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www.mpbio.com<br />

CATALOG<br />

NUMBER<br />

CELLAGEN MEMBRANES<br />

A Permeable Collagen Film for Tissue Culture<br />

Cellagen Membranes are permeable collagen films developed for tissue<br />

culture use that permit the characteristic permeation of substances such<br />

as absorption of nutrients and exhaustion of metabolites through the<br />

membrane. Cellagen Membranes are especially effective on epithelial<br />

cell culture since they allow the best utilization of cell polarization.<br />

Also, long-term cultures can be done in keeping with the differentiated<br />

phenotypes in the living body. Additionally, since two types of cells can<br />

be cultured on both sides of the membrane, Cellagen may be used<br />

to study cell-cell interactions and for the basic study of hybrid artificial<br />

organs.<br />

Cellagen Membranes are made of highly purified, pyrogen-free, pepsinsolubilized<br />

collagen. The membranes are transparent, permitting easy<br />

observation of the cultured cells <strong>by</strong> phase contrast microscopy.<br />

Each set of membranes contains 5-discs with 33 mm diameter membrane,<br />

membrane bracket and culture dish.<br />

Ref.:Carney, E.W. et. al., Co-Culture of Rabbit One-Cell Embryos With<br />

Rabbit Oviduct Epithelial Cells, In Vitro Cell. Dev. Biol., 26: 629-635<br />

(1990).<br />

-20°C<br />

152299 5/set<br />

CELLAGEN SHEET<br />

Cellagen Sheets are Cellagen Membranes in a 9 x 10 x 35m sheet<br />

which can be cut in any custom shape or size.<br />

RT<br />

152399 1 sheet<br />

CELLAGEN SOLUTION EMEM<br />

A 0.2% solution of pepsin solubilized type I collagen in Eagle MEM<br />

substrate, pH 7.4.<br />

0 TO 5°C<br />

152396 20 ml<br />

CELLAGEN SOLUTION HANKS<br />

A 0.2% solution of pepsin solubilized type I collagen in Hanks substrate,<br />

pH 7.4.<br />

0 TO 5°C<br />

152397 20 ml<br />

CELLAGEN SOLUTION PC-3<br />

A 0.3% solution of pepsin solubilized type I collagen, pH 3.0.<br />

0 TO 5°C<br />

152391 25 ml<br />

100 ml<br />

Biochemicals<br />

Alphabetical List<br />

CELLAGEN DISCS CD-24<br />

Permeable collagen film for cell culturing, designed for use in 24-well<br />

culture plates. 14 mm diameter membranes in brackets. Protocol for use<br />

available. Also see Cellagen Membranes.<br />

-20°C<br />

152325<br />

152316<br />

24 pcs.<br />

48 pcs.<br />

CATALOG<br />

NUMBER<br />

CELLAGEN SOLUTION PC-5<br />

A 0.5% solution of pepsin solubilized type I collagen, pH 3.0.<br />

0 TO 5°C<br />

152392 100 ml<br />

CELLAGEN SPONGE 35<br />

Uniquely developed collagen specially prepared for 3-dimensional tissue<br />

culture studies. Porous collagen structure allows cells to penetrate into<br />

the pores, permitting a 3-dimensional approach to tissue culture. The<br />

cellagen sponge is also applicable in high density culture studies.<br />

32 mm diameter, 1mm thick fits 35mm culture dishes.<br />

One package contains 5 sponges.<br />

RT<br />

158223 1 pkg<br />

CELLAGEN SPONGE 60<br />

Uniquely developed collagen specially prepared for 3-dimensional tissue<br />

culture studies. Porous collagen structure allows cells to penetrate into<br />

the pores, permitting a 3-dimensional approach to tissue culture. The<br />

cellagen sponge is also applicable in high density culture studies.<br />

48 mm diameter, 1mm thick fits 60 mm culture dishes.<br />

One package contains 5 sponges.<br />

RT<br />

158224 5 pk<br />

CELLAGEN SPONGE 100<br />

Uniquely developed collagen specially prepared for 3-dimensional tissue<br />

culture studies. Porous collagen structure allows cells to penetrate into<br />

the pores, permitting a 3-dimensional approach to tissue culture. The<br />

cellagen sponge is also applicable in high density culture studies.<br />

83 mm diameter, 1mm thick fits 100 mm culture dishes.<br />

One package contains 5 sponges.<br />

RT<br />

158225 5 pk<br />

CELLFORM POLYMER<br />

(Poly[2-hydroxyethyl methacrylate])<br />

A biocompatible polymer, readily soluble in alcohol/water solutions.<br />

Forms a non-adhesive cell culture coating that controls cell shape <strong>by</strong><br />

diminishing substrate adhesiveness. Useful for study of cell growth<br />

control and sensitivity to changes in shape. Also used for cell adherence<br />

studies and analysis of relationships between cell spreading and cellular<br />

metabolism.<br />

RT<br />

150207 1 g<br />

5 g<br />

CELLOBIOHYDRALASE<br />

(1,4--D-Glucan Cellobiohydralase, EC 3.2.1.91)<br />

From: Geotrichium candidum<br />

Chromatographically purified, lyophilized powder.<br />

Contains less than 0.2% carbohydrase activity.<br />

Activity: 6 U/mg<br />

Unit Definition: One unit of activity is defined as the amount of enzyme<br />

that liberates 1mole of reducing sugars per minute from amorphous<br />

cellulose at 30°C, pH 5.0.<br />

198900 500 U<br />

www.mpbio.com To place an order 1.800.854.0530, fax 1.800.334.6999<br />

131

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