Download - R-Biopharm

Food & Feed Analysis
Juni/2007 II/2012
RIDASCREEN ® Tetracyclin
(R3505)
New!
A new test has been developed and is now
available for the screening of milk/milk
powder, dairy products such as cheese,
butter, yoghurt, curd and sour cream,
honey, shrimps and meat including sausage.
This new test kit has the Art. No. R3505
and replaces RIDASCREEN® Tetracyclin,
Art. No. R3503 in our antibiotic ELISA test kit
portfolio.
Evaluation of the new test kit results can be
carried out using RIDA®SOFT WIN version
1.79 onwards. Please contact your local
distributor for the software update if required.
Advantages of the new test system include
enhanced detections limits for most
matrices and a simplified, faster sample
preparation method for meat and the
extended range of matrices.
Detailed information such as validation data
and application notes can be downloaded
at www.celum.r-biopharm.de. Just enter the
PIN Code "ADHLT" in the respective field
and click "PIN Download".
Our products
In the following table, you will find all of the application notes for the RIDASCREEN®
Antibiotic test systems. These are available on request from your local distributor.
Product name Sample Version No.
R1505 - RIDASCREEN® Chloramphenicol Serum / Plasma 06-11-24
R1505 - RIDASCREEN® Chloramphenicol Mixed feed 07-02-15
R1505 - RIDASCREEN® Chloramphenicol Royal Jelly 06-08-09
R1505 - RIDASCREEN® Chloramphenicol Urine 08-12-03
R1505 - RIDASCREEN® Chloramphenicol Yogurt 09-07-31
R1505 - RIDASCREEN® Chloramphenicol Milk Products 09-11-12
R1505 - RIDASCREEN® Chloramphenicol Cheese 09-11-11
R1505 - RIDASCREEN® Chloramphenicol Curd 09-11-18
R1505 - RIDASCREEN® Chloramphenicol Butter 12-03-08
R3103 - RIDASCREEN® Streptomycin Apple Juice / Apple 09-12-01
R3103 - RIDASCREEN® Streptomycin Milk powder 10-11-30
R3103 - RIDASCREEN® Streptomycin Shrimps 11-02-03
R3505 - RIDASCREEN® Tetracyclin Dairy products 11-06-21
R3505 - RIDASCREEN® Tetracyclin Butter 11-06-21
R3505 - RIDASCREEN® Tetracyclin Sausage 11-06-21
R3001 - RIDASCREEN® Sulfamethazin Serum 96-11-24
R3001 - RIDASCREEN® Sulfamethazin Honey 09-07-03
R3703/R3711 - RIDASCREEN® Nitrofuran (AOZ/AMOZ) Honey 09-01-12
R3703/R3711 - RIDASCREEN® Nitrofuran (AOZ/AMOZ) Milk 10-07-19
R3711 - RIDASCREEN® Nitrofuran (AMOZ) Short sample preparation 08-12-11
R3715 - RIDASCREEN® Nitrofuran (SEM) Spiking solution 11-05-26
R3113 - RIDASCREEN® Chinolone/Quinolones Raw Milk 09-06-23
R3113 - RIDASCREEN® Chinolone/Quinolones Honey 10-10-05
Further application notes for
the RIDASCREEN® Mycotoxin,
Hormones & Anabolics and
Allergen range of tests are also
available on request.

RIDASCREEN ® Campylobacter (R4204) – available since May
RIDASCREEN® Campylobacter ELISA
(Art. No. R4204) is now available for
the qualitative detection of Campylobacter
in food samples and can be
ordered from R-Biopharm. The assay
detects C. jejuni and C. coli, the
Campylobacter species responsible
for the majority of food borne
infections, after selective two-stage
pre-enrichment of samples with Bolton
Selective Enrichment Broth.
RIDASCREEN ® Campylobacter
Art. No. R4204
RIDASCREEN® Campylobacter achieves
significantly better recovery rates than
conventional culture on Campylobacter agar,
the method required for culture-based
detection after selective pre-enrichment. This
was confirmed in a national interlaboratory
ring trial in Germany where the study was
designed to validate PCR methods for the
detection of Campylobacter in foods. The
participants however were allowed to use the
RIDASCREEN® Campylobacter ELISA as an
unofficial alternative to the culture-based
method of confirming the test results.
Seven out of the nine laboratories
participating in the study used the ELISA as an
additional confirmatory test. Using 8 positive
and 8 negative ground meat samples each, a
total of 112 samples were tested with the
RIDASCREEN® Campylobacter ELISA. All
negative samples inoculated with E. coli were
confirmed negative by culture as well as by
ELISA. Forty-eight of the 56 positive ground
meat samples inoculated with C. jejuni, were
tested by both culture and ELISA and were
therefore available for comparison.
RIDASCREEN® Campylobacter identified 31 of
the Campylobacter-inoculated samples as
positive whereas culture identified only 21 as
positive. Likewise, the recovery rate was only
43 % for the traditional culture method
compared to 65 % for the immunological
RIDASCREEN® Campylobacter ELISA method.
RIDASCREEN ® Gliadin competitive (R7021) –
Analysis of fermented and hydrolysed food samples
The 2 nd generation RIDASCREEN®
Gliadin competitive ELISA (Art. No.
R7021) has been successfully
introduced into the market and is
used as a method for the analysis of
beer, starch syrup, malt extract and
sourdough. The latest publication
"Gluten Fragment Detection with a
Competitive ELISA" (Haas-Lauterbach et al.:
Journal of AOAC International Vol. 95, No. 2,
2012 377) is available from R-Biopharm.
In cooperation with Prof. Dr. Köhler from the
German Research Center for Food Chemistry,
different beer and starch samples were
analysed with the R5 ELISA RIDASCREEN®
Gliadin competitive. The validation data
shows that the method gives reliable results
for the gliadin or prolamin content of
hydrolysed samples.
RIDASCREEN ® Gliadin competitive
Art. No. R7021
page 2

