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15% Hanks balanced salt solution, 75 μg/ml methionine and 1.57 mg/ml glucose. At the end of the culture period, growth and differentiation of each embryo was evaluated by means of a morphological scoring system. Albendazole caused dysmorphogenic development of the rat embryos at a concentration of 0.5 mg/L, the sulfoxide was less toxic and caused abnormalities at a concentration of 2.5 mg/L and higher. The 50:50 combination of both substances resulted in malformed embryos at concentrations of 1.0 mg/L and more. The effects on the rat embryos manifest as generally underdeveloped with a concentration-dependent decrease in protein content, crown-rump length and morphological score. In conclusion, by using the rat whole-embryo-culture system we showed that albendazole exhibits a pronounced embryotoxic potential and is more potent (approximately 5-fold) than its metabolite albendazole sulfoxide. 405 EMBRYOTOXIC POTENTIAL OF MYCOPHENOLIC ACID IN TWO IN VITRO TESTS. K. Eckardt, A. Felies and R. Stahlmann. Institute for Clinical Pharmacology and Toxicology, Charite - Universitätsmedizin Berlin, Berlin, Germany. Mycophenolate mofetil is a widely used immunosuppressive drug which recently has been categorized as a human teratogen. So far it has not been studied in embryotoxicity in vitro assays. Therefore, we evaluated the embryotoxic potential of mycophenolic acid in two validated in vitro assays: the rat whole embryo culture and the murine embryonic stem cell test. Rat embryos cultured from gestational day 9.5 for 48 hours with the drug showed dysmorphogenic development at concentrations of 250 μg mycophenolic acid/l medium and higher. At concentrations of 750 μg/l and more all rat embryos exhibited malformations. The main effects were defective yolk sac blood circulation, neural tube defects, malformations of the head and heart anomalies. The exposed embryos showed a concentration-dependent decrease in protein content, crown-rump length, number of somites and morphological score. The murine embryonic stem cell test was slightly more sensitive. Proliferation and differentiation of the ES-D3-cells were significantly impaired at concentrations of 31 and 125 μg mycophenolic acid / l medium, respectively. In the differentiation assay, at a concentration of 125 μg mycophenolic acid / l medium and more, the number of wells with differentiated cardiomyocytes significantly decreased. Additionally, a cytotoxicity assay with balb/c 3T3 mouse fibroblasts was used to compare the proliferation and vitality of embryonic cells with adult cells. In the balb/c 3T3 cytotoxicity assay the number of vital mouse fibroblasts significantly decreased at a mycophenolic acid concentration of 62 μg/l and more. In conclusion, by using two validated in vitro tests we showed that mycophenolic acid exhibits a pronounced embryotoxic potential at cytotoxic concentrations. This result supports the use of these tests to detect human teratogens. 406 KISSPEPTIN: A NEW PATHWAY TO IDENTIFY AND CLASSIFY ENDOCRINE DISRUPTING COMPOUNDS? S. Losa, H. B. Adewale, A. W. Sullivan, J. A. Mickens, K. L. Todd and H. B. Patisaul. Biology, North Carolina State University, Raleigh, NC. Sponsor: G. LeBlanc. There is mounting evidence that the incidence of precocious puberty, unexplained infertility and other adverse female reproductive health effects is increasing. It has been proposed that this is due, at least in part, to exposure to endocrine disrupting compounds (EDCs). The specific mechanisms by which EDCs impact female reproductive health are not yet fully understood and consensus on an adequate method for predicting EDC effects in humans has not been reached. Here we show that disruption of hypothalamic kisspeptin signaling pathways is associated with advanced puberty and premature anestrous, making this system a potentially good biomarker for human endocrine disruption. Kisspeptins (KISS) are a newly discovered family of peptides that, through the kisspeptin receptor, act directly on gonadotropin releasing hormone (GnRH) neurons to regulate GnRH secretion. Initially discovered in humans, similar systems have now been well characterized in other mammalian species. In the murine brain, there are two major populations of KISS neurons: one surrounding the anterior periventricular nucleus (AVPV) and one in the arcuate (ARC). KISS neurons in the AVPV are colocalized with both ERα and ERβ, while the ARC KISS neurons express only ERα. Only the AVPV population is sexually dimorphic with females having more KISS neurons than males. Here we show that neonatal administration of