Nikon A1r Laser Scanning Confocal & 2-Photon Microscope

iii) “Do Not Stitch” simply places tiles next to each other without processing them
***This type of acquisition WILL NOT give individual images for
each tile***
e) Channels – lambda tab
i) This allows you to switch between optical configurations that require a change
in dichroic, light source, IR laser wavelength, or detectors
f) Order of Experiment
i) The drop down menu is written multiplicatively, the experiment will be
executed in the order of options RIGHT to LEFT
25) Image Window
After the acquisition has finished there are several choices for viewing your data
a) Split channel will show each channel as an individual frame
b) Volume view gives an interactive 3D rendering of your data
c) Tile view displays your Zs as a series of thumbnails
d) Maximum intensity projection takes the brightest pixel at each pixel location in
your Z-stack and displays a single image
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