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SaltRx 1 • SaltRx 2 • SaltRx HT application n Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules features n Developed at Hampton Research n Salt versus pH matrix crystallization screen n Samples pH 4 - 9 n 22 unique salts versus salt concentration and pH n Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods n Preformulated, ready to screen n All salts and buffers in screen readily available as Optimize reagents for reproducing and optimizing crystals n Tube or Deep Well block format description SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD 1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt based reagents/conditions from screens such as Index, Crystal Screen, and Grid Screen produce crystals and when further screening for additional salt conditions or optimization is desired. SaltRx is designed to be used as a 96 reagent screen. SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 2 contains 48 unique reagents, 10 ml each. SaltRx HT contains 1 ml of each reagent from SaltRx 1 and SaltRx 2 in a single Deep Well block format. success story Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. References 1. Gilliland, G.L., Tung, M., Blakeslee, D.M. and Ladner, J. 1994. The Biological Macromolecule Crystallization Database, Version 3.0: New Features, Data, and the NASA Archive for Protein Crystal Growth Data. Acta Crystallogr. D50 408-413. 2. Crystallization and preliminary crystallographic analysis of molybdenumcofactor biosynthesis protein C from Thermus thermophilus. S. P. Kanaujia, C. V. Ranjani, J. Jeyakanthan, S. Baba, L. Chen, Z.-J. Liu, B.-C. Wang, M. Nishida, A. Ebihara, A. Shinkai, S. Kuramitsu, Y. Shiro, K. Sekar and S. Yokoyama. Acta Cryst. (2007). F63, 27-29. Order Information SaltRx 1 and SaltRx 2 kits each contain 48 unique reagents. To order individual reagents, use Custom Shop catalog number listed below. Refer to page 36 for further details. Cat. No. Name Description Price HR2-107 SaltRx 1 10 ml, tube format $285.00 HR2-109 SaltRx 2 10 ml, tube format $285.00 HR2-136 SaltRx HT 1 ml, Deep Well block format $185.00 HR2-907-** SaltRx 1 Custom Shop 185 ml $138.00 HR2-909-** SaltRx 2 Custom Shop 185 ml $138.00 ** = reagent number 1-48 screens Crystals grown using SaltRx, reagent 62. They were grown using the Cyberlab C240 robot and the Neuro Probe plate. These were grown just in time to freeze and take to the synchrotron (no time for optimizations). MAD data were collected on these crystals and the structure has been solved to 2.3Å resolution. Courtesy of Annie Hassell. GlaxoSmithKline 16 Hampton Research • Phone: 800-452-3899 or 949-425-1321 • Fax: 949-425-1611 • www.hamptonresearch.com • Customer Service: info@hrmail.com • Technical Support: tech@hrmail.com
saltrx 1 formulation A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 B1 B2 B3 B4 B5 B6 B7 B8 B9 B10 B11 B12 C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 C12 D1 D2 D3 D4 D5 D6 D7 D8 D9 D10 D11 D12 1. 1.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 2. 2.8 M Sodium acetate trihydrate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 3. 1.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6 4. 1.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0 5. 1.5 M Ammonium chloride, 0.1 M Tris pH 8.5 6. 3.5 M Ammonium chloride, 0.1 M Sodium acetate trihydrate pH 4.6 7. 3.5 M Ammonium chloride, 0.1 M BIS-TRIS propane pH 7.0 8. 3.5 M Ammonium chloride, 0.1 M Tris pH 8.5 9. 2.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6 10. 2.