Cell Culture

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28 1 Human Primary Cells 1.7 Follicle Dermal Papilla Cells Applications Recommended media & reagents Follicle Dermal Papilla Cell Growth Hair growth research Medium (see page 78) 1 Human Primary Cells Drug testing Toxicological testing Features Alkaline phosphatase positive DetachKit (see page 101) Cryo-SFM (see page 85) Related products HFDPC Pellet (see page 97) PromoFectin Transfection Reagent Fig. 1: HFDPC culture in phase contrast. Cryopreserved at second passage (see PromoKine catalog) Available from adult lateral scalp Available from donors with different hair colors Human Follicle Dermal Papilla Cells (HFDPC) are isolated from human der- Sebaceous gland mis originating from the lateral scalp. Information on donor hair and skin color is available for each lot. HFDPC stain Hair shaft Bulge positive for alkaline phosphatase. Dermal papillae are embedded in a laminin and collagen IV rich extracellular Matrix cells matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be Dermal papilla used for in vitro screening of androgen blocking reagents. Fig. 2: Diagram showing the location of the dermal papilla in the hair follicle. https://www.promocell.com/shop Product Size Catalog Number Human Follicle Dermal Papilla Cells (HFDPC) 500,000 cryopreserved cells 500,000 proliferating cells C-12071 C-12072 HFDPC Pellet 1x10 6 cells in RNAlater ® C-14005
1 Human Primary Cells 29 1.8 Hepatocytes Applications metabolism, and albumin synthesis. The Recommended media & reagents cell adherence efficiency is at least 50%. Hepatocyte Growth Medium Drug testing (see page 79) Toxicological testing Metabolism studies Liver physiology research Liver pathology research Oncology The liver is the main metabolic organ in the body performing glycolysis, glycogenesis, amino acid metabolism, lipid metabolism, and ureagenesis. In addition, it also regulates the blood Hepatocyte Maintenance Medium (see page 79) DetachKit (see page 101) Cryo-SFM (see page 85) 1 Human Primary Cells Features sugar level. Furthermore, hepatocytes eliminate toxic substances soon after Related products HHpC Pellet (see page 97) absorption since the nutrition rich blood PromoFectin-Hepatocyte Transfection Cryopreserved immediately after from the gastro-intestinal system first Reagent (see PromoKine catalog) isolation has to pass the liver. This detoxifica- Collagen Type I from Rat Tail 3 - 6 x 10 6 viable cells per cryovial tion consists of phase I and phase II (see page 105) Adherence > 50% reactions. The most important phase I Phase I reaction positive enzyme system is the cytochrome P450 Phase II reaction positive system. Consequently, cultured human Albumin positive hepatocytes are perfectly suited for in vitro metabolism and toxicity/detoxi- Human Hepatocytes (HHpC) are iso- fication studies prior to preclinical or lated from the normal livers of single clinical tests. donors. After isolation, the cells are immediately cryopreserved and then Note: Under standard in vitro cell cul- intensively quality tested. Test cri- ture conditions, mature human hepato- teria are cell viability, cell adherence efficiency, phase I metabolism, phase II cytes usually do not survive for longer than 10-14 days and do not proliferate. Fig 1. HHpC culture in phase contrast. For more information please see www.promocell.com/knowledge-base Count 37,5 Living cells Dead cells Fig. 2: Flow cytometric analysis of PromoCell HHpC using 7-AAD staining. 77 % viable hepatocytes after thawing of the cryopreserved cells. 25 12,5 0 0 10 10 2 10 3 10 4 Cell Lab Quanta SC TM , Beckman Coulter, Inc. 7-AAD https://www.promocell.com/shop Product Size Catalog Number Human Hepatocytes (HHpC) 3 - 6 x 10 6 cryopreserved cells C-12850 HHpC Pellet 1x10 6 cells in RNAlater ® C-14085
Page 1 and 2: Cell Culture 2012
Page 3 and 4: Overview 1 Overview 1 Human Primary
Page 5 and 6: Contents 3 5 6 new Cell Pellets 5.1
Page 7: About PromoCell 5 The PromoCell Cus
Page 10 and 11: 8 Content Chapter 1 1 Human Primary
Page 12 and 13: 10 1 Human Primary Cells 1.2 Chondr
Page 14 and 15: 12 1 Human Primary Cells 1 Human Pr
Page 16 and 17: 14 1 Human Primary Cells Human Pulm
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Page 22 and 23: 20 1 Human Primary Cells Human Brai
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Page 36 and 37: 34 1 Human Primary Cells 1.13 Skele
Page 38 and 39: 36 1 Human Primary Cells Human Coro
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Page 42 and 43: 40 1 Human Primary Cells Overview H
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Page 48 and 49: 46 Content Chapter 2 2 Human Stem a
Page 50 and 51: 48 2 Human Stem and Blood Cells 2 H
Page 52 and 53: 50 2 Human Stem and Blood Cells 2.3
Page 58 and 59: 56 2 Human Stem and Blood Cells Ove
Page 60 and 61: 58 Content Chapter 3 3 Cell Model S
Page 62 and 63: 60 3 Cell Model Systems Cumulative
Page 64 and 65: 62 3 Cell Model Systems Transmigrat
Page 67 and 68: Media and Sera 4 High-quality media
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4 Media and Sera 79 Serum-Free 4.1.
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4 Media and Sera 81 Serum-Free 4.1.
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4 Media and Sera 83 Low Serum 4.1.1
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4 Media and Sera 85 Low Serum 4.1.1
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4 Media and Sera 87 4.2.2 Pericyte
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4 Media and Sera 89 List of supplem
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4 Media and Sera 91 • McCoy´s 5A
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4 Media and Sera 93 MSC-Qualified F
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96 Content Chapter 5 5 Cell Pellets
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98 5 Cell Pellets https://www.promo
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100 Content Chapter 6 6 Reagents an
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102 6 Reagents and Supplements Cell
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104 6 Reagents and Supplements 6.3
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106 6 Reagents and Supplements 6.5
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108 Content Chapter 7 7 Microbial D
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110 7 Microbial Detection and Elimi
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112 7 Microbial Detection and Elimi
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PromoKine 8 Cell Biology Products P
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8 Cell Biology 117 8.1 Cell Analysi
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8 Cell Biology 119 8.7 Cytokines an
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Alphabetical Index 121 Description
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Alphabetical Index 123 Description
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Numerical Index 125 Cat. Number Pro
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International Distributors 131 Inte
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International Distributors 133 Pola
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Terms and Conditions 135 properly p
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Fax Order Form Shipping Address Inv
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Cell Culture

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