estradiol benzoate (EB) to female rats reduces KISS immunoreactivity (-ir) in both the AVPV and ARC. We further show that selective agonism of ERα, but not ERβ, can best replicate the defeminizing impact of estrogen. Finally, we assessed the impact of neonatal exposure to genistein (a phytoestrogen), or bisphenol-A (BPA, a plastics component) on KISS-ir in the AVPV and ARC and found that each could produce region specific defeminizing effects. We propose that examination of KISS signaling pathways could provide valuable information about a compound’s potential endocrine disrupting properties as well as insight into whether the mechanistic pathway is through ERα or ERβ. 407 MALE REPRODUCTIVE PARAMETERS IN A RAT TWO- GENERATION REPRODUCTION STUDY OF AMMONIUM PERFLUOROOCTANOATE. R. G. York 1 , G. L. Kennedy 2 , G. W. Olsen 3 and J. L. Butenhoff 4 . 1 WIL Research Laboratories, LLC, Manlius, NY, 2 DuPont Company, Newark, DE, 3 Medical Department, 3M Company, St. Paul, MN and 4 Medical Department, 3M Company, St. Paul, MN. Perfluorooctanoate (PFOA) is an industrial surfactant used in the manufacture of fluoropolymers. The environmental and metabolic stability of PFOA together with its presence in human blood and long elimination have led to extensive toxicological studies. Two recent publications based on observations from the Danish general population have reported a negative association between serum PFOA levels in young adult males and their sperm counts; and a positive association among women with time to pregnancy. A rat reproduction study was published (2004) in which no effects on reproduction were observed at doses up to 30 mg PFOA/kg body weight. In order to place the recent human epidemiological data in perspective, we are providing the detailed male reproductive parameters from the two-generation study, including sperm quality and testicular histopathology. Sperm parameters from the two-generation study in all PFOA treatment groups were normal and reflected the normal fertility observations in these males. No evidence of altered testicular and sperm structure and function was observed at mean group serum PFOA concentrations ranging up to approximately 50,000 ng/mL. Evidence from workers, where mean PFOA levels ranged from two to four orders of magnitude higher than concentrations found in the general population, did not show any consistent changes in reproductive hormonal levels that could be associated with a decrease in reproductive function. In addition, male monkeys given daily capsules containing PFOA for six months had no alterations in testicular structure or sex hormones at group mean serum concentrations ranging up to 110,000 ng/mL. Given that median serum PFOA in the Danish cohorts was approximately 5 ng/mL, it seems unlikely that levels observed in the general population, including those recently reported in Denmark, could be associated with a real decrease in sperm. 408 ESTROGEN LIKE EFFECTS OF CADMIUM IN MICE: ARE THEY MEDIATED VIA NUCLEAR ESTROGEN RECEPTOR SIGNALING? I. Ali 1 , P. E. Penttinen Damdimopoulou 2 , S. I. Mäkelä 2, 3 , M. Berglund 1 , U. Stanius 1 , A. Åkesson 1 , H. Håkansson 1 and K. Halldin 1 . 1 Institute of Environmental Medicine, Karolinska institute, Stockholm, Sweden, 2 Functional Food Forum, University of Turku, Turku, Finland and 3 Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland. Cadmium (Cd) is a ubiquitous toxicant of environmental concern. At environmentally relevant doses, Cd has been shown to induce several well-characterized in vivo estrogenic responses like increased uterine weight, hyperplasia and hypertrophy of endometrial lining and induction of uterine progesterone receptor expression. Several in vitro studies suggest that Cd-induced estrogenic effects may be mediated via estrogen receptors (ERs). However, it is unclear whether observed effects are mediated via classical ER-signaling or via other intracellular signaling pathways. We investigated the estrogenic effects in relation to CdCl2 exposure using ERE-luc mice. Immature mice were subcutaneously exposed to 5, 50 & 500μg CdCl2/kg b.w., sterile PBS or EE2 for 3-consecutive days. Onset of puberty (vaginal opening), body and organ weight, histology of uterine, Cd tissue retention and luciferase expression was analyzed. We found that EE2 significantly increased the uterine wet weight as well as luciferase expression in various tissues along with early onset of puberty. Cd did not significantly increase the uterine wet weight in any of the dose groups. No onset of puberty was observed. Luciferase expression data showed no clear effects either in reproductive or in non-reproductive organs after CdCl2 treatment. Interestingly, height of the uterine epithelium was significantly increased among EE2 as well as CdCl2 treated groups. Western blot analysis showed a dose dependent activation of MDM2; however ERK was highly activated only in low dose CdCl2 group. In conclusion, our data suggest that Cd can exert some estrogen-like-effects in immature female mice at the doses investigated; however the effects are not likely to be mediated via nuclear estrogen receptors. 