Cell Culture

More documents

Recommendations

Info

92 4 Media and Sera 4.4 Insect Cell Culture Media The medium requirements of insect cells are significantly different from vertebrate cell culture. For instance, the optimal pH range for most insect cells is more acidic (pH 6.2 to 6.5). Also, the amino acid concentration needs to be higher than in vertebrate cell media. Mitsuhashi/Maramorosh Insect Medium Recommended for Mitsuhashi and Maramorosh Insect Medium is well-suited for Mosquito cell culture and must be supplemented with FCS prior to use. Mosquito Cells https://www.promocell.com/shop Product Size Catalog Number Mitsuhashi/Maramorosh Insect Medium 500 ml C-77110 4 Media and Sera Schneider’s Drosophila Medium Recommended for Dipteran Cells Schneider’s Drosophila Medium was originally developed for the culture of Drosophila cells but can also be used for the culture of other dipteran cell lines. https://www.promocell.com/shop Product Size Catalog Number Schneider’s Drosophila Medium, w/o L-Glutamine 500 ml C-77210 4.5 Sera Fetal Calf Serum Recommended for Primary Human Cells (all types) The PromoCell Fetal Calf Sera are high quality, pretested fetal calf serum batches. They are extensively tested for their ability to support in vitro expansion of human primary cells. In addition, histochemical staining is performed to ensure that the cultured primary cells maintain their cell type specific marker expression. https://www.promocell.com/shop Product Size Catalog Number Fetal Calf Serum (EU Quality) primary human cell culture tested 100 ml 500 ml C-37355 C-37350
4 Media and Sera 93 MSC-Qualified Fetal Calf Serum Recommended for Mesenchymal Stem Cells (all types) The PromoCell MSC-Qualified Fetal Calf Sera are high quality, pre-selected fetal calf serum batches. They are extensively tested for their ability to support in vitro expansion of human Mesenchymal Stem Cells. In addition, histochemical staining is performed to ensure that the cultured stem cells maintain the ability to differentiate into diverse lineages. Fig. 1: Expansion and differentiation of Mesenchymal Stem Cells (MSC) using media with the PromoCell MSC-Qualified Fetal Calf Serum. Left: Undifferentiated hMSC-BM cultured for 4 passages (phase contrast). Right: The same cells after 14 days of adipogenic induction (Oil Red O staining of lipid droplets with Hematoxylin counterstain). https://www.promocell.com/shop Product Size Catalog Number MSC-Qualified Fetal Calf Serum 100 ml 500 ml C-37386 C-37385 ESC-Qualified Fetal Calf Serum The PromoCell ESC-Qualified Fetal Calf factor. The maintenance of pluripotency 4 Media and Sera Sera are high quality, pre-selected fetal is assayed by immunostaining for basal Recommended for calf serum batches. They are exten- pluripotency markers. In addition, this sively tested for their ability to support serum is also recommended for the cul- Pluripotent Embryonic Stem Cells undifferentiated, feeder-free culture of ture of hematopoietic and endothelial Hematopoietic Progenitor Cells pluripotent murine embryonic stem cells progenitor cells. Endothelial Progenitor Cells in the presence of leukemia inhibitory Fig. 2: Growth morphology of murine embryonic stem cells (mESC). Left: mESC in standard Fetal Calf Serum. Note the large, flat, differentiated cells. Right: mESC after 5 passages in PromoCell ESC-Qualified Fetal Calf Serum. The cells are small and have a compact colony morphology typical for mESC. https://www.promocell.com/shop Product Size Catalog Number ESC-Qualified Fetal Calf Serum 100 ml 500 ml C-37388 C-37387