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0 11. 2.2 M Sodium chloride, 0.1 M Tris pH 8.5 12. 3.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6 13. 3.2 M Sodium chloride, 0.1 M BIS-TRIS propane pH 7.0 14. 3.2 M Sodium chloride, 0.1 M Tris pH 8.5 15. 1.0 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6 16. 1.8 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6 17. 1.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 18. 2.0 M Ammonium citrate tribasic pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 19. 0.7 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0 20. 0.7 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5 21. 1.2 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane pH 7.0 22. 1.2 M Sodium citrate tribasic dihydrate, 0.1 M Tris pH 8.5 23. 0.4 M Magnesium formate dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6 24. 0.4 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0 25. 0.4 M Magnesium formate dihydrate, 0.1 M Tris pH 8.5 26. 0.7 M Magnesium formate dihydrate, 0.1 M BIS-TRIS propane pH 7.0 27. 2.0 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6 28. 2.0 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0 29. 2.0 M Sodium formate, 0.1 M Tris pH 8.5 30. 3.5 M Sodium formate, 0.1 M Sodium acetate trihydrate pH 4.6 31. 3.5 M Sodium formate, 0.1 M BIS-TRIS propane pH 7.0 32. 3.5 M Sodium formate, 0.1 M Tris pH 8.5 33. 1.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 34. 2.2 M DL-Malic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 35. 1.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 36. 2.4 M Sodium malonate pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 37. 2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6 38. 2.5 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0 39. 2.5 M Ammonium nitrate, 0.1 M Tris pH 8.5 40. 6.0 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6 41. 6.0 M Ammonium nitrate, 0.1 M BIS-TRIS propane pH 7.0 42. 6.0 M Ammonium nitrate, 0.1 M Tris pH 8.5 43. 1.5 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6 44. 1.5 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0 45. 1.5 M Sodium nitrate, 0.1 M Tris pH 8.5 46. 4.0 M Sodium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6 47. 4.0 M Sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0 48. 4.0 M Sodium nitrate, 0.1 M Tris pH 8.5 BIS-TRIS propane Sodium acetate trihydrate Tris buffer organic salt Ammonium citrate dibasic Ammonium citrate tribasic Magnesium formate dihydrate Sodium acetate Sodium citrate tribasic dihydrate Sodium formate Sodium malonate DL-Malic acid Range from 4.6 to 8.5 pH saltrx 1 factors salt Ammonium chloride Sodium chloride Ammonium nitrate Sodium nitrate saltrx 2 formulation E1 E2 E3 E4 E5 E6 E7 E8 E9 E10 E11 E12 F1 F2 F3 F4 F5 F6 F7 F8 F9 F10 F11 F12 G1 G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 H1 H2 H3 H4 H5 H6 H7 H8 H9 H10 H11 H12 1. 1.0 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6 2. 1.8 M Ammonium phosphate monobasic, 0.1 M Sodium acetate trihydrate pH 4.6 3. 1.5 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5 4. 2.4 M Ammonium phosphate dibasic, 0.1 M Tris pH 8.5 5. 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0 6. 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9 7. 1.0 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2 8. 1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 5.0 9. 1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 6.9 10. 1.8 M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic / pH 8.2 11. 