409 BACKGROUND DATA FROM DEVELOPMENTAL TOXICITY STUDIES IN THE RABBIT. P. C. Barrow, J. Briffaux, L. Allais, M. Leroy and J. Guyot. MDS Pharmacology Services, Saint-Germain sur l’Arbresle, France. The rabbit may be chosen as an animal model in developmental toxicity studies, which are particularly conducted for the development of vaccines intended for use in women of childbearing potential and adolescents (FDA Guidance for Industry, 88 SOT 2010 ANNUAL MEETING
February 2006). The rabbit is one of the species known for its immunogenic response to human vaccines and its efficient transfer of maternal antibodies to the fetuses or kits through the placenta or milk. Postnatal studies have rarely been performed in the rabbit, mainly due to the notorious practical difficulties of handling rabbit litters without provoking maternal cannibalism. If, however, these difficulties could be overcome, the rabbit would be an ideal species for the developmental toxicity testing. For vaccine studies, treatment has to start before mating in order to ensure adequate antibody titers during early gestation. At least 13 studies were conducted using optimal housing and handling conditions for the rabbit does and offspring during the lactation period. Human contact was limited during the early phase of lactation in order to minimize interferences with nursing behavior. Litter parameters, including the number of kits born, kit survival, kit weights and developmental milestones (including fur growth, incisor eruption and eye opening) and functional tests were recorded up to weaning. The background data generated at the Testing Facility could serve as a basis for future developmental studies in this species and confirmed that the rabbit can be used as a model for postnatal developmental toxicity studies. Based on discussions with the regulatory authorities for the development of vaccines, we currently aim to evaluate 20 litters per group for each of the prenatal and postnatal phases. In order to achieve these numbers, 24 and 30 females per group are used for the prenatal and postnatal phases, respectively. Blood samples can also be taken from dams, fetuses and kits to assess the immunogenic response and antibody transfer through the placenta and milk. 410 TESTICULAR GENE EXPRESSION PROFILING FOLLOWING LACTATIONAL EXPOSURE TO 6-N- PROPYLTHIOURACIL (PTU), A NEONATAL GOITROGEN. M. L. Hixon 1 , J. Santos Ahmed 1 , A. DeLong 2 , C. Brown 1 , S. Duncan Smith 1 , T. Rasoulpour 1 , C. Schorl 3 and Z. Wu 2 . 1 Department of Pathology, Brown University, Providence, RI, 2 Department of Community Health, Brown University, Providence, RI and 3 Center for Genomics and Proteomics, Brown University, Providence, RI. PKBα/Akt1 regulates germ cell proliferation and apoptosis. Therefore, we hypothesized that lack of Akt1 may alter Sertoli cell proliferation of the postnatal testis following exposure to 6-N-propylthiouracil (PTU) which extends Sertoli cell proliferation resulting in larger testis size. To test this, we examined the requirement of Akt1 following lactational exposure to PTU (0.01%). In adult Akt1+/+ and Akt1+/- mice, testis weight and daily sperm production were significantly increased following PTU exposure. Akt1-/- mice exposed to PTU exhibited testes weight and daily sperm production similar to those of control Akt1-/- mice. At postnatal day 25 (PND25), histological analyses of PTU-exposed Akt1-/- seminiferous tubules demonstrated increased vacuolization and germ cell apoptosis relative to PTU-exposed Akt1+/+ animals. To elucidate gene changes mediating these phenotypic effects, we monitored changes in gene expression at PND25 in Akt1+/+, Akt1+/-, and Akt1-/- testes following transient hypothyroidism. Postnatal exposure significantly altered over 20,000 transcripts in Akt1-/- males relative to postnatal exposure in Akt1+/+ and Akt1+/- males. Gene ontology revealed that PTU targets proteins that make up the testicular tight junctions, including members of the claudin gene family. The transcriptional profile suggests gene changes associated with formation of the blood-testis barrier and indicates that loss of Akt1 in the testis impairs the timing of testicular tight junction formation and consequently the functional integrity of the testis following toxicant insult. 