RIDA ® SOFT Win / RIDA ® SOFT Win.net
An additional version of the RIDA®SOFT Win
(Z9999) software has been developed by
R-Biopharm with the name RIDA®SOFT
Win.net. This version now also supports
64 bit systems however at present this
version is only compatible with a small
selection of ELISA readers. For those
customers, however, who are interested in
this new version and agree to check the
suitability within their daily laboratory
routine, we will gladly allocate this netversion
to them.
Current
version:
1.80
If you are interested in an update of RIDA®SOFT Win,
please contact your local distributor.
Trilogy ® introduces liquid mycotoxin standards
standards. The Trilogy® Liquid Standards can
be used for spiking experiments in order to
check laboratory performance or as calibration
standards for analysis of mycotoxins by
HPLC or GC.
Trilogy® has extended the product portfolio
of mycotoxin standards with liquid mycotoxin
The standards are very easy to use and are
dissolved in organic solvent, as mentioned on
the certificate of analysis and in the table
below.
Article Mycotoxin Concentration Solvent
TSL-104 Aflatoxin B1 25 µg/ml Acetonitrile (10 ml)
TSL-105 Aflatoxin B2 25 µg/ml Acetonitrile (10 ml)
TSL-106 Aflatoxin G1 25 µg/ml Acetonitrile (10 ml)
TSL-107 Aflatoxin G2 25 µg/ml Acetonitrile (10 ml)
TSL-108 Aflatoxins B1, B2, G1, G2
2.0 / 0.5 µg/ml Acetonitrile (10 ml)
(4:1:4:1)
TSL-143 Aflatoxin M1 0.5 µg/ml Acetonitrile (2 ml)
TSL-202 Fumonisin B1, Fumonisin B2
(10:3)
100 / 30 µg/ml Acetonitrile / Water
(50/50) - (2 ml)
TSL-307 Type A & B Trichothecenes 100 µg/ml Acetonitrile (2 ml)
(Fusarenon-X, DON, Nivalenol,
3- / 15-Acetyl-DON, HT-2 Toxin,
Diacetoxyscirpenol, T-2 Toxin,
Neosolaniol)
TSL-314 T-2 Toxin 100 µg/ml Acetonitrile (5 ml)
TSL-317 Deoxynivalenol (DON) 100 µg/ml Methanol (10 ml)
TSL-333 HT-2 Toxin 100 µg/ml Acetonitrile (5 ml)
TSL-401 Zearalenone 25 µg/ml Methanol (10 ml)
TSL-504 Ochratoxin 10 µg/ml Methanol (5 ml)
TSL-601 Patulin 25 µg/ml Acetonitrile (5 ml)
page 3

The standards are stored at 8 °C or at lower
temperature and the shelf life is typically
12 months from the date of manufacture.
Trilogy® Analytical Laboratory provides a wide
range of analytical standards for 30 different
mycotoxins including Fumonisin, Patulin,
Ochratoxin and Trichothecenes. Both the
Trilogy® Dry Mycotoxin Standards and the
Trilogy® Liquid Mycotoxin Standards are
almost ready to use, have a long shelf life and
reduce hazardous handling with toxins. They
are intended for use by customers who do
not have a spectrophotometer or for those
who want to ensure accurate HPLC
determination of mycotoxins with minimal
preparation and effort.
In addition to generating calibration curves,
both types of the Trilogy® standards can also
be used to spike samples.
Trilogy® provides with each standard a
Certificate of Analysis (COA) stating the
concentration, uncertainty, manufacturing
date and solvent to be used for reconstitution
for the dry standards. All certificates and
MSDS are available online:
www.trilogylab.com
SureFood ® PCR products from our partner
CONGEN Biotechnologie GmbH, Berlin
The SureFood® GMO portfolio is continuously
adapting in response to current issues.
New additions include kits for the qualitative
detection of specific GMO events. Like the
existing detection kits, they are supplied in a
100-reaction format that can be employed in
modular fashion.
Samples to be subjected to GMO testing are
usually first tested for the presence of GMOs
after DNA preparation. This is done with
the help of screening tests such as
SureFood® GMO Screen 4-Plex
35S/NOS/FMV+IAC (S2126).
With the specific test kit, positive
samples can be identified on the
FAM channel of almost all commercially
available real-time thermal
cyclers.
New additions:
S2034 SureFood® GMO ID RR2Y Monsanto, herbicide resistance in soybeans
S2035 SureFood® GMO ID MIR162 Corn Syngenta, insecticide resistance in corn
S2037 SureFood® GMO ID MON863 Corn Monsanto, insecticide resistance in corn
S2039 SureFood® GMO ID MIR604 Corn Syngenta, insecticide resistance in corn
S2060 SureFood® GMO ID T45 Canola Bayer CropScience, herbicide resistance
in canola
S2058 SureFood® GMO ID GT73 Canola Monsanto, herbicide resistance in canola
In the latter case of herbicide resistance in
canola, a quantitative assay for 2 x 50
reactions (50 for the target gene and 50 for
the reference gene quantification) is also
available. This assay makes it possible to
determine what labeling is needed for food
products containing GMO event GT73, the
importation of which is approved under
current EU law, in cases where relative
amounts exceed 0.9 % per total composition:
S2061 SureFood® GMO QUANT GT73 Canola Monsanto, herbicide resistance in canola
If you are interested in our products,
please contact your local distributor.
page 4

