiently high induced voltage across the intracellular membranes. The nanosecond pulses have greater effect on cell organelles than on the plasma membrane: they can induce pore formation in membranes of cell organelles. This can lead to elevating cell calcium, DNA damage and apoptosis. When pulse parameters (duration, voltage, and number of pulses) are augmented, also the effects on plasma membrane increase. We have designed a modified Blumlein generator for generating high-voltage nanosecond electrical pulses of various durations (10 to 300 ns) and pulse frequency in a pulse train (up to very high frequencies of a few 100 kHz). The electrodes made of gold are deposited onto a microscope cover glass and placed under a fluorescence microscope in order to observe immediate effects on cultured cells. The gap between the two electrodes is 100 μm so very high field strength p1 can be achieved (up to 100 kV/cm). The B16 F1 mouse melanoma cells were exposed to high-voltage nanosecond pulses (60 ns, 44 kV/cm) of various repetitition frequency (up to 100 Hz) and number of pulses (up to 60) in a pulse train. We observed cell uptake of propidium iodide (PI) after the pulsing. We confirmed that higher number of pulses and higher frequency of pulses in a pulse train cause rise of plasma membrane permeability, therefore cells become positive for PI staining. These results show that high-voltage nanosecond pulses in higher frequencies and number of pulses in a pulse train affect plasma membrane of cultured cells. 83
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Co-chairs of the conference: Janko
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Table of Contents Conference Progra
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12:40 - 12:55 Irina Kondakova: Matr
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18:15 - 18:40 Ib Jarle Christensen:
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Abstracts of Lectures
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inhibitor of metalloproteinases-1 i
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Tyrosine kinase inhibitors increase
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Cancer gene therapy by electrotrans
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effect of tamoxifen. From a clinica
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Cox-2 is a target gene of Rho GDP d
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Cancer preventive potential of xant
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Gene expression regulation by siRNA
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Aptamer-based capture molecular as
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The expression pattern of the uroki
Page 31 and 32: Tumour vascular disrupting agents:
Page 33 and 34: Matrix metalloproteinases, their ti
Page 35 and 36: Altered expression of cysteine cath
Page 37 and 38: l22 37 Adult stem cells: from bench
Page 39 and 40: l24 39 Specific laboratory animal h
Page 41 and 42: The pharmacogenetic basis for indiv
Page 43 and 44: Mechanisms in metastasis Ruth J. Mu
Page 45 and 46: The lymphatic ring assay: a new in
Page 47 and 48: The use of immuno-nanoparticles for
Page 49 and 50: after antiangiogenic strategies, su
Page 51 and 52: The role of integrative genomics/pr
Page 53 and 54: Effect of the tumour microenvironme
Page 55 and 56: Addressing the angiogenic switch by
Page 57 and 58: Genetic predisposition on breast ca
Page 59 and 60: Kallikrein-related peptidases: nove
Page 61 and 62: Vascular disrupting action of elect
Page 63 and 64: Cysteine cathepsins in tumors and t
Page 65 and 66: Cysteine cathepsins and stefins in
Page 67 and 68: l50 67 Time dependence of electric
Page 69 and 70: Melanoma genomics and molecular tar
Page 71 and 72: Response to VEGF receptor inhibitio
Page 73 and 74: l55 73 Chemotherapy-induced cell de
Page 75 and 76: Towards high throughput, high conte
Page 77: l59 77 Mechanisms of proteolytic tu
Page 80 and 81: List of Posters P1. Tina Batista Na
Page 84 and 85: Mistletoe extract triggers mitochon
Page 86 and 87: Glioma: combating the invasive cell
Page 88 and 89: Protease inhibitory and cytotoxic e
Page 90 and 91: Delivery of meta-tetra(hydroxypheny
Page 92 and 93: Platinum analogue trans-[PtCl 2 (3-
Page 94 and 95: Reduction of plasminogen activity i
Page 96 and 97: Cysteine cathepsins and their endog
Page 98 and 99: The influence of Mg ions on the eff
Page 100 and 101: Dimerization of endogenous MT1-MMP
Page 102 and 103: Chemical acylation improves membran
Page 104 and 105: Comparison of the results for the J
Page 106 and 107: Tumor blood flow modifying changes
Page 108 and 109: Sodium iodide method is suitable fo
Page 110 and 111: Gene transfer into solid tumors by
Page 112 and 113: Reversal of thiopurine cytotoxicity
Page 114 and 115: Quantification of viability in orga
Page 116 and 117: Electrochemotherapy of cutaneous me
Page 118 and 119: Cytotoxic and genotoxic influence o
Page 120 and 121: Antiproliferative and adhesion-stim
Page 122 and 123: Mushrooms as a source of antitumour
Page 124 and 125: Avoiding systemic toxicity of the T
Page 126 and 127: Advantage of nanoparticles to deliv
Page 128 and 129: The influence of hypoosmolar medium
Page 130 and 131: Xanthohumol may affect cancer progr
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List of participants Amberger Murph
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Genova Kalou Petia National Center
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Korolenko Tatiana Institute of Phys
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Opolon Paule Institut Gustave-Rouss
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Sedmak Bojan National Institute of
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Voulgari Angeliki National Hellenic
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E Edwards Dylan 20 Edwards Dylan R.
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Pavelić Krešimir 51 Pavlič Janez
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We believe in our future! Photo: Ma
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TANTUM VERDE 154
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