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Cell Culture

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4 Media and Sera 85<br />

Low Serum<br />

4.1.14 Smooth Muscle <strong>Cell</strong> Medium<br />

Smooth Muscle <strong>Cell</strong> Growth<br />

Medium 2<br />

Recommended for<br />

Human Umbilical Artery Smooth<br />

Muscle <strong>Cell</strong>s (see page 37)<br />

Human Coronary Artery Smooth<br />

Muscle <strong>Cell</strong>s (see page 36)<br />

Human Pulmonary Artery Smooth<br />

Muscle <strong>Cell</strong>s (see page 36)<br />

Human Aortic Smooth Muscle <strong>Cell</strong>s<br />

(see page 35)<br />

Human Bronchial Smooth Muscle<br />

<strong>Cell</strong>s (see page 38)<br />

Human Tracheal Smooth Muscle <strong>Cell</strong>s<br />

(see page 37)<br />

Human Bladder Smooth Muscle <strong>Cell</strong>s<br />

(see page 38)<br />

Human Prostate Smooth Muscle <strong>Cell</strong>s<br />

(see page 39)<br />

Human Uterine Smooth Muscle <strong>Cell</strong>s<br />

(see page 39)<br />

The Promo<strong>Cell</strong> Smooth Muscle <strong>Cell</strong><br />

Growth Medium 2 is a low-serum (5%<br />

V/V) medium developed to establish and<br />

maintain smooth muscle cell cultures.<br />

The medium is optimized for primary<br />

human cells but can also be used for<br />

bovine, porcine, murine, dog, and rat<br />

smooth muscle cells.<br />

Related products<br />

Fetal Calf Serum (see page 92)<br />

Final supplement concentrations<br />

(after addition to the medium)<br />

Fetal Calf Serum<br />

Epidermal Growth Factor<br />

(recombinant human)<br />

Basic Fibroblast Growth Factor<br />

(recombinant human)<br />

Insulin (recombinant human)<br />

0.05 ml / ml<br />

0.5 ng / ml<br />

2 ng / ml<br />

5 μg / ml<br />

https://www.promocell.com/shop<br />

Product Size Catalog Number<br />

Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />

500 ml C-22062<br />

(Ready-to-use)<br />

Smooth Muscle <strong>Cell</strong> Growth Medium 2 Kit 500 ml C-22162<br />

Smooth Muscle <strong>Cell</strong> Basal Medium 2 500 ml C-22262<br />

Smooth Muscle <strong>Cell</strong> Basal Medium 2,<br />

500 ml C-22267<br />

phenol red-free<br />

Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />

SupplementMix<br />

for 500 ml C-39267<br />

4 Media and Sera<br />

Smooth Muscle <strong>Cell</strong> Growth Medium 2<br />

SupplementPack<br />

for 500 ml C-39262<br />

4.1.15 Freezing Medium<br />

Serum-Free<br />

Recommended for<br />

Human Primary <strong>Cell</strong>s<br />

Animal Primary <strong>Cell</strong>s<br />

<strong>Cell</strong> Lines<br />

The Promo<strong>Cell</strong> Cryo-SFM is an animal<br />

component-free, chemically defined medium<br />

with DMSO, methylcellulose, and<br />

other cryoprotective components developed<br />

for the cryopreservation of human<br />

and animal primary cells. It ensures high<br />

cell viability, excellent growth rates, and<br />

good attachment of the cells after thawing.<br />

The medium is the first choice for all<br />

types of primary cells and cell lines.<br />

https://www.promocell.com/shop<br />

Product Size Catalog Number<br />

Cryo-SFM<br />

30 ml<br />

125 ml<br />

C-29910<br />

C-29912

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