haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
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Haemostasis (page 2 of 6)<br />
Test Specimen type Key Factors<br />
affecting tests<br />
Anti-Xa assay<br />
For monitoring<br />
low molecular<br />
weight heparin -<br />
LMWH) therapy.<br />
β2 Glycoprotein<br />
1 Antibodies<br />
(B2GPI)<br />
Bleeding screen<br />
1) von<br />
Willebrand<br />
Factor<br />
Antigen<br />
2) von<br />
Willebrand<br />
Factor Activity<br />
1 x 4.5ml Sodium<br />
Citrated sample<br />
1 x 5ml SST<br />
sample<br />
7 x 4.5ml Sodium<br />
Citrated samples<br />
Recommendation:<br />
also send an<br />
EDTA sample for<br />
a FBC.<br />
Delayed receipt<br />
of sample<br />
Heparin affects<br />
FVIII result.<br />
Delayed receipt<br />
of sample.<br />
Notes<br />
Samples should<br />
be collected 4<br />
hours post an<br />
injection of Low<br />
Molecular Weight<br />
Heparin.<br />
A single SST<br />
sample is<br />
sufficient for<br />
ACLA & B2GPI<br />
Antibody testing.<br />
Reference<br />
Range<br />
Therapeutic<br />
range: 0.5 – 1.0<br />
IU/ml.<br />
Individual<br />
patient’s target<br />
range is a clinical<br />
decision.<br />
IgG: < 10 U/ml<br />
IgM: < 6 U/ml<br />
FVIII: 0.50 –<br />
2.00 IU/ml<br />
VWFAg: 0.50 –<br />
1.60 IU/ml<br />
VWFAct: 0.63 –<br />
2.00 IU/ml<br />
Out of hours<br />
service<br />
By agreement<br />
with the<br />
Haematology<br />
Consultant on call<br />
and subject to<br />
staff availability.<br />
No out of hours<br />
service<br />
Factor VIII only,<br />
by agreement<br />
with the<br />
Haematology<br />
Consultant on call<br />
and subject to<br />
staff availability.<br />
Time Limit for<br />
requesting<br />
additional tests<br />
from time of<br />
venesection<br />
Contact the<br />
Laboratory<br />
Contact the<br />
Laboratory<br />
Contact the<br />
Laboratory<br />
Turnaround time<br />
from receipt of<br />
sample (in<br />
working days for<br />
non urgent<br />
requests)<br />
Urgent:<br />
12 hrs<br />
Non-urgent:<br />
5 working days<br />
3 working day<br />
3 working days<br />
3) Factor VIII<br />
assays<br />
4) Platelet<br />
Aggregation<br />
Haemostasis (page 3 of 6)<br />
HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 21 of 38<br />
Authors: Karen Orfinada (Senior Biomedical Scientist)<br />
Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015
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