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CATALOG<br />

NUMBER<br />

AURORA SOUTHERN BLOT KIT For<br />

Chemiluminescent Nucleic Acid Detection<br />

Capacity: 10-20 blots or 50-100 blots<br />

(10x10 cm membrane)<br />

The Aurora Southern Blot Kit is a highly sensitive and versatile<br />

NON-isotopic chemiluminescence-based system for the detection<br />

of biotinylated nucleic acids in Southern and Northern blotting. This<br />

system offers convenience and speed over isotopic methods <strong>by</strong> utilizing<br />

the StarLight high performance chemiluminescent substrate and<br />

ActiBind-AP streptavidin-alkaline phosphatase conjugate for maximum<br />

signal intensity and low background results. As little as 1 pg of hybridized<br />

DNA and even sub-picogram quantities of biotinylated DNA bound to the<br />

membrane have been detected. Exposures require less than 60 minutes<br />

on either standard X-ray or instant film. The chemiluminescence emission<br />

will last for several days permitting multiple exposures and easy stripping<br />

and reprobing.<br />

Kit Contents:<br />

• StarLight substrate<br />

• ActiBind-AP<br />

• Aurora Blocking reagent<br />

• Diethanolamine<br />

A complete protocol booklet is supplied with each kit.<br />

See also Biotrans Nylon and PVDF Transfer Membranes.<br />

0 TO 5°C<br />

821559 10 blot<br />

AURORA WESTERN BLOT KIT (MOUSE) For<br />

Chemiluminescent Immunoblotting<br />

The Aurora Western Blot Kit is a versatile, complete non-isotopic<br />

chemiluminescent immunoblotting system. The kit provides<br />

advantages of speed, sensitivity, flexibility, and control of film exposure<br />

time. The Aurora system utilizes the StarLight high-performance<br />

chemiluminescent substrate for alkaline phosphatase to detect a<br />

secondary antibody-alkaline phosphatase conjugate for extremely<br />

sensitive detection of proteins.<br />

This specific kit utilizes a goat anti-mouse IgG/IgM-AP conjugate as<br />

secondary antibody.<br />

Kit Capacity: 10 or 30 blots (10cm x 10cm).<br />

Kit Contents:<br />

•StarLight substrate<br />

•Secondary antibody conjugate<br />

•Aurora Blocking reagent<br />

•Opti-Membrane Reagent<br />

•Diethanolamine<br />

A complete protocol is supplied with each kit.<br />

0 TO 5°C<br />

821536<br />

821537<br />

Biochemicals<br />

Alphabetical List<br />

10 blot<br />

40 blot<br />

CATALOG<br />

NUMBER<br />

AURORA WESTERN BLOT KIT (RABBIT) For<br />

Chemiluminescent Immunoblotting<br />

The Aurora Western Blot Kit is a versatile, complete non-isotopic<br />

chemiluminescent immunoblotting system. The kit provides<br />

advantages of speed, sensitivity, flexibility, and control of film exposure<br />

time. The Aurora system utilizes the StarLight high-performance<br />

chemiluminescent substrate for alkaline phosphatase to detect a<br />

secondary antibody-alkaline phosphatase conjugate for extremely<br />

sensitive detection of proteins.<br />

This specific kit utilizes a goat anti-rabbit IgG-AP conjugate as<br />

secondary antibody.<br />

Kit Capacity: 10 or 30 blots (10cm x 10cm).<br />

Kit Contents:<br />

•StarLight substrate<br />

•Secondary antibody conjugate<br />

•Aurora Blocking reagent<br />

•Opti-Membrane Reagent<br />

•Diethanolamine<br />

A complete protocol is supplied with each kit.<br />

0 TO 5°C<br />

821538 10 blot<br />

821539 40 blot<br />

AVERMECTIN B1<br />

(Abamectin; AVM)<br />

A macrocyclic lactone with anthelmintic and pesticidal properties.<br />

Avermectins interact with GABA receptors.<br />

C48H72O14<br />

www.mpbio.com<br />

0 TO 5°C MW 873.1 71751-41-2<br />

195891 10 mg<br />

50 mg<br />

AVIDIN<br />

Activity: 10 units/mg<br />

Unit Definition: One unit will bind 1g of D-biotin, pH 8.9; glycoprotein<br />

with tetrameric structure.<br />

-20°C 1405-69-2<br />

100303 100 U<br />

1 KU<br />

AVIDIN<br />

From: Egg White<br />

Freeze-dried<br />

Activity: 10-15 units/mg solid<br />

Unit Definition: One unit is that amount of protein that will bind 1g<br />

of D-biotin.<br />

Very high affinity for biotin, useful in a biotin-avidin system for labeling<br />

biomoleculesforreceptorstudies,immunoassaysandimmunohistological<br />

methods.<br />

0°C<br />

150047 5 mg<br />

10 mg<br />

25 mg<br />

100 mg<br />

84<br />

One Call. One Source. A World of Biotechnology Reagents. www.mpbio.com

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