411 CHARACTERIZATION OF P-GLYCOPROTEIN (ABCB1) ORTHOLOGS IN THE RAT EPIDIDYMIS: UNIQUE DISTRIBUTION AND UP-REGULATION BY XENOBIOTICS. S. R. Jones and D. Cyr. Institut Armand-Frappier, INRS, Laval, QC, Canada. Sponsor: B. Hales. The epididymis and maturing sperm are susceptible to xenobiotics. The blood-epididymis barrier is necessary to attenuate the entry, and elimination of harmful substances. In addition to providing an immunological barrier, the epididymis also provides a specialized environment necessary for sperm maturation. The epididymal epithelium contains numerous specialized transporters that selectively regulate compounds present in the epithelium and lumen. abcb1a and abcb1b are rodent orthologs of human P-glycoprotein (ABCB1), a member of the ATP-binding cassette (ABC) efflux proteins capable of regulating the excretion of xenobiotics in normal tissues. The objective of this study was to characterize the previously unknown expression profile, localization and inducibility of abcb1a and abcb1b in the rat epididymis. abcb1a mRNA levels were significantly higher in the cauda epididymidis when compared to initial segment, caput and corpus. abcb1b mRNA levels were similar throughout the epididymis. Immunohistochemistry using antisera against both forms of abcb1 indicated minimal immunostaining in the epithelial cells or spermatozoa in the caput epididymidis, and progressively increased in the corpus and cauda. Interestingly, the immunoreaction in spermatozoa was detected in the distal caput, corpus and cauda. This was confirmed by western blot analysis. To assess if abcb1 was inducible by xenobiotics, rat epididymal cells (RCE) were exposed to different concentrations of nonylphenol (NP) or doxorubicin (DOX). RCE cells exposed to 20 uM NP and 500 ng/ml DOX revealed a significant induction of both abcb1a and abcb1b mRNA and abcb1 total protein, suggesting that ABC efflux transporters are inducible in the epididymis. The unique expression profile and induction of abcb1a and abcb1b in the epididymis suggests an important role for these proteins as a defence mechanism against xenobiotics in the epididymis. Supported by Environment Canada, CIHR and Armand-Frappier Foundation. 412 ASSESSMENT OF ORGANOTYPIC EPIVAGINAL TM TISSUE MODEL TO SCREEN IRRITATION POTENTIAL OF CHEMICALS. C. Cannon, S. Ayehunie, K. LaRosa and M. Klausner. MatTek Corp, Ashland, MA. A predictive test system for assessing the vaginal irritation of chemicals and formulations will have far reaching application in industries involved in feminine care products. The vaginal mucosa is commonly exposed to chemicals or therapeutic agents that can cause irritation/inflammation and increase the susceptibility to infections such as HIV-1 and HSV-2. Hence, chemical/formulation or therapeutic agent induced vaginal irritation is a concern for industrial and academic toxicologists. Traditionally, testing has been performed using the rabbit vaginal irritation (RVI) assay. In the current study, we investigated use of the organotypic, highly differentiated EpiVaginal tissue as a non-animal alternative. EpiVaginal tissues were exposed to N=6 chemicals at 3 concentrations for 1, 3, and 6 hrs. The effects of single or repeat application on tissue viability (MTT assay), barrier disruption (measured by trans-epithelial electrical resistance, TEER, or sodium fluorescein, FL, leakage), and inflammatory cytokine release (IL-1a, IL-1b, IL-6, and IL-8) were examined. When compared to untreated controls, two irritating test articles, benzalkonium chloride and nonoxynol-9, reduced tissue viability to
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The Toxicologist Supplement to Toxi
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The Toxicologist Supplement to Toxi
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1 BIOLOGICAL PATHWAY ANALYSIS: AN I
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11 ICH INITIATIVES FOR CONDUCTING P
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models. Experimental approaches and
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30 SILICA AND ASBESTOS IMMUNOTOXICI
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40 NONCANCER TOXICITY POTENTIAL AT
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ased products for patient administr
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nase regulates Hsp27-induced reorga
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exposure resulted in splenomegaly.
