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Skin Biomarkers as objective measures of stratum corneum barrier

Skin Biomarkers as objective measures of stratum corneum barrier

Skin Biomarkers as objective measures of stratum corneum barrier

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Biomarker Sampling and Analysis Approach<br />

“Non-inv<strong>as</strong>ive sampling <strong>of</strong> <strong>stratum</strong> <strong>corneum</strong>”<br />

Sampling Sites<br />

● Leg<br />

● Arm<br />

● Targeted Multiple Analyte Assay<br />

- Multiplexed ELISA<br />

- LC/MS/MS<br />

● Colorimetric Assay for Protein<br />

- normalize targeted analyte data<br />

1) Apply D-Squame Tape 2) Remove Tape 3) Extract Analytes 4) Analysis Samples

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