haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
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Vacutainer Guide<br />
Becton Dickinson<br />
Vacutainer <br />
Note : underfilled samples<br />
are unsuitable for testing.<br />
Ordering<br />
Information<br />
EDTA<br />
4.5 ml<br />
Sodium citrate (citrated<br />
tube)<br />
4.5 ml<br />
SST<br />
5ml<br />
Lithium Heparin<br />
4.5 ml<br />
Sodium citrate (citrate)<br />
1.0 ml<br />
Tests / Screens<br />
FBC, ESR, Reticulocyte count, IPF, Blood film (note: ESRs cannot be performed on 7ml EDTA tubes),<br />
Sickle screen, Malaria Antigen testing<br />
Glandular Fever testing (note, Glandular Fever testing can be performed on an SST sample),<br />
Lymphocyte subsets (including CD4, CD8 counts), Hereditary Spherocytosis Dye binding assay,<br />
Plasma Viscosity, PCR in Haemostasis<br />
Blood Transfusion<br />
*Group & Save (including antibody screen), *Crossmatch, Kleihauer, DAT<br />
*Sample identification for Group & Screen or Crossmatch MUST be hand written with 4 correct points of ID.<br />
Clotting screen / Fibrinogen / D-Dimers. These tubes must be filled exactly to the 4.5ml mark on the side of<br />
the tube: underfilled & overfilled samples are unsuitable for testing.<br />
For Paediatric Clotting Screens, see below.<br />
Citrated Platelet Count (alert Coagulation laboratory staff that the sample is en route: it must not be<br />
centrifuged).<br />
Specialised Haemostasis tests must be authorised by a Haematology/Haemostasis SpR/Consultant<br />
See the Haemostasis section below for the samples required for specialised Haemostasis tests.<br />
Cardiolipin antibodies (Antiphospholipid antibodies)<br />
β 2 GPI antibodies<br />
Haemoglobinopathy screen, G6PD assay, Pyruvate Kinase assay, Red Cell Osmotic Fragility, Globin Chain<br />
Biosynthesis, Oxygen Dissociation curve<br />
Cytogenetic chromosomal analysis and FISH.<br />
Paediatric Clotting Screen, Fibrinogen, D-Dimers (1ml tubes).<br />
Contact the Haemostasis laboratory for other Haemostasis tests for paediatric patients.<br />
These tubes must be filled exactly to the 1ml mark on the side of the tube: underfilled & overfilled samples<br />
are unsuitable for testing.<br />
These tubes are issued from the Haemostasis Laboratory to the Neonatal Unit, Paediatric A&E and<br />
Phlebotomy Outpatient Departments and can be issued to other locations on request: contact x78549 or<br />
x78545. Monday – Friday 09.00 – 17.15<br />
HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 7 of 38<br />
Authors: Karen Orfinada (Senior Biomedical Scientist)<br />
Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015
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