Introduction to bioinformatics - National Genetics Reference ...

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polyA_site 6132 ORIGIN /gene="CFTR" /gene_synonym="ABC35; ABCC7; CF; CFTR/MRP; dJ760C5.1; MRP7; TNR-CFTR" The Sequence 1 aattggaagc aaatgacatc acagcaggtc agagaaaaag ggttgagcgg caggcaccca 61 gagtagtagg tctttggcat taggagcttg agcccagacg gccctagcag ggaccccagc 121 gcccgagaga ccatgcagag gtcgcctctg gaaaaggcca gcgttgtctc caaacttttt 181 ttcagctgga ccagaccaat tttgaggaaa ggatacagac agcgcctgga attgtcagac 241 atataccaaa tcccttctgt tgattctgct gacaatctat ctgaaaaatt ggaaagagaa 301 tgggatagag agctggcttc aaagaaaaat cctaaactca ttaatgccct tcggcgatgt 361 tttttctgga gatttatgtt ctatggaatc tttttatatt taggggaagt caccaaagca 421 gtacagcctc tcttactggg aagaatcata gcttcctatg acccggataa caaggaggaa 481 cgctctatcg cgatttatct aggcataggc ttatgccttc tctttattgt gaggacactg
Issue 7: sequence formats – how many?! • Many different sequence formats • Different pieces of software use different ones, so check yours is in the correct format • They are ASCII Text and use the IUPAC standard one-letter code. • Most sequence formats include at least one ID name, placed usually somewhere <strong>to</strong>wards the <strong>to</strong>p of the sequence format • You can convert between different formats using the program seqret (http://embossgui.sourceforge.net/demo/seqret.html)
Page 1 and 2: Shedding some light on Bioinformati
Page 3 and 4: What is Bioinformatics? “Bioinfor
Page 5: GENE EXPRESSION ANALYSIS COMPARATIV
Page 8 and 9: Growth of Sequences and Annotations
Page 10 and 11: Computers - they are your workbench
Page 12 and 13: Computer Software On a computer the
Page 14 and 15: Major Bioinformatics Databases •
Page 16 and 17: Nucleic Acid Sequence Databases •
Page 18 and 19: Issue 1: Why so many IDs? Biology!
Page 20 and 21: Issue 3: Data redundancy COMMENT RE
Page 22 and 23: CDS 133..4575 Database entries usua
Page 24 and 25: FEATURES Location/Qualifiers source
Page 26 and 27: NCBI Map of links between Entrez no
Page 28 and 29: An accession number and identifer N
Page 30: Issue 5 cntd. • Most databases st
Page 34: Issue 6 cntd: : Standard vocabulary
Page 39 and 40: Multiple FASTA sequences in one fil
Page 41: Sequence Retrieval System (SRS) •
Page 44 and 45: Issue 8: Keeping up-to to-date with
Page 46 and 47: Issue 10: Data Security and Clinica
Page 48: We all are bioinformaticians! “Bi

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