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We investigated the effect of imati
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well plate prevented free cell move
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98 DERIVING SIGNATURES OF IN VIVO T
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sulfate, cinnamic aldehyde, 2,4-din
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The final product will be a system
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mercially available STP brands by m
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for six weeks, with 10 mg/kg azoxym
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eceived RES post-TCDD exposure when
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morigenesis. Knocking down of JARID
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163 MICROPET IMAGING OF [18F]-DFNSH
Page 41 and 42: These results are comparable to tha
Page 43 and 44: activity as assessed by lever press
Page 45 and 46: for measured physico-chemical param
Page 47 and 48: 199 DEVELOPMENT OF A MULTIPARAMETER
Page 49 and 50: To cause PLD, a drug needs to enter
Page 51 and 52: In contrast to the parent PBDEs and
Page 53 and 54: transiently transfected HEK 293 cel
Page 55 and 56: TCDD exposure, 24 h prior to a 20 %
Page 57 and 58: 244 NON-ADDITIVE EFFECTS OF PCB153
Page 59 and 60: 253 COMPARISON OF MIXTURE TOXICITY
Page 61 and 62: two cerium oxide nanoparticles of v
Page 63 and 64: 271 SIZE-DEPENDENT EFFECTS OF TUNGS
Page 65 and 66: adigms such as Tox 21 and Toxcast,
Page 67 and 68: QDs with emission maximum at 800nm
Page 69 and 70: Cleveland, while others were found
Page 71 and 72: without the need of animal testing.
Page 73 and 74: Conclusions: These results are cons
Page 75 and 76: ing oxime reactivation and organoph
Page 77 and 78: 335 A NON-OXIME IMPROVES SURVIVAL I
Page 79 and 80: alties suffer from permanent lung i
Page 81 and 82: ies have established inflammatory b
Page 83 and 84: 363 GENETIC VARIATION IN ISOGENIC M
Page 85 and 86: 372 EVALUATING THE BIOLOGICAL INTER
Page 87 and 88: dose of 1/20 LD50 (400 mg/kg.bwt) f
Page 89 and 90: median CR-adjusted isoflavone conce
Page 91: data indicate that AhR deletion pro
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Page 97 and 98: of selected microorganisms that are
Page 99 and 100: BVI. Histopathological analysis was
Page 101 and 102: 446 MOLECULAR CLONING AND CHARACTER
Page 103 and 104: portant in predicting risk. CYPs 1A
Page 105 and 106: ats, which involves metabolic activ
Page 107 and 108: 473 BOVINE CORNEAL OPACITY AND PERM
Page 109 and 110: 482 TYPE III DEIODINASE (D3) IS PRO
Page 111 and 112: tancy evaluation and ranking of bat
Page 113 and 114: 501 CHARACTERIZATION OF ESTERASE, G
Page 115 and 116: 510 REDOX CYCLING BY ENDOGENOUS 2-
Page 117 and 118: prostate cancer cells. All 8 BEL de
Page 119 and 120: metallic nickel nanoparticles. Acti
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Page 123 and 124: 550 QUALIFICATION STRATEGIES-GENOTO
Page 125 and 126: ferentiation, and 3) how mixtures o
Page 127 and 128: 572 LOW-CHRONIC ARSENIC EXPOSURE: E
Page 129 and 130: 582 IDENTIFICATION OF SENSITIVE URI
Page 131 and 132: duced by the genetic outcross of fe
Page 133 and 134: fects of new compounds are equally
Page 135 and 136: acterize the current state of the s
Page 137 and 138: 620 CHARACTERIZATION OF POTENTIAL I
Page 139 and 140: ways. Moreover, both MAP kinase and
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studies such as the NCS, consider h
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the beginning of the academic caree
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trophil infiltration, a significant
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tactic response and optokinetic res
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usually high spontaneous PIG-A MFs.
