haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
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Leukaemia Cytogenetics (page 1 of 5)<br />
2 nd Floor, 60 Whitfield St <strong>London</strong> W1T 4EU Hours: 09.00 – 17.00 Monday - Friday Tel: 0203 447 9603 or x 79603<br />
• All samples for Leukaemia Cytogenetics are sent from the Flow Cytometry Laboratory to referral Laboratories.<br />
Test<br />
Specimen<br />
type<br />
Key Factors<br />
affecting tests<br />
Notes<br />
Out of<br />
hours<br />
service<br />
Time Limit<br />
for<br />
requesting<br />
additional<br />
tests from<br />
time of<br />
venesection<br />
Turnaround<br />
time from<br />
receipt of<br />
sample (in<br />
working<br />
days)<br />
Cytogenetic (chromosomal analysis)<br />
-Testing for suspected haematological<br />
disease disorders<br />
(at presentation, post therapy or bone<br />
marrow transplant).<br />
Aplastics/ general anaemia<br />
Acute Myeloid Leukaemia –<br />
M1,M2,M3,M4,M4Eo,M5<br />
A.L.L<br />
Burkitt’s Lymphoma<br />
CLL<br />
CML<br />
Diffuse Large B cell Lymphoma<br />
ET<br />
Follicular Lymphoma<br />
Mantle cell lymphoma<br />
MDS (CMML/RA/RARS/RAEB)<br />
Myelofibrosis<br />
Multiple Myeloma<br />
MPD (ET/Polycythaemia)<br />
NHL<br />
Pancytopenia<br />
Plasma cell Leukaemia<br />
PRV<br />
Thrombocytopenia/Neutropenia<br />
Bone Marrow<br />
Peripheral<br />
Blood:<br />
5-10ml<br />
Samples<br />
should be in<br />
Lithium<br />
Heparin or<br />
Cytopots<br />
(RPMI 1640 +<br />
Heparin<br />
medium).<br />
Except for<br />
CD138+<br />
isolated<br />
plasma cells<br />
for FISH on<br />
multiple<br />
myeloma<br />
cases.<br />
Poor sample<br />
quality (clotted<br />
or more than a<br />
day old)<br />
Sample<br />
transported and<br />
or stored above<br />
or below room<br />
temperature<br />
Sample<br />
Contaminated<br />
by bacteria<br />
Sample taken<br />
into wrong<br />
transport<br />
medium<br />
Sent to: Great Ormond Street<br />
Hospital, Paediatric<br />
Malignancy Unit (PMU)<br />
Samples must be received<br />
before 2pm (Monday-Friday)<br />
for samples to get to PMU on<br />
the same day.<br />
Analysis will be carried as<br />
appropriate or in consultation<br />
with the referring Consultant<br />
or Registrar.<br />
The samples may be left<br />
overnight at room temperature<br />
and delivered to laboratory<br />
immediately the following<br />
morning.<br />
No<br />
service<br />
provided<br />
N/A<br />
Urgent<br />
referrals:<br />
10 working<br />
days<br />
Routine<br />
requests:<br />
15 working<br />
days<br />
HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 34 of 38<br />
Authors: Karen Orfinada (Senior Biomedical Scientist)<br />
Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015
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