Cell Culture

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52 2 Human Stem and Blood Cells 2.5 Monocytes Applications gradient-based method completely Recommended media & reagents devoid of using any immunomagnetic Mononuclear Cell Medium (see page 89) Dendritic Cell Research beads. The advantage of these com- DC Generation Medium (see page 88) (Auto-)Immunity pletely native Monocytes is that poten- Infection biology tial effects of bound magnetic particles Related products Features (which can be ingested by this phagocytic cell type) are excluded. hMoCD14 + -PB single donor Pellet (see page 98) hMoCD14 + -PB single donor Isolated from fresh peripheral blood Routinely analyzed by flow cytometry CD14 + -selected as well as “untouched“ Human CD14 + Monocytes (hMoCD14 + -PB) pre-screened Pellet (see page 98) hMo-PB single donor Pellet (see page 98) 2 Human Stem and Blood Cells Monocytes available Monocytes (Mo) are immature phagocytic cells circulating in the blood. Acting PromoCell Human CD14 + Monocytes are isolated from fresh peripheral blood. After isolation of the ultra-pure mono- hMo-PB single donor pre-screened Pellet (see page 98) PromoFectin-Macrophage Transfection Reagent as antigen-presenting immune cells, they nuclear cell fraction by proprietary meth- (see PromoKine catalog) can phagocytize and degrade microbes ods, the CD14 + Monocytes are isolated G-CSF (see page 106) and particulate matter. Monocytes can by positive selection with > 95% purity. GM-CSF (see page 106) differentiate into several different cell IL-4 CC (see page 106) types. The most common applications In addition, hMoCD14 + -PB are available TNF-alpha (see page 106) for Monocytes are the in vitro differen- which are pre-screened for their differ- M-CSF (see page 106) tiation into monocyte-derived dendritic entiation capacity into dendritic cells. sRANKL (see page 106) cells (moDC), macrophages, and osteo- CD14 antibody clasts as well as their use as phagocytic immune cells in immunology and infec- Human Monocytes (hMo-PB) (see www.promokine.info) tion biology. PromoCell Human “untouched” Mono- CD14 belongs to the family of LPS re- cytes are isolated from fresh peripheral ceptor antigens and is strongly expressed blood. After isolation of the ultra-pure on the majority of Monocytes. Thus, mononuclear cell fraction by proprietary binding of immunomagnetic particles methods, the Monocyte fraction is fur- to this antigen is employed for purifica- ther purified to > 70% by an advanced tion of PromoCell CD14 + -Monocytes multi-gradient technique. (hMoCD14 + -PB) from Mononuclear Cells (MNC). In contrast, the “untouched” Monocytes (hMo-PB) provided by PromoCell In addition, hMo-PB are available which are pre-screened for their differentiation capacity into dendritic cells. Fig. 8: Cluster formation during differentiation of Monocytes into moDC. are enriched from MNC by a density For more information please see www.promocell.com/knowledge-base Fig. 9: Mature moDC cultured in PromoCell DC Generation Medium showing typical dendrite-like structures.
2 Human Stem and Blood Cells 53 Count Fig. 10a: Flow cytometric analysis of day 8 mature moDCs generated in the PromoCell DC Generation Medium. More than 98% of the cells are negative for CD14. 12,5 0 0 CD14 - CD14 + 10 1 10 2 10 3 10 4 CD14 Cell Lab Quanta SC TM , Beckman Coulter, Inc. 2 Human Stem and Blood Cells Count Fig. 10b: Flow cytometric analysis of day 8 mature moDCs generated in the PromoCell DC Generation Medium. The DC maturation marker CD83 is strongly expressed by > 98% of the cells. 12,5 0 0 CD83 - CD83 + 10 1 10 2 10 3 10 4 CD83 Cell Lab Quanta SC TM , Beckman Coulter, Inc. https://www.promocell.com/shop Product Size Catalog Number Human CD14 + Monocytes from Peripheral Blood (hMoCD14 + -PB), single donor 10 x 10 6 new cryopreserved cells C-12909 hMoCD14 + -PB single donor Pellet 1x10 6 cells in RNAlater ® new C-14110 Human CD14 + Monocytes from Peripheral Blood (hMoCD14 + -PB), single donor, pre-screened hMoCD14 + -PB single donor pre-screened Pellet 10 x 10 6 new cryopreserved cells C-12912 1x10 6 cells in RNAlater ® new C-14112 Human Monocytes from Peripheral Blood 10 x 10 6 cryopreserved cells new C-12910 (hMo-PB), single donor hMo-PB single donor Pellet 1x10 6 cells in RNAlater ® new C-14111 Human Monocytes from Peripheral Blood 10 x 10 6 cryopreserved cells new C-12913 (hMo-PB), single donor, pre-screened hMo-PB single donor pre-screened Pellet 1x10 6 cells in RNAlater ® new C-14113
