haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
haematology users' handbook - University College London Hospitals
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Leukaemia Cytogenetics (page 2 of 5)<br />
Test Specimen type Key Factors<br />
affecting tests<br />
Notes<br />
Out of<br />
hours<br />
service<br />
Time Limit<br />
for<br />
requesting<br />
additional<br />
tests from<br />
time of<br />
venesection<br />
Turnaround<br />
time from<br />
receipt of<br />
sample (in<br />
working<br />
days)<br />
Cytogenetic FISH (Fluorescent<br />
Insitu Hybridisation) Analysis<br />
-Testing for suspected<br />
haematological disease disorders<br />
(at presentation, post therapy or<br />
bone marrow transplant).<br />
Aplastic anaemia<br />
AML-M1,M2,M3,M4,M4Eo,M5<br />
A.L.L<br />
Burkitt’s Lymphoma<br />
CLL<br />
CML<br />
Diffuse Large B cell Lymphoma<br />
ET<br />
Follicular Lymphoma<br />
Hypereosinophilic Syndrome<br />
Mantle cell lymphoma<br />
MDS<br />
Multiple Myeloma<br />
MPD (ET/Polycythaemia)<br />
NHL<br />
Plasma cell Leukaemia<br />
PRV<br />
As above<br />
Bone Marrow or<br />
Peripheral Blood<br />
in EDTA for FISH<br />
only.<br />
CD138+ isolated<br />
plasma cells for<br />
FISH on multiple<br />
myeloma cases is<br />
preferable<br />
however bone<br />
marrow samples<br />
may also be<br />
adequate.<br />
Poor sample<br />
quality (clotted<br />
or more than a<br />
day old)<br />
Sample<br />
transported<br />
and or stored<br />
above or below<br />
room<br />
temperature<br />
Sample<br />
Contaminated<br />
by bacteria<br />
Sample taken<br />
into wrong<br />
transport<br />
medium<br />
Sent to: Great Ormond Street<br />
Hospital, Paediatric Malignancy<br />
Unit (PMU)<br />
Samples must be received before<br />
2pm (Monday-Friday) for samples<br />
to get to PMU on the same day.<br />
Analysis will be carried as<br />
appropriate or in consultation with<br />
the referring Consultant or<br />
Registrar.<br />
The samples may be left<br />
overnight at room temperature<br />
and delivered to laboratory<br />
immediately the following<br />
morning.<br />
No<br />
service<br />
provided<br />
N/A<br />
Rapid FISH:<br />
3 working<br />
days<br />
Urgent<br />
referrals:<br />
10 working<br />
days<br />
Routine<br />
requests:<br />
15 working<br />
days<br />
HAEM-PD-UserHbook V8 Haematology and Blood Transfusion User Handbook Page 35 of 38<br />
Authors: Karen Orfinada (Senior Biomedical Scientist)<br />
Sally McDonald (Senior Biomedical Scientist) Authorised by: Dave Barnett Review Date: August 2015
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