0.5 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 12. 1.0 M Succinic acid pH 7.0, 0.1 M BIS-TRIS propane pH 7.0 13. 1.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6 14. 1.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0 15. 1.5 M Ammonium sulfate, 0.1 M Tris pH 8.5 16. 2.5 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6 17. 2.5 M Ammonium sulfate, 0.1 M BIS-TRIS propane pH 7.0 18. 2.5 M Ammonium sulfate, 0.1 M Tris pH 8.5 19. 0.8 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6 20. 0.8 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0 21. 0.8 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5e 22. 1.5 M Lithium sulfate monohydrate, 0.1 M Sodium acetate trihydrate pH 4.6 23. 1.5 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS propane pH 7.0 24. 1.5 M Lithium sulfate monohydrate, 0.1 M Tris pH 8.5 25. 1.0 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6 26. 1.0 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0 27. 1.0 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5 28. 1.8 M Magnesium sulfate hydrate, 0.1 M Sodium acetate trihydrate pH 4.6 29. 1.8 M Magnesium sulfate hydrate, 0.1 M BIS-TRIS propane pH 7.0 30. 1.8 M Magnesium sulfate hydrate, 0.1 M Tris pH 8.5 31. 0.7 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6 32. 0.7 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0 33. 0.7 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5 34. 1.1 M Ammonium tartrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6 35. 1.3 M Ammonium tartrate dibasic, 0.1 M BIS-TRIS propane pH 7.0 36. 1.4 M Ammonium tartrate dibasic, 0.1 M Tris pH 8.5 37. 0.6 M Potassium sodium tartrate tetrahydrate 0.1 M BIS-TRIS propane pH 7.0 38. 1.2 M Potassium sodium tartrate tetrahydrate 0.1 M BIS-TRIS propane pH 7.0 39. 0.6 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5 40. 1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris pH 8.5 41. 0.5 M Potassium thiocyanate, 0.1 M Sodium acetate trihydrate pH 4.6 42. 0.5 M Potassium thiocyanate, 0.1 M BIS-TRIS propane pH 7.0 43. 0.5 M Potassium thiocyanate, 0.1 M Tris pH 8.5 44. 4.0 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6 45. 0.1 M BIS-TRIS propane pH 7.0, 4.0 M Ammonium Acetate 46. 4.0 M Ammonium acetate, 0.1 M Tris pH 8.5 47. 35% v/v Tacsimate , 0.1 M BIS-TRIS propane pH 7.0 48. 60% v/v Tacsimate , 0.1 M BIS-TRIS propane pH 7.0 BIS-TRIS propane Sodium acetate trihydrate Tris buffer Range from 4.6 to 8.5 pH Tacsimate TM organic salt Ammonium acetate Ammonium tartrate dibasic K/Na Tartrate Succinic acid saltrx 2 factors salt Ammonium phosphate mono. Ammonium phosphate dibasic Ammonium sulfate Lithium sulfate monohydrate Magnesium sulfate hydrate Na/K Phosphate Potassium thiocyanate screens 17
Page 1 and 2: C R Y S T A L L I Z A T I O N volum
Page 3 and 4: table of contents PAGES 2 - 33 scre
Page 5 and 6: table of contents PAGES 5 pct pre-c
Page 7 and 8: pct Pre-Crystallization Test applic
Page 9 and 10: index f o r m u l a t i o n A1 A2 A
Page 11 and 12: crystal screen formulation A1 A2 A3
Page 13 and 14: PEGRx 1 formulation A1 A2 A3 A4 A5
Page 15 and 16: PEg/ion screen formulation A1 A2 A3
Page 17: Order Information Each Grid Screen
Page 21 and 22: MEMBFAC formulation A1 A2 A3 A4 A5
Page 23 and 24: natrix formulation 1. 0.01 M Magnes
Page 25 and 26: crystal screen cryo formulation 1.
Page 27 and 28: Low Ionic Strength Screen applicati
Page 29 and 30: organic salts organic acids macromo
Page 31 and 32: Detergent Screen • detergent scre
Page 33 and 34: Heavy Atom Screen Pt (Platinum) 1.