Information from R-Biopharm Rhône,
Scotland
New publications
R-Biopharm Rhône Ltd (RBR) is continually
sourcing papers which reference the use of
the R-Biopharm products in order to provide
information to the customers and also to
further improve and develop the products.
As a result of this, a number of new papers
have been added to the RBR key publication
library including the following:
• The use of AFLAPREP® in the analysis of
sesame seeds
• The use of RIDASCREEN® Zearalenon in the
analysis of bovine urine
• The analysis of Aflatoxin M1 in goats milk
using RIDASCREEN® Aflatoxin M1
• EASI-EXTRACT® Aflatoxin for the analysis of
aflatoxins in spices and medicinal plants
• AFLAPREP® M1 and the analysis of milk
using TLC/HPLC
These papers act as excellent references of
people and institutions using the R-Biopharm
products for the analysis of a wide variety of
commodities.
New format of the Instructions for Use
R-Biopharm Rhône Ltd is currently working
through the process of updating all of our
instructions for use into a new A5 booklet
format. They have been designed to be more
user friendly for the operator by condensing
all of the information into one handy size.
The instructions will contain the primary
methods for the main commodities associated
with that mycotoxin however application
notes for more complex matrices will still be
available on request.
AO ZON PREP ® Immunoaffinity columns
(Art No.: RBRP112, RBRP112B)
R-Biopharm Rhône Ltd was proud to
announce the launch of AO ZON PREP® in
February of this year. It has extended the
range of multi-toxin immunoaffinity columns
in the range and is already proving to be a
successful product. The column uses one
common extraction for the analysis of total
aflatoxin, ochratoxin A and zearalenone.
The use of AO ZON PREP® offers analysts an
easy way to extract, purify and selectively
concentrate total aflatoxin, ochratoxin A and
zearalenone from a wide range of food and
feed commodities. This will then allow
optimum detection by HPLC or LC-MS/MS.
Currently an application note is available for
beer and the technical services department is
also working on methods for baby food and
animal feed.
page 5

EASI-EXTRACT ® VITAMIN B12 (LGE) recieves
AOAC Official First Action Status
Now also EASI-EXTRACT® VITAMIN B12 (LGE),
Art. No. RBRP88/RBRP88B has recently been
accepted by AOAC International as an Official
First Action method. The method for the
detection of vitamin B12 in infant formula and
adult nutritionals in conjunction with
UV-HPLC was assigned Official Method SM
number 2011.08. The results of the
collaborative study will be published in the
AOAC Journal. This will be published as part
of a collaborative study organized by Nestec,
Switzerland and will be included in the
"Official Methods of Analysis" online at
www.eoma.aoac.org and in the next printed
edition.
Related products for
Vitamin B12 analysis are:
Product
Art. No.
Immunoaffinity columns
EASI-EXTRACT® Vitamin B12 RBRP80/80B
Microbiological Microtiter plate assay
VitaFast® Vitamin B12 P1002
ELISA, Microtiter plate
RIDASCREEN®FAST Vitamin B12 R2102
Fairs and Conferences Representative: R-Biopharm AG
24. - 28.06.2012 MYCORED North America 2012
Carleton University in Ottawa, Canada
30.09. - 03.10. 2012 AOAC Annual Meeting
Planet Hollywood
Las Vegas, Nevada, USA
30.09. - 03.10.2012 AACCI Annual Meeting
Hollywood, Florida, USA
The next R-Biopharm news will be published in the III rd quarter 2012.
R-Biopharm news is edited by
R-Biopharm AG
An der neuen Bergstraße 17
64297 Darmstadt, Germany
Reg.-Nr.: Amtsgericht Darmstadt, HRB 8321
Phone: +49 (0) 61 51 - 81 02-0
Fax: +49 (0) 61 51 - 81 02-40
E-mail: info@r-biopharm.de
www.r-biopharm.com