Download - Hampton Research

Optimize - Silica Hydrogel kit
application
n Quick & easy kit format for crystallization in
gels
features
n Proprietary Silica Hydrogel formulation with
neutral pH
n Gel matrix can reduce nucleation &
sedimentation
description
Gels are a very efficient media for growing macromolecular crystals.
1-5 Silica gels in particular have the advantage in that they are
stable, usable over a wide range of temperatures (0-60°C), and are
compatible with a wide variety of precipitants and additives used
for crystal growth.
Gels can reduce nucleation and sedimentation, provide added stability,
and allow crystals to grow larger. The porous network minimizes
natural convection. Crystals are suspended in the gel network
so that they do not form sediments and can grow free from
strain exerted by the container or other crystals. Heterogeneous
and secondary nucleation are reduced in the presence of a Silica Hydrogel. 3 The Silica Hydrogel can
be used for liquid-gel, liquid-gel-liquid, vapor diffusion, as well as dialysis crystallization methodologies.
The gel is compatible with a wide range of salts, polymers, organic solvents, and buffers used for
macromolecular crystallization in a pH range from 3 to 10.
Each Silica Hydrogel kit contains 12 tubes of Sodium silicate solution and 12 tubes of Acetic acid solution,
500 µl each. All solutions are sterile filtered and formulated using ultra-pure water. Crystallization
accessories are sold separately.
References
1. Robert, M.C. & Lefaucheux, F., J. Crystal Growth (1988) 90, 358.
2. Provost, K. & Robert, M.C., J. Crystal Growth (1991) 110, 258.
3. M.C. Robert, K. Provost, & F. Lefaucheux, Crystallization of Nucleic Acids and Protein, A Practical Approach, Oxford Univ Press (1992) 127-143.
4. McPherson A., Methods in Enzymology (1985) 114, 112.
5. Cudney, B., Patel, S., McPherson, A., Acta Cryst. (1994) D50, 479-483.
Order Information
Cat. No. Name Description Price
application
n Crystallization in agarose gel
features
n Gel matrix can reduce nucleation and sedimentation
n Crystallization grade
n Low melting agarose
HR2-310 Silica Hydrogel 500 µl, 24 tubes $95.00
description
Low melting (LM) agaroses are the result of a derivatization process
by organic synthesis. The main properties of these agaroses are
their low melting and gelling temperatures when compared with
standard agaroses. LM agaroses have higher clarity (gel transparency)
than gels of standard agaroses. The gelling temperature of
LM agaroses is 24 to 28°C.
The structure of the polysaccharide is that of a galactan, formed by linking agarobioses by links 1-3, 1-4.
This chemical structure gives agaroses the capacity to form strong gels even at low temperatures. The
gels have a macroreticular structure with a very open mesh which can be adjusted simply by varying
the concentration of the agarose. The macroreticule structure of the agarose gel is formed by hydrogen
bonds, which makes the gel reversible, transforming the gel into a solution by heating. The absence
of ionic groups makes the gel a neutral structure. With no interaction, macromolecules can migrate
through the gel mesh, making the gel an efficient sieve for biological macromolecules.
Order Information
Optimize - LM Agarose
Cat. No. Name Description Price
HR8-092 LM Agarose 10 g $58.00
optimize crystallization grade reagents
57