Page 35 and 36: Crystal Image. Allan D’Arcy, Swit
Page 37 and 38: table of contents PAGES 36 - 37 cus
Page 39 and 40: StockOptions ADA - 185 ml $208.00 C
Page 41 and 42: table of contents Page Pre-Crystall
Page 43 and 44: Polymers Polyethylene glycol 300 HR
Page 45 and 46: Salts Ammonium acetate HR2-565 1.0
Page 47 and 48: Salts Lithium acetate dihydrate HR2
Page 49 and 50: Salts Potassium phosphate monobasic
Page 51 and 52: Salts Sodium tartrate dibasic dihyd
Page 53 and 54: Buffers Imidazole Buffer (Useful pH
Page 55 and 56: Optimize Silica - Reducing Hydrogel
Page 57 and 58: Silica Optimize Hydrogel - Oils Flu
Page 59 and 60: Optimize - Silica Hydrogel kit appl
Page 61 and 62: Nuclear receptor crystal. Gilbert B
Page 63 and 64: table of contents PAGES 62 stockopt
Page 65 and 66: StockOptions p H application n Cry
Page 67 and 68: stockoptions sodium citrate buffer
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stockoptions tris HO NH 2 OH pH 7.0
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"Butterfly"-crystal of hydroxynitri
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table of contents PAGES 72 - 77 24
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n Hanging Drop Crystallization n Si
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VDXm Plate application n Hanging d
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VDX Plate (Modular) applications n
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VDX48 Plate with sealant applicatio
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CrystalEX (Corning) application n S
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CrystalQuick 96 Well Sitting Drop P
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M R C 2 W e l l C r y s t a l l i z
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M R C U n d e r O i l 9 6 W e l l C
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Microbatch 96 Well Plate applicatio
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Cover Slides & Related Tools Silico
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Plain Glass Cover Slides applicatio
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Duster - Canned Air application n D
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9 well glass plate 9 W e l l G l a
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Sealing Films, Tapes, Mats & Covers
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AlumaSeal II sealing film and appli
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Applicator for Dialysis Buttons app
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Protein crystals. Kasumi Kobayashi,
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table of contents PAGES 108 micro-t
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Micro-Tools ii application n Precis
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Small Forcep features n Length - 14
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Locking Forcep features n Length -
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Seeding tool application n Streak S
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table of contents PAGES 118 - 120 c
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CrystalCap Copper Magnetic ALS HT a
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CrystalCap Magnetic systems Crystal
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CrystalCap systems CrystalCap - C o
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Adjustable Mounted CryoLoop applica
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CryoLoops - 10 micron application n
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CryoTong - L o n g application n Cr
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Vial Clamp - Straight application n
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Canes, Sleeves & Coders CryoCane ap
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Foam Dewar application n Cryocrysta
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table of contents PAGES 138 - 140 c
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special glass 10 (SODA LIME GLASS)
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wicks paper wicks applications n Re
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Waxes & Clay beeswax applications n
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Magnetic Mounts & Supports Adjustab
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table of contents PAGES 148 xyz hea
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XYZ Heated Goniometer Head supplies
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XYZ Goniometer Head supplies Storag
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Magnetic Base Support Magnetic Base
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table of contents PAGES 156 xenon c
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C a s e S t u d y : X e n o n D e r
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table of contents PAGES 160 p ens 1
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tough-tags® on a roll Tough-Tags®
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Laser Cryo-Babies® and Cryo-Tags®
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table of contents PAGES 166 macromo
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protein Crystallization Strategies
Page 171 and 172:
Crystallography Made Crystal Clear
Page 173 and 174:
Macromolecular Crystallization Meth
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Protein Crystallization n Editor: T
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table of contents PAGES 176 glucose
Page 179 and 180:
lysozyme application n Crystallizat
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Lipase B application n Protein stan
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table of contents PAGES 182 - 183 p
Page 185 and 186:
Solutions for Crystal Growth Page 2
Page 187 and 188:
Oil Crystallization Drop Solutions
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Solutions for Crystal Growth sittin
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Solutions for Crystal Growth dialys
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Solutions for Crystal Growth v i e
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Solutions for Crystal Growth salt o
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1 1.5 2 2.5 3 4 Solutions for Cryst
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Solutions for Crystal Growth protei
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Solutions for Crystal Growth What c
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Solutions for Crystal Growth h o w
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Solutions for Crystal Growth micros
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Solutions for Crystal Growth p r o
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Solutions for Crystal Growth temper
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Crystal image. Anastasia Tziridis,
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tips from ramc Tips from Recent Adv
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c r y s t a l l i z a t i o n tips
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crystallization tips Solutions for
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pH BUFFER 1 2 3 4 5 6 7 8 9 10 11 1
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Reagent Formula MW Temp (°C) g/100
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index index A ADA..................
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index index HR1-002................
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index index HR4-CONTINUED HR4-116..
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general information orders By phone
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