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Cell Culture 2012
Page 3 and 4: Overview 1 Overview 1 Human Primary
Page 5 and 6: Contents 3 5 6 new Cell Pellets 5.1
Page 7: About PromoCell 5 The PromoCell Cus
Page 10 and 11: 8 Content Chapter 1 1 Human Primary
Page 12 and 13: 10 1 Human Primary Cells 1.2 Chondr
Page 14 and 15: 12 1 Human Primary Cells 1 Human Pr
Page 16 and 17: 14 1 Human Primary Cells Human Pulm
Page 20 and 21: 18 1 Human Primary Cells Human Blad
Page 22 and 23: 20 1 Human Primary Cells Human Brai
Page 24 and 25: 22 1 Human Primary Cells https://ww
Page 26 and 27: 24 1 Human Primary Cells https://ww
Page 28 and 29: 26 1 Human Primary Cells Human Pulm
Page 30 and 31: 28 1 Human Primary Cells 1.7 Follic
Page 32 and 33: 30 1 Human Primary Cells 1.9 Kerati
Page 34 and 35: 32 1 Human Primary Cells 1.11 Osteo
Page 36 and 37: 34 1 Human Primary Cells 1.13 Skele
Page 38 and 39: 36 1 Human Primary Cells Human Coro
Page 40 and 41: 38 1 Human Primary Cells Human Bron
Page 42 and 43: 40 1 Human Primary Cells Overview H
Page 46 and 47: 44 1 Human Primary Cells Product Pa
Page 48 and 49: 46 Content Chapter 2 2 Human Stem a
Page 50 and 51: 48 2 Human Stem and Blood Cells 2 H
Page 52 and 53: 50 2 Human Stem and Blood Cells 2.3
Page 56 and 57: 54 2 Human Stem and Blood Cells 2.6
Page 58 and 59: 56 2 Human Stem and Blood Cells Ove
Page 60 and 61: 58 Content Chapter 3 3 Cell Model S
Page 62 and 63: 60 3 Cell Model Systems Cumulative
Page 64 and 65: 62 3 Cell Model Systems Transmigrat
Page 67 and 68: Media and Sera 4 High-quality media
Page 69 and 70: 4 Media and Sera 67 4.1 Media for P
Page 71 and 72: 4 Media and Sera 69 Low Serum 4.1.3
Page 73 and 74: 4 Media and Sera 71 Serum-Free 4.1.
Page 75 and 76: 4 Media and Sera 73 Serum-Free Mamm
Page 77 and 78: 4 Media and Sera 75 Low Serum Place
Page 79 and 80: 4 Media and Sera 77 https://www.pro
Page 89 and 90: 4 Media and Sera 87 4.2.2 Pericyte
Page 91 and 92: 4 Media and Sera 89 List of supplem
Page 93 and 94: 4 Media and Sera 91 • McCoy´s 5A
Page 95: 4 Media and Sera 93 MSC-Qualified F
Page 98 and 99: 96 Content Chapter 5 5 Cell Pellets
Page 100 and 101: 98 5 Cell Pellets https://www.promo
Page 102 and 103: 100 Content Chapter 6 6 Reagents an
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102 6 Reagents and Supplements Cell
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104 6 Reagents and Supplements 6.3
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106 6 Reagents and Supplements 6.5
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108 Content Chapter 7 7 Microbial D
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110 7 Microbial Detection and Elimi
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112 7 Microbial Detection and Elimi
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PromoKine 8 Cell Biology Products P
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8 Cell Biology 117 8.1 Cell Analysi
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8 Cell Biology 119 8.7 Cytokines an
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Alphabetical Index 121 Description
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Alphabetical Index 123 Description
Page 127 and 128:
Numerical Index 125 Cat. Number Pro
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Page 133 and 134:
International Distributors 131 Inte
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International Distributors 133 Pola
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Terms and Conditions 135 properly p
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Fax Order Form Shipping Address Inv
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