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699 PROMUTAGEN ACTIVATION AND P450
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oxodG in bone marrow, spleen, kidne
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718 GENOTOXICITY OF ORGANIC EXTRACT
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ment were 18.0 and 4.5 nM for BEAS-
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strongest activation of nuclear fac
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746 MACROPHAGE INHIBITORY CYTOKINE-
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screening of cell migration. High-c
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dase inhibition). In contrast, inhi
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mice; the decrease was more pronoun
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Markers of oxidative damage reached
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793 PULMONARY RESPONSE, OXIDATIVE S
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cilitate clinical and industrial ap
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sion of p-p38, p-p53, p21 and cycli
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821 KIDNEY INJURY MOLECULE-2 GENE D
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commercial applications, and have b
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developmental effects. The residual
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strand breaks in an in vitro model
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etate (immunotoxicity). 2,4-D was t
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867 MELATONIN AMELIORATES CYCLOPHOS
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pharmacokinetic (PBPK) models. Ten
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of radiolabeled Mn (carrier-free 54
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893 UNVEILING ASSOCIATIONS BETWEEN
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using area under the concentration
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912 COMMERCIAL FISH DIETS INDUCE VI
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922 A STUDY OF PHOTOTOXICITY FOLLOW
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from 0.1 to 2.9 g/L (saturated vapo
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vasive method for assessing several
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950 ARSENITE DOWN-REGULATES THE CAR
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inflammatory signaling is altered b
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treated wood. Seventy-five of 132 w
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ergy homeostasis and raising levels
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treated with mercury and then with
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997 IN VIVO CORRELATES OF THE MANGA
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of the panel. The panel was compris
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sponse, location, and severity scor
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tant source of n-3 fatty acids such
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old for serious, irreversible or es
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1045 SUBCHRONIC TOXICITY EVALUATION
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sites collected 3 days after inject
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1063 MOLECULAR MECHANISM OF OLANZAP
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esponses, and can be available for
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hence more physiologically relevant
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thesized apoproteins, so we tested
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vate CYP3A5 but could be released b
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1108 STYRENE-INDUCED TOXIC EFFECTS
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1118 THE PRESENCE OF DIETHYL FUMARA
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zebrafish cells were first exposed
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utene-1,4-dial, which has been show
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groups, largely due to lower non-HD
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Exposure to gluten in individuals w
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contrast to VGSC activators such as
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1175 70% HYDROFLUORIC ACID (HF) CUT
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axis. An increase in hyaline drople
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1194 esiRNA AND siRNA HIGH-THROUGHP
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1203 ARYL HYDROCARBON RECEPTOR-DNA
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permeability (calcein), mitochondri
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olytic caspase activation, caspase-
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teristics of a human T-type voltage
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80% of the activity from the yellow
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1251 DEVELOPMENTAL EXPOSURE TO DELT
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1260 MANEB ENHANCES MPP + -INDUCED
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demonstrated by knockdown of beclin
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1278 PINK1 AND MITOCHONDRIAL DYNAMI
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treatment for number of live worms.