Page 1 and 2:
Cell Culture 2012
Page 3 and 4:
Overview 1 Overview 1 Human Primary
Page 5 and 6:
Contents 3 5 6 new Cell Pellets 5.1
Page 7:
About PromoCell 5 The PromoCell Cus
Page 10 and 11:
8 Content Chapter 1 1 Human Primary
Page 12 and 13:
10 1 Human Primary Cells 1.2 Chondr
Page 14 and 15:
12 1 Human Primary Cells 1 Human Pr
Page 16 and 17:
14 1 Human Primary Cells Human Pulm
Page 18 and 19:
16 1 Human Primary Cells 1 Human Pr
Page 20 and 21:
18 1 Human Primary Cells Human Blad
Page 22 and 23:
20 1 Human Primary Cells Human Brai
Page 24 and 25:
22 1 Human Primary Cells https://ww
Page 26 and 27:
24 1 Human Primary Cells https://ww
Page 28 and 29:
26 1 Human Primary Cells Human Pulm
Page 30 and 31:
28 1 Human Primary Cells 1.7 Follic
Page 32 and 33:
30 1 Human Primary Cells 1.9 Kerati
Page 34 and 35:
32 1 Human Primary Cells 1.11 Osteo
Page 36 and 37:
34 1 Human Primary Cells 1.13 Skele
Page 38 and 39:
36 1 Human Primary Cells Human Coro
Page 40 and 41:
38 1 Human Primary Cells Human Bron
Page 42 and 43:
40 1 Human Primary Cells Overview H
Page 44 and 45: 42 1 Human Primary Cells 1 Human Pr
Page 46 and 47: 44 1 Human Primary Cells Product Pa
Page 48 and 49: 46 Content Chapter 2 2 Human Stem a
Page 50 and 51: 48 2 Human Stem and Blood Cells 2 H
Page 52 and 53: 50 2 Human Stem and Blood Cells 2.3
Page 58 and 59: 56 2 Human Stem and Blood Cells Ove
Page 60 and 61: 58 Content Chapter 3 3 Cell Model S
Page 62 and 63: 60 3 Cell Model Systems Cumulative
Page 64 and 65: 62 3 Cell Model Systems Transmigrat
Page 67 and 68: Media and Sera 4 High-quality media
Page 69 and 70: 4 Media and Sera 67 4.1 Media for P
Page 71 and 72: 4 Media and Sera 69 Low Serum 4.1.3
Page 73 and 74: 4 Media and Sera 71 Serum-Free 4.1.
Page 75 and 76: 4 Media and Sera 73 Serum-Free Mamm
Page 77 and 78: 4 Media and Sera 75 Low Serum Place
Page 79 and 80: 4 Media and Sera 77 https://www.pro
Page 89 and 90: 4 Media and Sera 87 4.2.2 Pericyte
Page 91 and 92: 4 Media and Sera 89 List of supplem
Page 93: 4 Media and Sera 91 • McCoy´s 5A
Page 98 and 99: 96 Content Chapter 5 5 Cell Pellets
Page 100 and 101: 98 5 Cell Pellets https://www.promo
Page 102 and 103: 100 Content Chapter 6 6 Reagents an
Page 104 and 105: 102 6 Reagents and Supplements Cell
Page 106 and 107: 104 6 Reagents and Supplements 6.3
Page 108 and 109: 106 6 Reagents and Supplements 6.5
Page 110 and 111: 108 Content Chapter 7 7 Microbial D
Page 112 and 113: 110 7 Microbial Detection and Elimi
Page 114 and 115: 112 7 Microbial Detection and Elimi
Page 117 and 118: PromoKine 8 Cell Biology Products P
Page 119 and 120: 8 Cell Biology 117 8.1 Cell Analysi
Page 121 and 122: 8 Cell Biology 119 8.7 Cytokines an
Page 123 and 124: Alphabetical Index 121 Description
Page 125 and 126: Alphabetical Index 123 Description
Page 127 and 128: Numerical Index 125 Cat. Number Pro
Page 133 and 134: International Distributors 131 Inte
Page 135 and 136: International Distributors 133 Pola
Page 137 and 138: Terms and Conditions 135 properly p
Page 139 and 140: Fax Order Form Shipping Address Inv
show all

Cell Culture

Create successful ePaper yourself

Delete template?

Save as template?