Cell Culture

1 Human Primary Cells 21
1.5 Epithelial Cells
Applications
Drug uptake studies
Toxicological research
Wound healing research
Oncology
Cell differentiation studies
Respiratory disease research
Features
Cytokeratin positive
Available from different tissues
Cryopreserved at second passage
Typically, epithelial tissue is made of
tightly packed cells with little intercellular
space and is arranged in flat sheets
forming effective barriers between the
underlying tissues and the external environment.
PromoCell offers epithelial cells from different
locations:
Airways: Nasal mucosa, trachea, bronchi,
and distal respiratory tract
Breast: Mammary gland
Kidney: Whole kidney and renal cortex
Placenta: Amniotic membrane
Fig. 1: HTEpC culture in phase contrast.
Note the cuboidal shape typical for airway
epithelial cells.
1 Human Primary
Cells
The epithelium is the interface between the
body and the external environment. It covers
all outside surfaces and inside lumina
e.g. the inner lining of the respiratory
tract. In addition, epithelial cells also make
up exocrine and endocrine glands. The
functions of epithelial cells are diverse and
include absorption, secretion, protection,
transcellular transport, and sensation.
Available Epithelial Cells:
HNEpC (see page 21)
HTEpC (see page 21)
HBEpC (see page 22)
HSAEpC (see page 22)
HMEpC (see page 23)
HREpC (see page 23)
HRCEpC (see page 24)
HPlEpC (see page 24)
Fig. 2: HTEpC culture stained for cytokeratin.
Nuclei counterstained with
DAPI.
Human Nasal Epithelial Cells
(HNEpC)
HNEpC are isolated from normal human
nasal mucosa and stain positive for cytokeratin.
The epithelium in the nasal cavity cleans,
humidifies, and warms inhaled air. In addition,
it produces mucus, which binds
particles that are subsequently transported
to the pharynx by cilia on the
epithelial cells. HNEpC are useful for in
vitro studies of these processes.
Recommended media & reagents
Airway Epithelial Cell Growth Medium
(see page 71)
DetachKit (see page 101)
Cryo-SFM (see page 85)
Related products
HNEpC Pellet (see page 98)
PromoFectin Transfection Reagent
(see PromoKine catalog)
https://www.promocell.com/shop
Product Size Catalog Number
Human Nasal Epithelial Cells (HNEpC)
500,000 cryopreserved cells
500,000 proliferating cells
C-12620
C-12621
HNEpC Pellet 1x10 6 cells in RNAlater ® C-14062
Human Tracheal Epithelial Cells
(HTEpC)
HTEpC are isolated from the surface
epithelium of human trachea and stain
positive for cytokeratin.
The respiratory epithelia are responsible
for the lubrication of the lungs,
the maintenance of humidity, and the
cleaning of the respiratory tract. They
are an important target for drugs, toxins,
and carcinogens. In addition, they
are involved in many diseases such as
cystic fibrosis. Consequently, HTEpC are
useful for investigating the function and
pathology of the respiratory system.
Recommended media & reagents
Airway Epithelial Cell Growth Medium
(see page 71)
DetachKit (see page 101)
Cryo-SFM (see page 85)
Related products
HTEpC Pellet (see page 98)
Human Tracheal Smooth Muscle Cells
(see page 37)
PromoFectin Transfection Reagent
(see PromoKine catalog)