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1297 INVESTIGATION OF THE NEUROTOXI
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1306 DEVELOPMENT OF AN IN VITRO ASS
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1315 EVALUATION OF 3- AND 1-METHYLH
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prior to kainic acid-induced seizur
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1334 AFFECT OF GENETIC VARIATION ON
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1346 THE OTHER WORLD OF THE TRANSCR
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strate, leading to excess microvesi
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1368 OVERVIEW OF THERAPEUTIC VACCIN
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to a bovine adrenal cortical epithe
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DOTC had no effects. These results
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1398 PULMONARY TOXICITY OF CERIUM D
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PPARα, LXR, ER, AR and AhR activit
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1417 MECHANISM OF GENDER-DIVERGENT
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els, they disrupt homeostatic contr
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were to characterize exposure of th
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1448 ADVERSE OUTCOME PATHWAYS AND E
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the level in urine was 25% of the m
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1;akt-2 double mutant and pdk-1 mut
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jury effects when compared to contr
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tude than equivalent oral doses. Af
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cells; and increased iNOS and MCP-1
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B6.Cg-Kit W-sh mice. These findings
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1511 STATIN-TREATMENT EFFECTIVELY R
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1520 EFFECT OF INHALATION EXPOSURE
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numbers of IFN-γ producing cells w
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These data indicate that TCDD treat
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esponse to allogenic cells, where P
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larly suppressed LPS-induced TNF-α
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1565 INFLUENCE OF EXPOSURE ROUTE AN
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veloping animals to adverse effects
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the observed CL values were 0.07 L/
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which is most relevant for potentia
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tive impairment, suggesting that ox
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1611 AN IN VITRO METABOLOMICS APPRO
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Expression of the β6 integrin (Itg
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ats (10 μg/kg TCDD). Here we repor
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at liver slices and how the metabon
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with gender differences in AUC and
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oxetine (30 mg/kg reduced to 15 mg/
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eleased from necrotic cells where i
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plants, they are present in surface
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ferentiation (quantitative in-cell
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control for macrophage activation).
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to 0.5μg/ml. Minimal inhibitory co
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lines tested. Given that dex and AT
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tion in the absence of an immune re
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treated rats had lower calcium load
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1740 PROFILING COMPOUNDS WITH CARDI
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dothelial cells confirmed caveolin-
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1758 GINKGO BILOBA EXTRACT INDUCES
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arin-induced suppression of MDR1 ex
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1780 ANTIANDROGENIC AND ANTIPROLIFE
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included in the evaluation, most of
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1799 GUIDANCE ON THE APPLICATION OF
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However, substances with EC3 values
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1816 CD-INDUCED EGFR TRANSACTIVATIO
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1826 KERATIN 7 EXPRESSION IN INDEPE
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centa were collected at the time of
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1845 SYSTEMATIC SCREENING OF YEAST
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underlying the development of co-mo
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eing measured in experimental roden
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ufactured in the US for more than 3
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nine (N7-THB-Gua) and N7-hydroxyphe
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1892 EFFECT OF LAMBDA-CYHALOTHRIN O
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22 in Phase I. Antimicrobial pestic
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kinetic and dynamic differences, an
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iphenyl, saccharin and melamine was
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1930 DEVELOPMENT OF A RELATIVE SOUR
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1939 MODE OF ACTION FOR THE CANCER
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human was about 1.5-fold lower (60
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Disruption of BDEC integrity in alp
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1968 A HUMAN HEPG2 LUCIFERASE ASSAY
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in the offspring during tumor devel
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een developed to investigate perfus
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to 131.73 μg/mL for KLH-specific I
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cell lines (ATCC) were cultured in
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flammation and xenobiotic metabolis
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vide many contributions: with its g
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to-metal joint replacement. For exa
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to be addressed or negotiated. Deci
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especially with anti-TNF therapies.
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genic line Tg(are:eGFP) was created
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2074 COMPUTATIONAL ASSESSMENT OF TH
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2084 INHIBITION OF 11β-HSD1 DECREA
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(T) and express several enzymes inv
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2101 GENISTEIN MODULATION OF BLOOD
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males per group were dosed orally o
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ate the feasibility of assessing re
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changed. Hepatic protein carbonyl l
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sought to examine alterations in he
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2147 A SINGLE AMINO ACID CONTROLS T
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Gstm7) was independent of both CAR
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ility of tungsten trioxide (WO3), t
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ured by real-time PCR array and rel
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glucose, and creatinine levels, and
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Several studies have reported suppr
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exposure between TCDD-exposed labor
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Furthermore, with increasing number
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Society of Toxicology 2010 Author I
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Society of Toxicology 2010 Abstract
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49 th Annual Meeting and ToxExpo A
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The Official Journal of the Society
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